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MICROTITER PLATE [3 records]

Record 1 2010-04-09

English

Subject field(s)
  • Biotechnology
DEF

Individual primary recombinant clones(hosted in phage, cosmid, YAC, or other vector) that are placed in two-dimensional arrays in microtiter dishes. Each primary clone can be identified by the identity of the plate and the clone location(row and column) on that plate. Arrayed libraries of clones can be used for many applications, including screening for a specific gene or genomic region of interest as well as for physical.

OBS

Glossary in molecular biology (Portions of the glossary text were taken directly or modified from definitions in the U.S. Congress Office of Technology Assessment document.

French

Domaine(s)
  • Biotechnologie
OBS

Les clones sont disposés en rangs et en colonnes (grilles) dans une boîte de Pétri. Ce type de banque est différent des banques génomiques où les clones sont dispersés sans ordre dans la boîte de Pétri.

OBS

Proposition faite par un spécialiste en biologie moléculaire à la GRC.

Spanish

Save record 1

Record 2 2006-03-16

English

Subject field(s)
  • Immunology
CONT

We have generally found that irradiated merrotitre plates have a greater capacity than non-irradiated plates to bind allergen or whole cells. Once bound, the allergen can become detached from the plate and this is particularly critical if it occurs during the assay... One solution to this problem is to link the antigen to the plate via an antibody in a sandwich ELISA(SELISA). Polyclonal rabbit and mouse monoclonal antibodies have been used to bind allergens to microtiter plates.

French

Domaine(s)
  • Immunologie
CONT

Techniques ELISA [...] On parle de techniques immunoenzymatiques directes, indirectes, en sandwich, avec ou sans compétition [...] la méthode en sandwich (comporte les) étapes suivantes. Les anticorps spécifiques sont fixés passivement sur le support solide. Puis on dépose l'échantillon testé; l'antigène présent se fixera sur l'anticorps. On applique alors l'anticorps spécifique, éventuellement lié à une enzyme, suivi de l'anticorps anti-immunoglobuline spécifique de l'espèce où est produit l'anticorps [...] On ajoute enfin le substrat de l'enzyme.

OBS

Pluriel : des sandwichs; des sandwiches.

OBS

sandwichs (pl.) : Cette graphie, puisée des Rectifications de l'orthographe recommandées par le Conseil supérieur de la langue française, est attestée dans le Petit Robert (2006).

Spanish

Save record 2

Record 3 1999-02-03

English

Subject field(s)
  • Biotechnology
CONT

In the case of the high-speed colony picker, the machinery consists of two tables capable of computer-controlled movement positioned underneath a pair of plungers with a rotating carousel wheel in between that is fitted with 24 reusable needles. To operate the system, a Petri dish containing colonies of yeast or bacteria is placed on one table, and a 96-well microtiter plate is placed on the other. Guided by a digital image that shows the location of colonies in the dish, the first table moves a colony directly underneath a plunger which at the same time is being armed with a needle from the carousel. On a signal from a computer, the plunger dips the needle into the colony and immediately retracts it with "picked" cells. The carousel then rotates the needle over to the second plunger and the table bearing the microtiter plate moves until the correct well is directly underneath it. The computer activates the second plunger, dipping the needle into the growth medium where some of the cells will be deposited. After retraction from the growth medium, the needle is rotated by the carousel through a tank filled with sterilizing liquid and made ready for reuse.

French

Domaine(s)
  • Biotechnologie
CONT

Seule la sociéte Hybrid a développé un appareil pour le repiquage automatique des colonies, le Colony Picker. Doté d'un système optique sophistiqué couplé à un bras multiaxe, il repique les plages de lyse ou les colonies bactériennes des boîtes de Pétri et les distribue dans un milieu pour la culture cellulaire. Sa très forte capacité permet d'augmenter très sensiblement la cadence dans les laboratoires de séquençage.

OBS

Proposition par analogie avec repiqueuse de plants en agriculture.

Spanish

Campo(s) temático(s)
  • Biotecnología
Save record 3

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