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YEAST ENZYME [9 records]
Record 1 - internal organization data 2013-11-15
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- isoacceptor tRNA
1, record 1, English, isoacceptor%20tRNA
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- isoacceptor transfer RNA 2, record 1, English, isoacceptor%20transfer%20RNA
correct
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
In yeast, inosine(I) is found at position 34 of 7 different isoacceptor tRNA species. Using as substrates in vitro T7-transcripts of 31 plasmids carrying each natural or synthetic tRNA gene harbouring an anticodon with A-34, we have characterised a yeast enzyme that catalyses the conversion of A-34 to I-34. The base conversion occurs by a hydrolytic deamination-type reaction. This newly characterised tRNA : A-34 deaminase was partially purified from yeast. 1, record 1, English, - isoacceptor%20tRNA
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- ARNt isoaccepteur
1, record 1, French, ARNt%20isoaccepteur
correct, masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Terme qui décrit les différents tRNA (RNAt) spécifiques d'un même aminoacide. 2, record 1, French, - ARNt%20isoaccepteur
Record number: 1, Textual support number: 1 CONT
Tenant compte de ces faits, nous avons recherché les éléments d'identité responsables de l'arginylation spécifique chez la levure, en étudiant in vitro, les propriétés d'arginylation de toute une série de transcrits dérivés de l'ARNt-Asp, considéré comme un isoaccepteur pour l'arginine. En parallèle des expériences similaires ont été réalisées avec des transcrits dérivés de l'ARNt-Arg. 1, record 1, French, - ARNt%20isoaccepteur
Record 1, Spanish
Record 1, Textual support, Spanish
Record 2 - internal organization data 2012-10-22
Record 2, English
Record 2, Subject field(s)
- Genetics
- Biotechnology
Record 2, Main entry term, English
- ultrasonication
1, record 2, English, ultrasonication
correct
Record 2, Abbreviations, English
Record 2, Synonyms, English
- sonication 2, record 2, English, sonication
correct
Record 2, Textual support, English
Record number: 2, Textual support number: 1 DEF
Breaking up cells [or DNA molecules] with ultrasound. 3, record 2, English, - ultrasonication
Record number: 2, Textual support number: 1 CONT
Grow the bacteria in Burks nitrogen-free medium supplemented with 0. 05% yeast extract for 48 hrs. in shake flasks containing 1LTR medium use 1%(W/V) glucose, sterilised separately as the carbon source. Harvest the bacteria, break by ultrasonication or in the French pressure cell, ultracentrifuge and keep the supernatant from the lysate as enzyme. Dialyse against cold water overnight and concentrate in the cold room or refrigerator using dialysis against polyethylene glycol. 1, record 2, English, - ultrasonication
Record 2, French
Record 2, Domaine(s)
- Génétique
- Biotechnologie
Record 2, Main entry term, French
- ultrasonication
1, record 2, French, ultrasonication
correct, feminine noun
Record 2, Abbreviations, French
Record 2, Synonyms, French
- sonication 1, record 2, French, sonication
correct, feminine noun
- sonication aux ultra-sons 2, record 2, French, sonication%20aux%20ultra%2Dsons
correct, feminine noun
- sonication aux ultrasons 3, record 2, French, sonication%20aux%20ultrasons
correct, feminine noun
Record 2, Textual support, French
Record number: 2, Textual support number: 1 DEF
Rupture des cellules ou des molécules d’ADN par des ondes sonores à haute fréquence (ultrasons). 1, record 2, French, - ultrasonication
Record number: 2, Textual support number: 1 CONT
La séparation des têtes des flagelles par sonication aux ultra-sons nous a permis de déterminer que la P21b se localise sur le flagelle des spermatozoïdes alors que la P25b est associée à la tête. Lors d'une quantification par transfert électrophorique de type "Western" sur des spermatozoïdes de différents taureaux fertiles et sous-fertiles, nous avons observé que la quantité de P21b est comparable d'un sujet à l'autre, alors que la quantité de P25b montre une variabilité interindividuelle. 2, record 2, French, - ultrasonication
Record number: 2, Textual support number: 2 CONT
L'ADN cible sera fragmenté de façon mécanique (sonication aux ultrasons), enzymatique (digestion à l'aide d'ADN-ase) ou chimique (hydrolyse métal-ligand) pour que les fragments aient la taille idéale afin d'assurer l'efficacité de l'interaction avec les sondes immobilisées. 3, record 2, French, - ultrasonication
Record 2, Spanish
Record 2, Campo(s) temático(s)
- Genética
- Biotecnología
Record 2, Main entry term, Spanish
- ultrasonicación
1, record 2, Spanish, ultrasonicaci%C3%B3n
