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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

A more detailed map of a large YAC [yeast artificial chromosome] insert can be produced by subcloning, a process in which fragments of the original insert are cloned into smaller-insert vectors. Because some YAC regions are unstable, large-capacity bacterial vectors(i. e. those that accommodate large inserts) are also being developed.  1, record 1, English, - large%2Dcapacity%20bacterial%20vector

Record 1,  Textual support,  French

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

We therefore propose to undertake the identification, assembly and test of the structural elements necessary for the proper function and stability of a mammalian artificial chromosome(MAC). To achieve this goal, we will create a chimaera between an existing Yeast Artificial Chromosome(YAC) and selected human and other mammalian genomic sequences. The stability and autonomous replication of the MAC in human cells will be evaluated by a variety of assays, both before and after the addition of candidate origins, telomeres and centromeres.  2, record 2, English, - mammalian%20artificial%20chromosome

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

C'est ainsi qu'est né le concept de minichromosomes ou MAC (mammalian artificial chromosomes). Ceux-ci pourraient maintenir de grands morceaux d'ADN parallèlement aux chromosomes de la cellule sans risque d'intégration ou de recombinaison avec des virus et sans induire de réponse immunitaire.  3, record 2, French, - chromosome%20mammif%C3%A8re%20artificiel

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 DEF

Individual primary recombinant clones(hosted in phage, cosmid, YAC, or other vector) that are placed in two-dimensional arrays in microtiter dishes. Each primary clone can be identified by the identity of the plate and the clone location(row and column) on that plate. Arrayed libraries of clones can be used for many applications, including screening for a specific gene or genomic region of interest as well as for physical.  1, record 3, English, - arrayed%20library

Record number: 3, Textual support number: 1 OBS

Glossary in molecular biology (Portions of the glossary text were taken directly or modified from definitions in the U.S. Congress Office of Technology Assessment document.  2, record 3, English, - arrayed%20library

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 OBS

Les clones sont disposés en rangs et en colonnes (grilles) dans une boîte de Pétri. Ce type de banque est différent des banques génomiques où les clones sont dispersés sans ordre dans la boîte de Pétri.  1, record 3, French, - banque%20de%20clones%20dispos%C3%A9s%20en%20grille

Record number: 3, Textual support number: 2 OBS

Proposition faite par un spécialiste en biologie moléculaire à la GRC.  1, record 3, French, - banque%20de%20clones%20dispos%C3%A9s%20en%20grille

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 CONT

A map of human chromosome 3 which integrates both physical and genetic data has been developed from the fusion of two large collections of markers and corresponding yeast artificial chromosome(YAC) clones.  3, record 4, English, - yeast%20artificial%20chromosome

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Clonage de grands fragments d'ADN sous forme de chromosomes artificiels de levure (YAC, Yeast Artificial Chromosomes). Le principe de ce système consiste à munir de grands fragments d'ADN exogène (humain par exemple) de tous les éléments qui permettent leur propagation dans la levure [...]. Ces constructions sont alors répliquées par la levure comme s'il s'agissait d'un chromosome endogène. On peut ainsi construire des banques dans lesquelles chaque clone de levure contient un segment d'ADN humain de grande taille propagé sous forme de chromosome artificiel.  4, record 4, French, - chromosome%20artificiel%20de%20levure

Record 4,  Textual support,  Spanish

Record number: 4, Textual support number: 1 DEF

Cromosoma artificial que se emplea como vector de clonación de grandes fragmentos de ADN de otras especies en el genoma de la levadura.  1, record 4, Spanish, - cromosoma%20artificial%20de%20levadura

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 CONT

Clusters of genes with one per 15 or 30 kilobases were described, but they also noted that only a single cDNA was captured from YAC clones spanning a 6 megabase region of the q21 band by selection methods. Why so few genes were present in such a large region, which corresponded to a darkly staining Giemsa band, appeared to be an intriguing question and one that remained unresolved.  1, record 5, English, - Giemsa%20band

Record number: 5, Textual support number: 1 OBS

CGAT GENExpress. volume 3 number 1, June 1995. THE SULFOLOBUS SOLFATARICUS GENOME PROJECT. Mark A. Ragan. In November 1977, Carl Woese and his colleagues..  2, record 5, English, - Giemsa%20band

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 CONT

Les chromosomes métaphasiques humains présentent divers types de bandes selon le traitement auquel ils sont soumis (colorants, température, enzymes protéolytiques ou DNases). Les bandes G(iemsa) positives représentent environ 50% des bandes chromosomiques, l'autre moitié étant constituée de bandes G(iemsa) négatives. Ces dernières ne sont que les bandes R(everse) obtenues par dénaturation thermique. Quand aux bandes T(élomériques), identifiées par B Dutrillaux, elle forment un sous-ensemble des bandes R, à savoir les plus résistantes à la dénaturation, et sont localisées essentiellement au niveau des télomères.  1, record 5, French, - bande%20Giemsa

Record 5,  Textual support,  Spanish