TERMIUM Plus®

The Government of Canada’s terminology and linguistic data bank.

LIQUID COLUMN CHROMATOGRAPHY [20 records]

Filter results by subject field Alphabetical list of terms

Filter results by subject field

Alphabetical list of terms

Record 1 2022-07-20

English

Subject field(s)
  • Scientific Research Methods
  • Analytical Chemistry
  • Optics
CONT

Photometers and spectrophotometers that work in the visible range also are used as detectors in LC [liquid chromatography]. These devices are potentially useful both as general and selective detectors by utilizing well-known color reactions that can be performed with the column effluent and monitored by absorption photometry.

French

Domaine(s)
  • Méthodes de recherche scientifique
  • Chimie analytique
  • Optique
DEF

[...] mesure touchant à l'absorption d'un émission monochromatique[,] c'est-à-dire d'une longueur d'onde donnée[,] par une molécule en solution.

Spanish

Campo(s) temático(s)
  • Métodos de investigación científica
  • Química analítica
  • Óptica
CONT

La fotometría de llama se divide en dos categorías: fotometría de llama de emisión y de absorción. [...] La diferencia entre ambas es que mientras la fotometría de emisión se centra en la radiación emitida por la muestra, la fotometría de absorción mide la radiación absorbida por el analito.

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Record 2 2012-01-27

English

Subject field(s)
  • Analytical Chemistry
CONT

Liquid gel chromatography(LGC) is an established laboratory technique for the differential fractionation and separation of molecules. The underlying premise for simple LGC is that molecules elute from a gel bed in order of decreasing molecular weight. Generally, gel particles fill a chromatography column to form a bed. When the molecules to be partitioned, such as cells or proteins are introduced and driven through the bed by supplemental eluent, the molecules separate on the basis of molecular weight.

French

Domaine(s)
  • Chimie analytique

Spanish

Campo(s) temático(s)
  • Química analítica
DEF

Cromatografía de líquidos en la que la fase estacionaria es un gel.

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Record 3 2011-09-21

English

Subject field(s)
  • Scientific Instruments
  • Analytical Chemistry
  • Biotechnology
DEF

The tube and the stationary phase contained within, through which the mobile phase passes.

CONT

A chromatographic column, usually made of glass, is held vertically and is uniformly packed with a solvent. The sample is usually applied to the top, and the chromatogram is developed by passing one or several solvents through the porous bed from top to bottom.

OBS

A cylindrical column, used in chromatography, to hold a solid adsorbent, support, or a liquid stationary phase, and through which the mobile phase passes.

OBS

chromatographic column: term extracted from the "Disarmament and Peace Keeping" glossary with the permission of the United Nations Office at Geneva.

PHR

Column flowrate.

PHR

Dummy, high performance, large-bore, narrow-bore, symmetrical column.

French

Domaine(s)
  • Instruments scientifiques
  • Chimie analytique
  • Biotechnologie
DEF

Tube de diamètre et longueur variables, en verre, métal, ou autre substance, à l'intérieur duquel s'opèrent les séparations chromatographiques.

CONT

Une colonne de chromatographie en phase gazeuse classique est un tube en acier inoxydable, en verre ou en nickel, d'un diamètre intérieur de l,5 à 4mm et d'une longueur de 0,5 à 6 m. Ses dimensions obligent en général à lui donner une forme coudée, souvent hélicoïdale.

OBS

colonne chromatographique : terme tiré du lexique «Désarmement et Maintien de la Paix» avec l'autorisation de l'Office des Nations Unies à Genève.

PHR

La colonne se charge.

PHR

Soumettre la colonne à une percolation.

PHR

Commutation, effet extra, efficacité, extrémité, longévité, mise en équilibre, mise en série, optimisation, rayon d'enroulement, remplissage, tassement, température affichée, température contrôlée, tête, volume mort (de, d'une, des, de la) colonne.

PHR

Colonne analytique, classique, métallique, montée verticalement, moyennement chargée, perméable aux gaz.

PHR

Post-, pré-colonne.

Key term(s)
  • colonne séparatrice

Spanish

Campo(s) temático(s)
  • Instrumentos científicos
  • Química analítica
  • Biotecnología
DEF

Aparato a modo de [tubo que se utiliza] para la separación de uno o más componentes de una mezcla física mediante un relleno de material adsorbente; sus distintos componentes quedan retenidos preferentemente según diferentes velocidades de adsorción, produciéndose una separación.