correct, feminine noun
Record 2, Abbreviations, Spanish
Record 2, Synonyms, Spanish
- sonicación 1, record 2, Spanish, sonicaci%C3%B3n
correct, feminine noun
Record 2, Textual support, Spanish
Record number: 2, Textual support number: 1 DEF
Disgregación de células o de moléculas de ADN por ondas ultrasónicas. 1, record 2, Spanish, - ultrasonicaci%C3%B3n
Record 3 - internal organization data 2012-05-18
Record 3, English
Record 3, Subject field(s)
- Biochemistry
- Brewing and Malting
- Biotechnology
Record 3, Main entry term, English
- zymase
1, record 3, English, zymase
correct
Record 3, Abbreviations, English
Record 3, Synonyms, English
Record 3, Textual support, English
Record number: 3, Textual support number: 1 DEF
A cell-free yeast extract containing the enzyme necessary for fermentation. 2, record 3, English, - zymase
Record 3, French
Record 3, Domaine(s)
- Biochimie
- Brasserie et malterie
- Biotechnologie
Record 3, Main entry term, French
- zymase
1, record 3, French, zymase
correct, feminine noun
Record 3, Abbreviations, French
Record 3, Synonyms, French
Record 3, Textual support, French
Record number: 3, Textual support number: 1 DEF
Enzyme catalysant les réactions de la fermentation alcoolique du glucose dans l'extrait de levure. 2, record 3, French, - zymase
Record 3, Spanish
Record 3, Campo(s) temático(s)
- Bioquímica
- Fabricación de cerveza y malta
- Biotecnología
Record 3, Main entry term, Spanish
- zimasa
1, record 3, Spanish, zimasa
correct, feminine noun
Record 3, Abbreviations, Spanish
Record 3, Synonyms, Spanish
Record 3, Textual support, Spanish
Record 4 - internal organization data 2012-02-03
Record 4, English
Record 4, Subject field(s)
- Biological Sciences
Record 4, Main entry term, English
- urease
1, record 4, English, urease
correct
Record 4, Abbreviations, English
Record 4, Synonyms, English
Record 4, Textual support, English
Record number: 4, Textual support number: 1 DEF
Enzyme that catalyzes the conversion of urea to ammonia and carbon dioxide. 2, record 4, English, - urease
Record number: 4, Textual support number: 1 CONT
It was therefore a major landmark in the history of enzyme research when, in 1897, Eduard Buchner succeeded in extracting from yeast cells that enzymes catalyzing alcoholic fermentation. This achievement clearly demonstrated that these important enzymes, which catalyze a major energy-yielding metabolic pathway, can function independently of cell structure. However, it was not until many years later that an enzyme was first isolated in pure crystalline form. This was accomplished by J. B. Sumner in 1926 for the enzyme urease isolated from extracts of the jack bean. Sumner presented evidence that the crystals consist of protein, and he concluded, contrary to prevailing opinion, that enzymes are proteins. 3, record 4, English, - urease
Record 4, French
Record 4, Domaine(s)
- Sciences biologiques
Record 4, Main entry term, French
- uréase
1, record 4, French, ur%C3%A9ase
correct, feminine noun
Record 4, Abbreviations, French
Record 4, Synonyms, French
Record 4, Textual support, French
Record number: 4, Textual support number: 1 DEF
Enzyme catalysant l'hydrolyse de l'urée en carbonate d'ammonium. Elle est présente chez les végétaux ou chez les microorganismes responsables de la décomposition de l'urée dans les urines exposées à l'air. L'enzyme est utilisée pour le dosage spécifique de l'urée dans les milieux biologiques. 2, record 4, French, - ur%C3%A9ase
Record number: 4, Textual support number: 1 CONT
Un très grand nombre de dosages, en particulier en analyse médicale, requièrent l'utilisation d'enzymes. Ces enzymes solubles peuvent être remplacées avantageusement par des enzymes insolubilisées sur des supports papier ou Nylon. [...] Beaucoup plus original est le développement d'électrodes biologiques, dites «électrodes à enzymes». Il s'agit en fait d'électrodes mixtes. Ainsi, dans le cas d'une électrode à urée, l'uréase adsorbée sur un gel convertit l'urée en ions ammonium et HCO3-. Une électrode sensible aux ions NH4+ permet la mesure directe de la quantité d'urée disponible (qui détermine en fait la vitesse de production d'ions NH4+). 3, record 4, French, - ur%C3%A9ase
Record 4, Spanish
Record 4, Campo(s) temático(s)
- Ciencias biológicas
Record 4, Main entry term, Spanish
- ureasa
1, record 4, Spanish, ureasa
correct, feminine noun
Record 4, Abbreviations, Spanish
Record 4, Synonyms, Spanish
Record 4, Textual support, Spanish
Record 5 - internal organization data 2011-07-22
Record 5, English
Record 5, Subject field(s)
- Biological Sciences
Record 5, Main entry term, English
- induced-fit theory
1, record 5, English, induced%2Dfit%20theory
