TERMIUM Plus®

The Government of Canada’s terminology and linguistic data bank.

LUCIFERASE [7 records]

Record 1 2017-12-15

English

Subject field(s)
  • Scientific Research Methods
CONT

[The] method, called bioluminescence resonance energy transfer(BRET), uses a bioluminescent luciferase that is genetically fused to one candidate protein, and a green fluorescent protein mutant fused to another protein of interest. Interactions between the two fusion proteins can bring the luciferase and green fluorescent protein close enough for resonance energy transfer to occur, thus changing the color of the bioluminescent emission.

French

Domaine(s)
  • Méthodes de recherche scientifique
CONT

[...] les techniques de transfert d’énergie de résonance de bioluminescence (BRET), pour étudier la dynamique de signalisation en temps réel dans des cellules vivantes.

Spanish

Save record 1

Record 2 2015-03-02

English

Subject field(s)
  • Biochemistry
  • Biophysics
CONT

Bioluminescence in Renilla is due to the luciferase-catalyzed oxidation of luciferin. Anderson and Cormier have isolated a membrane-bounded, subcellular particle containing luciferase, a luciferin-binding protein with its associated luciferin and a green fluorescent protein all of which are necessary for bioluminescence and its control. When subjected to hypotonic solutions of calcium, these particles produce a flash of green light identical to the in vivo luminescence of Renilla. These particles, identified as the apparent sites of bioluminescence in Renilla, have been termed lumisomes.

French

Domaine(s)
  • Biochimie
  • Biophysique
DEF

Organite générateur de bioluminescence.

CONT

Ce sont des organes pratiquement plans [dans les Annélides Polynoinae] qui peuvent émettre de la lumière sous forme d'éclairs bien caractérisés [...], et c'est là un des cas les plus nets ou la réaction lumineuse est non seulement intracellulaire, mais encore localisée à des organites particuliers: les photosomes [...]

Spanish

Campo(s) temático(s)
  • Bioquímica
  • Biofísica
Save record 2

Record 3 2013-08-13

English

Subject field(s)
  • Biotechnology
  • Genetics
CONT

Neurons from the embryonic brain of Xenopus were transfected in vivo with a vector expressing luciferase cDNA [complementary DNA] using a simple lipofection procedure. Luciferase activity was monitored quantitatively, and the protein was immunolocalized in whole-mount embryonic brain.

French

Domaine(s)
  • Biotechnologie
  • Génétique
CONT

La lipofection voit également son importance augmenter, principalement en cancérologie dans le sillage des essais de Nabel et al. dont les résultats cliniques d'injections intramusculaires immunisantes ont été encourageants.

Spanish

Campo(s) temático(s)
  • Biotecnología
  • Genética
DEF

[Técnica de] introducción, en células eucariotas, de ADN, ARN o de otros compuestos, previamente encapsulados en liposomas.

Save record 3

Record 4 2011-08-10

English

Subject field(s)
  • Genetics
  • Biotechnology
CONT

Du Pont and Duke University collaborators have developed the Biolistic gene gun, a particle delivery system which employs microcarriers coated with genetic material to insert DNA into animal cells. The microcarriers are accelerated on macrocarriers in a stream of helium to fire the microcarriers into external tissue or, using surgery, into the livers of mice. The system has been used to insert genes encoding the enzyme, firefly luciferase, into mice and the next stage is likely to be insertion of a therapeutically useful gene into animal liver cells which would show the procedure to be of potential use in gene therapy.

OBS

Sometimes blank bullets containing gold particles are used instead of pressured helium to carry the DNA into animal or plant cells.

French

Domaine(s)
  • Génétique
  • Biotechnologie
DEF

Dispositif d'injection de gènes dans les tissus.

CONT

L'ADN peut être également fixé sur des microparticules d'or, projetées dans les tissus par un 'pistolet à gènes' (gene gun) que commercialise la firme américaine Agracetus.

Spanish

Campo(s) temático(s)
  • Genética
  • Biotecnología
DEF

Dispositivo que se utiliza para introducir ADN en células a través del bombardeo de tejidos con micropartículas de metal recubiertas con ADN.

CONT

Un método de transformación utilizado en algunos cultivos, es el “cañón de genes” o “pistola de genes”, instrumento que permite introducir genes en plantas mediante aceleración (o "bombardeo") de micro proyectiles de oro o tungsteno, recubiertos con el fragmento de ADN a incorporar.

Save record 4

Record 5 2010-02-03

English

Subject field(s)
  • Photoelectricity and Electron Optics
  • Biological Sciences
CONT

A biophoton ... is a photon of light emitted from a biological system and detected by biological probes as part of the general weak electromagnetic radiation of living biological cells.

OBS

Biophotons and their study should not be confused with bioluminescence, a term generally reserved for higher intensity luciferin/luciferase systems.

Key term(s)
  • bio-photon
  • bio photon

French

Domaine(s)
  • Photo-électricité et optique électronique
  • Sciences biologiques
CONT

Le biophoton [...] est un photon d'origine biologique n'étant pas issu de produits d'une réaction enzymatique spécifique. Il s'agit donc d'une chimiluminescence d'origine biologique qui se distingue de la bioluminescence par son abscence de mécanisme enzymatique dédié et par une magnitude ou intensité ultra-faible (de l'anglais, ultra-weak spontaneous photon emission, ou parfois plus simplement ultra-weak photon emission).

OBS

Fritz-Albert Popp, inventeur du terme, définit les biophotons par l'intensité de leur émission à la surface des tissus vivants, qui est de l'ordre de 10 à 1000 photons par centimètre carré et par seconde.

Key term(s)
  • bio-photon
  • bio photon

Spanish

Save record 5

Record 6 2006-08-28

English

Subject field(s)
  • Biochemistry
CONT

Analysis of shut-off mechanism of luciferase activity during the growth phase transition in Escherichia coli

French

Domaine(s)
  • Biochimie
CONT

L'antibiotique gentamicine a en effet la faculté de forcer la machinerie de synthèse des protéines à passer outre les signaux d'arrêt de synthèse et permet ainsi de restaurer l'expression de la protéine.

Spanish

Save record 6

Record 7 2003-07-14

English

Subject field(s)
  • Chemical Elements and Compounds
  • Chemistry
CONT

Short-chain aldehydes are produced from fatty hydroperoxides but are also found in vegetals. Long-chain aldehydes occur in free form but are frequently included in complex lipids in the form of vinyl ether (plasmalogen analogs of glycerides or phospholipids).

CONT

The bioluminescent reaction of luciferase isolated from "Vibrio harveyi, "strain M17, was initiated by mixing the intermediate luciferase-bound flavin-4alpha-hydroperoxide, purified in advance, with a long chain aldehyde(dodecanal or octanal) at-4°C.

French

Domaine(s)
  • Éléments et composés chimiques
  • Chimie
CONT

Mise au point d'une technique de dosage des aldéhydes à longue chaîne dans les milieux biologiques par chromatographie en phase gazeuse couplée à la spectrométrie de masse.

Spanish

Save record 7

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