CONT

La fase móvil de una columna cromatográfica está constituida por un líquido.

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Record 4 2011-07-13

English

Subject field(s)
  • Analytical Chemistry
OBS

Due to the drop in pressure that occurs in high-pressure liquid chromatography(HPLC) as the solvent passes from the column to the detector, any dissolved gases(most commonly air) are partially released as bubbles within the detector, producing anomalous and misleading results. To avoid this problem, solvents are degassed before use, either by a nitrogen purge or by heating and stirring under vacuum.

French

Domaine(s)
  • Chimie analytique

Spanish

Campo(s) temático(s)
  • Química analítica
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Record 5 2011-05-31

English

Subject field(s)
  • Analytical Chemistry
DEF

A procedure for chromatographic separation in which the mobile phase is passed through the chromatographic bed after the application on the sample.

OBS

The mixture is applied at the head of the column or as a spot on paper and thin layer. On the chromatogram thus developed, the solutes give either separate spots as in paper chromatography and thin-layer chromatography or a series of peaks on a chart as in gas chromatography and high-performance liquid chromatography.

French

Domaine(s)
  • Chimie analytique
DEF

Méthode de séparation chromatographique dans laquelle un éluant percole à travers le lit chromatographique après introduction de l'échantillon.

CONT

Que ce soit sur papier ou sur couche mince, on peut opérer les séparations suivant deux dimensions (axes de migration rectangulaires), mais la chromatographie sur couche mince est susceptible de variantes basées sur l'effet d'un gradient de composition du substrat (chromatographie à gradient d'élution), et même sur l'introduction de pressions assez importantes pour faire cheminer le véhicule liquide.

Spanish

Campo(s) temático(s)
  • Química analítica
DEF

Cromatografía en la que se hace pasar un eluyente a través de la fase estacionaria, después de haber introducido en ella la mezcla que se va a separar.

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Record 6 2011-05-24

English

Subject field(s)
  • Analytical Chemistry
CONT

All chromatographic systems involving passage of a mobile liquid phase over a solid adsorbent are forms of liquid-solid chromatography. These are all classified as adsorption chromatography, and include conventional column methods, dry-column chromatography, and thin layer chromatography.

Key term(s)
  • solid-liquid chromatography

French

Domaine(s)
  • Chimie analytique
DEF

Chromatographie dans laquelle la phase mobile est un liquide et la phase stationnaire est un solide.

CONT

La chromatographie liquide-solide [...] présente l'avantage de disposer de phases stationnaires très stables, très élaborées et offertes à des prix abordables.

Spanish

Campo(s) temático(s)
  • Química analítica
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Record 7 2008-10-10

English

Subject field(s)
  • Biochemistry
  • Dietetics
  • Food Additives
CONT

The initial solubilization of yolk platelets demonstrated that the lipid was present in the soluble fraction containing lipovitellin and phosvitin. These two proteins have been resolved and the lipovitellin fraction was shown to exhibit the absorption maximum at 375 nm that characterizes DCD [dorsalizing cytoplasmic determinant]. The lipovitellin was purified and its total lipids extracted and separated on HPLC [high-performance liquid chromatography]. A lipid was found that has identical elution properties from a C18 reverse HPLC column and mass as DCD. Therefore, lipovitellin, a zinc protein known to contain 17% lipid by weight, is the oocyte storage protein for DCD.

French

Domaine(s)
  • Biochimie
  • Diététique
  • Additifs alimentaires

Spanish

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Record 8 2008-10-10

English

Subject field(s)
  • Chemical Elements and Compounds
CONT

The use of HPLC [high performance liquid chromatography] to determine the saturate content of heavy petroleum products.... The refractive index response factors for several pure saturate fractions isolated from heavy petroleum products were determined using a µ-Bondapak aminopropylmethylsilyl bonded amorphous silica HPLC column.

CONT

Column - Water carbohydrate analysis [trademark] partition column. Aminopropylmethylsilyl bonded amorphous silica, 300 mm x 3.9 mm (ID) or equivalent.