correct
Record 5, Abbreviations, English
Record 5, Synonyms, English
- induced-fit hypothesis 2, record 5, English, induced%2Dfit%20hypothesis
correct
- cooperativity model 3, record 5, English, cooperativity%20model
correct
Record 5, Textual support, English
Record number: 5, Textual support number: 1 DEF
Theory according to which the binding of a substrate to an enzyme may cause conformational changes that align the catalytic groups in their correct orientations. 2, record 5, English, - induced%2Dfit%20theory
Record number: 5, Textual support number: 1 CONT
There is good evidence that yeast hexokinase, which phosphorylates glucose with the terminal phosphate of ATP, involves the induced fit theory. X-ray diffraction studies show that the binding of both the sugar and the nucleotide causes extensive changes in tertiary structure. Also, the binding of the nucleotide to one form of the enzyme is promoted by sugar binding. These results are in accord with earlier solution studies on the ATPase activity of the enzyme in the absence of glucose. 2, record 5, English, - induced%2Dfit%20theory
Record 5, French
Record 5, Domaine(s)
- Sciences biologiques
Record 5, Main entry term, French
- théorie de l'ajustement induit
1, record 5, French, th%C3%A9orie%20de%20l%27ajustement%20induit
correct, feminine noun
Record 5, Abbreviations, French
Record 5, Synonyms, French
Record 5, Textual support, French
Record number: 5, Textual support number: 1 DEF
Théorie selon laquelle l'interaction enzyme-substrat peut causer un changement conformationnel dans la protéine enzymatique. 2, record 5, French, - th%C3%A9orie%20de%20l%27ajustement%20induit
Record number: 5, Textual support number: 1 CONT
La proposition faite par Koshland dès 1958 considère que le site actif, du fait d'un large déploiement d'amino-acides dans son environnement, possède une certaine flexibilité. De ce fait, au moment de l'entrée du substrat, le site actif réagit en s'adaptant minutieusement à la conformation du substrat et entreprend alors le processus de catalyse. Cette théorie de l'ajustement induit [...] a reçu des supports expérimentaux. Elle permet en outre de comprendre aisément la pénétration du substrat et le départ des produits en fin de réaction. 3, record 5, French, - th%C3%A9orie%20de%20l%27ajustement%20induit
Record 5, Spanish
Record 5, Textual support, Spanish
Record 6 - internal organization data 2010-09-29
Record 6, English
Record 6, Subject field(s)
- Biochemistry
- Food Industries
Record 6, Main entry term, English
- yeast enzyme
1, record 6, English, yeast%20enzyme
correct
Record 6, Abbreviations, English
Record 6, Synonyms, English
Record 6, Textual support, English
Record number: 6, Textual support number: 1 CONT
The source for the data in the Yeast ENZYME catalogue comes from recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology(IUBMB)(Enzyme Nomenclature, NC-IUBMB, Academic Press, New-York,(1992). 1, record 6, English, - yeast%20enzyme
Record 6, French
Record 6, Domaine(s)
- Biochimie
- Industrie de l'alimentation
Record 6, Main entry term, French
- enzyme de levure
1, record 6, French, enzyme%20de%20levure
correct, feminine noun
Record 6, Abbreviations, French
Record 6, Synonyms, French
Record 6, Textual support, French
Record number: 6, Textual support number: 1 CONT
Les radiaux libres conduisent par désamination de l'adénine à la formation d'hypoxanthine qui présente des propriétés miscodantes lors de la réplication. Une activité éliminant cette lésion avait été mise en évidence dans des extraits de cellules de mammifère et de E. coli. Nous avons montré que cette activité, décrite comme une entité enzymatique, était en réalité une des activités de la 3-méthyladénine ADN-glycosylase (la protéine ANPG humaine ou la protéine AlkA du colibacille), et que l'activité de cette protéine vis à vis de son substrat variait de manière très importante selon l'origine de la protéine : la protéine ANPG humaine est 100 fois plus active que l'enzyme de levure ou du colibacille. 1, record 6, French, - enzyme%20de%20levure
Record 6, Spanish
Record 6, Textual support, Spanish
Record 7 - internal organization data 2000-08-22
Record 7, English
Record 7, Subject field(s)
- Biotechnology
- Food Industries
Record 7, Main entry term, English
- Maxiren
1, record 7, English, Maxiren
correct, trademark
Record 7, Abbreviations, English
Record 7, Synonyms, English
Record 7, Textual support, English
Record number: 7, Textual support number: 1 CONT
Maxiren(Produced and marketed by Gist-Brocades International B. V.)-Maxiren is the biotechnology-produced version of calf rennet(chymosin) and is used as a milk-clotting enzyme in the production of cheese. It is produced by pure culture fermentation of the yeast Kluyveromyces lactis, modified to contain the calf gene encoding for chymosin. 1, record 7, English, - Maxiren
