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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

Pyrosequencing is a method of DNA sequencing that differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release and the generation of light on nucleotide incorporation, rather than chain termination with dideoxynucleotides.  2, record 1, English, - pyrosequencing

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

Le pyroséquençage est une technique de séquençage de l'ADN qui permet d’effectuer un séquençage rapide et à moindre coût qu'un séquençage par la méthode de Sanger. En effet, cette technique ne nécessite pas de clonage (donc gain de temps et d'argent), et permet une lecture directe de la séquence obtenue après le séquençage.  2, record 1, French, - pyros%C3%A9quen%C3%A7age

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

Enzyme of the hydrolase class that catalyze the hydrolytic cleavage of polynucleotides.  2, record 2, English, - endonuclease

Record number: 2, Textual support number: 1 OBS

Examples are deoxyribonuclease, endodeoxyribonuclease, and endoribonuclease. Some members of this group are important in the metabolism and repair of DNA and RNA.  2, record 2, English, - endonuclease

Record number: 2, Textual support number: 2 OBS

A specific class of deoxyribonucleases known as restriction endonucleases cleaves DNA at specific nucleotide sequences. These enzymes protect the cells from foreign DNA, but they do not act on the host DNA. They have been used extensively in DNA sequencing and recombinant DNA technology.  2, record 2, English, - endonuclease

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 DEF

Enzyme qui clive les liaisons internes d'un acide nucléique. [Définition normalisée par l'AFNOR et reproduite avec son autorisation.]  2, record 2, French, - endonucl%C3%A9ase

Record number: 2, Textual support number: 1 OBS

Elle peut être spécifique d'un ARN (endoribonucléase) ou d'un ADN (endodésoxyribonucléase) simple ou double brin. [Observation reproduite avec l'autorisation de l'AFNOR.]  2, record 2, French, - endonucl%C3%A9ase

Record number: 2, Textual support number: 2 OBS

endonucléase : terme normalisé par l'AFNOR.  3, record 2, French, - endonucl%C3%A9ase

Record 2,  Textual support,  Spanish

Record number: 2, Textual support number: 1 DEF

Cada una de las enzimas de la clase hidrolasas, que catalizan la escición al azar de uniones fosfodiester de los ácidos nucleicos, en posiciones interiores de la molécula.  2, record 2, Spanish, - endonucleasa

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 CONT

We report a simple and reliable protocol for nucleotide sequencing using the Sanger dideoxy technique on linearized double-stranded DNA molecules with specific oligonucleotide primers. The method is demonstrated for restriction fragments cloned into the plasmid vectors pSP64 and pSP65 using two vector-specific primers, the M13 reverse primer and a new SP6 primer, flanking the multiple cloning site.  1, record 3, English, - reverse%20primer

Record number: 3, Textual support number: 1 OBS

Term and context extracted from the BIOSIS data base.  1, record 3, English, - reverse%20primer

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 OBS

reverse transcription = transcription inverse; reverse mutation = mutation inverse.  1, record 3, French, - amorce%20inverse

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 CONT

LKB Macrophor DNA/RNA Sequencing System : For sequencing of DNA and RNA. New separation techniques involve the generation of a set of oligonucleotides having one end in common and differing in length in steps of one nucleotide, followed by electrophoretic separation of the oligonucleotides in a denaturing polyacrylamide gel.  1, record 4, English, - DNA%2FRNA%20sequencing%20system

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Système séquenceur d'ADN/ARN Macrophor LKB : Des nouvelles techniques de séparation impliquent la génération d'un ensemble d'oligonucléotides ayant une extrémité en commun et différant en longueur en phases d'un nucléotide, suivi d'une séparation électrophorétique d'oligonucléotides dans un gel de polyacrylamide dénaturé.  1, record 4, French, - syst%C3%A8me%20s%C3%A9quenceur%20d%27ADN%2FARN

Record 4,  Textual support,  Spanish

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 DEF

[DNA sequencing treatment in which a] subsample(...) is incubated with DNA polymerase and with only three of the four nucleotide building blocks.  1, record 5, English, - minus%20system

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 DEF

[méthode pour établir la séquence de l'ADN selon laquelle les] échantillons (...) sont incubés en présence d'une ADN-polymérase et de seulement trois des quatre nucléotides.  2, record 5, French, - syst%C3%A8me%20moins

Record 5,  Textual support,  Spanish