French

Domaine(s)
  • Éléments et composés chimiques

Spanish

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Record 9 2004-03-04

English

Subject field(s)
  • Analytical Chemistry
DEF

A chromatographic technology used to separate and quantitate mixtures of substances in solution.

OBS

The sample is injected into a moving stream of solvent, which flows through a column and detector. The passage through the column separates the sample compounds by one of four different methods: adsorption, partition, ion exchange, or size exclusion. The solvent is pumped through the system at a controlled pressure, which can be varied between 500 and 5000 psi. With the particular column and elution method used for the assay, the elution times relative to the internal standard characterize the compounds and are used to identify them. The peak areas relative to the internal standard are proportional to their concentration in the sample and are used to quantitate them.

CONT

A special form of liquid chromatography, high-performance liquid chromatography(HPLC), has become especially important for the separation of complex mixtures of nonvolatile materials. Essentially, it involves the use of very small particle size in column packing, with high pressure to increase the rate of flow and shorten the separation time to a few minutes.

OBS

One of the most significant advances in chromatography in recent years has been the introduction of high performance liquid chromatography. Originally intended for the separation of small organic molecules that were soluble in non-aqueous solvents, the technique rapidly developed into a form suitable for the separation of larger, biological compounds soluble in aqueous solvents. In recent years, following the development of macroporous supports, the technique has become applicable to molecules as large as proteins and nucleic acids.

OBS

The term high pressure liquid chromatography (now obsolete) was used in the early stages of development of the technique since the quality of the columns was such that high pressures were needed to force the liquid through.

Key term(s)
  • high speed liquid chromatography
  • high-pressure high-speed liquid chromatography

French

Domaine(s)
  • Chimie analytique
DEF

Technique qui permet d'obtenir une séparation de mélanges complexes selon le même principe que la chromatographie liquide classique (échange d'ions, tamisage moléculaire, ou interaction hydrophobe) mais en des temps très courts grâce à l'emploi de hautes pressions.

OBS

Les avantages de l'HPLC [«high-performance liquid chromatography»] que sont la rapidité et la possibilité d'automatiser totalement l'analyse et le traitement des données (mise en mémoire et traitement informatique des courbes chromatographiques), sont susceptibles de conduire cette technique à remplacer, au moins dans certaines applications, l'électrophorèse classique actuelle.

CONT

La chromatographie liquide à haute performance (HPLC) est une méthode récente. L'évolution technologique a permis de réaliser des résines sphériques de taille allant jusqu'à 5 microns, ce qui leur donne des propriétés mécaniques telles qu'elles supportent de forts débits ainsi que la haute contre-pression qui en résulte. On réalise ainsi des séparations beaucoup plus rapides avec une meilleure résolution. L'appareil est constitué d'une colonne en acier inoxydable d'une longueur de l'ordre de 200 mm et de diamètre intérieur de 4 mm, remplie de particules de taille inférieure à 20 mm; cette colonne est précédée d'une pompe à haute pression, permettant d'injecter le solvant sous une pression de plusieurs dizaines de bars. Des microvannes assurent l'introduction de quelques microlitres de solution à analyser, en tête de colonne.

PHR

Chromatographie liquide à haute performance multidimensionnelle.

Key term(s)
  • chromatographie rapide en phase liquide

Spanish

Campo(s) temático(s)
  • Química analítica
CONT

CLAR es una técnica analítica en la que una fase móvil líquida transporta una muestra a través de una columna que contiene una fase líquida estacionaria. La interacción de la muestra con la fase estacionaria retiene de modo selectivo los compuestos individuales y permite la separación de los componentes de la muestra [...]

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Record 10 2003-11-07

English

Subject field(s)
  • Analytical Chemistry
DEF

A form of gas chromatography in which the fixed phase(column packing) is a liquid solvent distributed on an inert solid support.

OBS

The physical states of the mobile and stationary phases give rise to four basic types of chromatography: gas-liquid chromatography (GLC), gas-solid chromatography (GSC), liquid-liquid chromatography (LLC), and liquid-solid chromatography (LSC). Of the four basic types of chromatography, the two gas systems are independent and not subject to subdivisions. Thus the terms "gas-liquid chromatography" and "gas-solid chromatography" adequately identify the techniques involved. The liquid systems are not as easily described and are not mutually exclusive, which can lead to considerable confusion.