Record 7, French
Record 7, Domaine(s)
- Biotechnologie
- Industrie de l'alimentation
Record 7, Main entry term, French
- Maxiren
1, record 7, French, Maxiren
correct, trademark, feminine noun
Record 7, Abbreviations, French
Record 7, Synonyms, French
Record 7, Textual support, French
Record number: 7, Textual support number: 1 CONT
Une équipe de Madrid produit du fromage manchego au lait de brebis avec deux présures ou de la chymosine de Kluyveromyces lactis (Maxiren 15L - Gist Brocades). L'expérience est répétée 4 fois. L'enzyme Maxiren donne une coagulation, une protéolyse pendant l'affinage et des caractéristiques sensorielles identiques à celle des présures. 1, record 7, French, - Maxiren
Record 7, Key term(s)
- chymosine Maxiren
Record 7, Spanish
Record 7, Textual support, Spanish
Record 8 - internal organization data 1990-01-17
Record 8, English
Record 8, Subject field(s)
- Biological Sciences
Record 8, Main entry term, English
- cell structure
1, record 8, English, cell%20structure
correct
Record 8, Abbreviations, English
Record 8, Synonyms, English
Record 8, Textual support, English
Record number: 8, Textual support number: 1 CONT
Although Louis Pasteur recognized that fermentation is catalyzed by enzymes, he postulated in 1860 that they are inextricably linked with the structure and life of the yeast cell. It was therefore a major landmark in the history of enzyme research when, in 1897, Eduard Buchner succeeded in extracting from yeast cells the enzymes catalyzing alcoholic fermentation. This achievement clearly demonstrated that these important enzymes, which catalyze a major energy-yielding metabolic pathway, can function independently of cell structure. 1, record 8, English, - cell%20structure
Record 8, French
Record 8, Domaine(s)
- Sciences biologiques
Record 8, Main entry term, French
- structure cellulaire
1, record 8, French, structure%20cellulaire
correct, feminine noun
Record 8, Abbreviations, French
Record 8, Synonyms, French
Record 8, Textual support, French
Record number: 8, Textual support number: 1 CONT
La préparation et la purification, plus facile pour les enzymes solubles, se fait en utilisant des techniques de précipitation fractionnée. Pour les enzymes liées aux structures cellulaires, il faut broyer les tissus et isoler les éléments cellulaires contenant les enzymes que l'on désire extraire. La cristallisation (...) est une étape importante dans la purification. 1, record 8, French, - structure%20cellulaire
Record 8, Spanish
Record 8, Textual support, Spanish
Record 9 - internal organization data 1990-01-17
Record 9, English
Record 9, Subject field(s)
- Biological Sciences
Record 9, Main entry term, English
- cell-free metabolism
1, record 9, English, cell%2Dfree%20metabolism
correct
Record 9, Abbreviations, English
Record 9, Synonyms, English
Record 9, Textual support, English
Record number: 9, Textual support number: 1 CONT
One of Pasteur's central concepts, that each fermentation provides energy to the species conducting it, led to the accidental discovery of cell-free metabolism by Eduard Buchner of Germany in 1897. Buchner found that an extract of macerated yeast, freed of intact cells by filtration, retained the ability to convert sugar into alcohol... Later work showed that the biological conversion actually consisted of a series of simple chemical reactions, each catalyzed by a specific enzyme. 1, record 9, English, - cell%2Dfree%20metabolism
Record 9, French
Record 9, Domaine(s)
- Sciences biologiques
Record 9, Main entry term, French
- métabolisme acellulaire
1, record 9, French, m%C3%A9tabolisme%20acellulaire
correct, masculine noun
Record 9, Abbreviations, French
Record 9, Synonyms, French
Record 9, Textual support, French
Record number: 9, Textual support number: 1 CONT
L'un des concepts fondamentaux de Pasteur, à savoir que chaque fermentation fournit de l'énergie aux espèces qui se transforment, conduisit à la découverte accidentelle du métabolisme acellulaire par l'Allemand Eduard Buchner en 1897. Buchner découvrit qu'un extrait d'une macération de levures, débarrassé par filtration des cellules entières, transformait encore le sucre en alcool. (...) Des travaux ultérieurs montrèrent que la conversion biologique résultait d'une série de réactions chimiques simples, chacune d'elles étant catalysée par une enzyme spécifique. 1, record 9, French, - m%C3%A9tabolisme%20acellulaire
Record 9, Spanish
Record 9, Textual support, Spanish
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