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YEAST ARTIFICIAL CHROMOSOME [4 records]

Record 1 2010-08-11

English

Subject field(s)
  • Biotechnology
CONT

The molar fractions of guanine plus cytosine(GC) in DNA were determined for 36 yeast artificial chromosomes(YACs) which almost completely cover human chromosome band Xq28, a terminal reverse band, corresponding to about 8 Mb of DNA. This allowed the construction of the most complete compositional map to date of a chromosomal band...

OBS

Proceedings of the National Academy of Sciences, Volume 93, Number 03; Pages: 1298-1302

French

Domaine(s)
  • Biotechnologie
CONT

Les chromosomes métaphasiques humains présentent divers types de bandes selon le traitement auquel ils sont soumis (colorants, température, enzymes protéolytiques ou DNases). Les bandes G(iemsa) positives représentent environ 50 % des bandes chromosomiques, l'autre moitié étant constituée de bandes G(iemsa) négatives. Ces dernières ne sont que les bandes R(everse) obtenues par dénaturation thermique.

OBS

Elles sont obtenues par dénaturation thermique en solution saline, spécifiques des régions riches en guanine et en cytosine ainsi qu'en gènes de synthèse précoces.

Spanish

Save record 1

Record 2 2010-06-22

English

Subject field(s)
  • Biotechnology
CONT

A more detailed map of a large YAC [yeast artificial chromosome] insert can be produced by subcloning, a process in which fragments of the original insert are cloned into smaller-insert vectors. Because some YAC regions are unstable, large-capacity bacterial vectors(i. e. those that accommodate large inserts) are also being developed.

French

Domaine(s)
  • Biotechnologie

Spanish

Save record 2

Record 3 2010-04-12

English

Subject field(s)
  • Genetics
CONT

We therefore propose to undertake the identification, assembly and test of the structural elements necessary for the proper function and stability of a mammalian artificial chromosome(MAC). To achieve this goal, we will create a chimaera between an existing Yeast Artificial Chromosome(YAC) and selected human and other mammalian genomic sequences. The stability and autonomous replication of the MAC in human cells will be evaluated by a variety of assays, both before and after the addition of candidate origins, telomeres and centromeres.

French

Domaine(s)
  • Génétique
CONT

C'est ainsi qu'est né le concept de minichromosomes ou MAC (mammalian artificial chromosomes). Ceux-ci pourraient maintenir de grands morceaux d'ADN parallèlement aux chromosomes de la cellule sans risque d'intégration ou de recombinaison avec des virus et sans induire de réponse immunitaire.

Spanish

Save record 3

Record 4 2007-08-08

English

Subject field(s)
  • Genetics
CONT

A map of human chromosome 3 which integrates both physical and genetic data has been developed from the fusion of two large collections of markers and corresponding yeast artificial chromosome(YAC) clones.

French

Domaine(s)
  • Génétique
CONT

Clonage de grands fragments d'ADN sous forme de chromosomes artificiels de levure (YAC, Yeast Artificial Chromosomes). Le principe de ce système consiste à munir de grands fragments d'ADN exogène (humain par exemple) de tous les éléments qui permettent leur propagation dans la levure [...]. Ces constructions sont alors répliquées par la levure comme s'il s'agissait d'un chromosome endogène. On peut ainsi construire des banques dans lesquelles chaque clone de levure contient un segment d'ADN humain de grande taille propagé sous forme de chromosome artificiel.

Spanish

Campo(s) temático(s)
  • Genética
DEF

Cromosoma artificial que se emplea como vector de clonación de grandes fragmentos de ADN de otras especies en el genoma de la levadura.

Save record 4

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