PHR

Open tubular gas-liquid chromatography.

French

Domaine(s)
  • Chimie analytique
DEF

Chromatographie en phase gazeuse dans laquelle la phase fixe est constituée par un liquide imprégnant un support solide inerte.

OBS

Dans cette définition et dans celle de la chromatographie gaz-solide, le premier mot spécifie la nature de la phase mobile et le second la phase stationnaire. Les phases stationnaires liquides sont déposées sur des solides, mais le support solide affecte le processus chromatographique. Dans un classement selon la nature des phases, le terme relatif à l'effet prédominant doit être choisi.

OBS

chromatographie gaz-liquide : terme normalisé par l'AFNOR.

Spanish

Campo(s) temático(s)
  • Química analítica
DEF

Cromatografía de gases en la que la fase estacionaria es un líquido soportado en un sólido.

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Record 11 2002-03-21

English

Subject field(s)
  • Analytical Chemistry
  • Biotechnology
DEF

A liquid which is relatively nonvolatile at the column temperature and is sorbed on the solid support(employed in gas-liquid and liquid-liquid chromatography) where it acts as a solvent for the sample. The separation achieved differs with differences in solutility of the various components of the sample in the liquid phase.

OBS

Thin-film liquid stationary phases can be prepared for separation by liquid-liquid partition chromatography. The film, commonly water, is supported on materials such as silica gel or diatomaceous earth, as in column chromatography. Either silica gel or diatomaceous earth may be silanized to convert the surfaces to nonpolar methyl groups for reversed-phase thin-layer chromatography.

PHR

Nonpolar, polar liquid phase.

French

Domaine(s)
  • Chimie analytique
  • Biotechnologie
DEF

En chromatographie gazeuse, liquide dont la tension de vapeur est relativement basse à la température de la colonne, qui est adsorbé sur le support solide et qui joue le rôle de solvant vis-à-vis de l'échantillon. La séparation repose sur la différence de solubilité des différents constituants de l'échantillon dans la phase liquide.

Spanish

Campo(s) temático(s)
  • Química analítica
  • Biotecnología
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Record 12 2000-10-19

English

Subject field(s)
  • Biochemistry
DEF

Type of high performance liquid chromatography that uses a gel filtration column.

French

Domaine(s)
  • Biochimie

Spanish

Save record 12

Record 13 1999-09-15

English

Subject field(s)
  • Biochemistry
DEF

In chromatography, volume of liquid required to remove a given component of a mixture from the column.

French

Domaine(s)
  • Biochimie
CONT

Dans le cas de particules non sphériques, le volume d'élution est directement proportionnel au rayon de Stokes, c'est-à dire au rayon d'une particule sphérique de propriétés hydrodynamiques équivalentes.

Spanish

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Record 14 1999-09-15

English

Subject field(s)
  • Biochemistry
DEF

Diagrammatic representation of the amounts of material eluted from a liquid chromatography column, and the sequence and position in which they occur.

French

Domaine(s)
  • Biochimie
CONT

Spectra Physics est en mesure de fournir des logiciels adaptés aux besoins des utilisateurs de chromatographe liquide ou gaz. Ces programmes en Basic peuvent être utilisés sur les intégrateurs SP 4270/4200 : calcul du nombre de plateaux de votre colonne, tracé de la courbe d'élution.

Spanish

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Record 15 1999-04-23

English

Subject field(s)
  • Analytical Chemistry
DEF

A device by means of which a liquid or gaseous sample is introduced directly into the chromatographic column or is swept into the column by the mobile phase.

CONT

An injection port is necessary in all g. c. [gas chromatography] and h. p. l. c. [high-performance liquid chromatography] systems in which syringe injection is employed. The injection port joins the top of the column to the mobile phase supply line, and has a third outlet through a small orifice covered by a self-sealing septum. Injection of samples is done by inserting the hypodermic syringe containing the sample through this septum so that the point of the needle is immediately above the head of the column. Design of the injection port must be such that it can be maintained at a uniform temperature and will possess a very small dead volume.

Key term(s)
  • sample injector
  • sample port

French

Domaine(s)
  • Chimie analytique
CONT

Chambre d'injection pour liquides ou solutions. C'est le système le plus utilisé [...] le gaz porteur, de préférence préchauffé, entre dans une chambre chauffée, obturée par une pastille d'élastomère, le septum, qui assure l'étanchéité. À l'aide d'une seringue hypodermique de petite capacité, on pique au travers de la membrane, de telle manière que l'extrémité de l'aiguille arrive au-dessous du niveau de l'arrivée du gaz porteur, puis on pousse le piston pour réaliser l'injection. La condition demandée à la chambre d'injection est d'avoir un volume interne aussi petit que possible, pour limiter les volumes morts de l'appareil. Pour assurer la vaporisation instantanée de l'échantillon, l'injecteur est habituellement maintenu à une température au moins 30 °C plus élevée que celle du four contenant la colonne.

OBS

L'injecteur à liquides classiques comprend: un écrou à ailettes pour serrage et refroidissement du septum, le septum, le bloc chauffé, la chambre de vaporisation, le capillaire de préchauffage, le raccord de colonne.

Spanish

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Record 16 1999-03-08

English

Subject field(s)
  • Analytical Chemistry
CONT

Any regular change occurring in the baseline signal from the detector in g. c. [gas chromatography] or h. p. l. c. [high-performance liquid chromatography]. It commonly arises from changes in the flow rate of the mobile phase or from deterioration of the detector such as might occur if the stationary phase is bleeding off the column.

OBS

[Detector drift] is represented as a continuous up-scale or down-scale excursion of the baseline, which tends to camouflage both noise and small peaks.

Key term(s)
  • base-line drift

French

Domaine(s)
  • Chimie analytique
Key term(s)
  • déplacement de la ligne de base

Spanish

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Record 17 1998-05-14

English

Subject field(s)
  • Analytical Chemistry
DEF

Chromatography in which the inner open-tube column wall itself, a liquid or an active solid, respectively, supported on the column wall, acts as the stationary phase.

Key term(s)
  • capillary chromatography
  • chromatography with open tubular columns

French

Domaine(s)
  • Chimie analytique
Key term(s)
  • chromatographie à colonne capillaire
  • chromatographie capillaire
  • chromatographie capillaire à tube ouvert

Spanish

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Record 18 1996-05-13

English

Subject field(s)
  • Analytical Chemistry
DEF

A device for the extremely sensitive measurement of the refractive index of liquids; used as a detector in liquid chromatography.

CONT

The most universal continuous detector for liquid chromatography is the differential refractometer. This detector is universal only to the extent that one can select an eluent the refractive index of which differs from that of the solutes. Pure eluent flows through one cell of the detector while column effluent flows through the other cell. When a solute with a refractive index different from that of the solvent enters the detector, an imbalance occurs, which is transmitted to the recorder. The differential refractometers are relatively insensitive to trace and minor components, and detector stability is greatly affected by small temperature and flow fluctuations, thus requiring very good flow and temperature controls.

French

Domaine(s)
  • Chimie analytique
CONT

Le réfractomètre différentiel mesure en continu la différence d'indice de réfraction entre la phase mobile et l'effluent de la colonne chromatographique. La limite de détection dépend à la fois de la nature du soluté et de celle de la phase éluante.

Spanish

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Record 19 1996-03-25

English

Subject field(s)
  • Analytical Chemistry
DEF

In liquid chromatography, peak elution volume [is] defined as the volume of eluent entering the column from the start of elution to the appearance of the peak maximum.

French

Domaine(s)
  • Chimie analytique
DEF

Le volume d'éluant entrant dans la colonne entre le début de l'élution et la sortie du maximum du pic. Cette expression s'applique seulement à la chromatographie liquide. Elle n'inclut ni le volume d'effluent recueilli lorsque l'échantillon est introduit dans la colonne, ni le volume du détecteur éventuellement utilisé.

Spanish

Save record 19

Record 20 1996-03-12

English

Subject field(s)
  • Analytical Chemistry
DEF

In high performance liquid chromatography, the packing procedure performed by pumping a suspension of the sorbent prepared in a liquid medium of the same density into a column at a pressure much higher than operating pressure.

French

Domaine(s)
  • Chimie analytique

Spanish

Save record 20

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