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FRAGMENTATION REASSEMBLY [2 records]
Record 1 - internal organization data 1998-04-28
Record 1, English
Record 1, Subject field(s)
- Internet and Telematics
Record 1, Main entry term, English
- fragmentation and reassembly
1, record 1, English, fragmentation%20and%20reassembly
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record 1, French
Record 1, Domaine(s)
- Internet et télématique
Record 1, Main entry term, French
- fragmentation et réassemblage
1, record 1, French, fragmentation%20et%20r%C3%A9assemblage
correct
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 OBS
Dans Internet. 1, record 1, French, - fragmentation%20et%20r%C3%A9assemblage
Record 1, Spanish
Record 1, Textual support, Spanish
Record 2 - internal organization data 1996-07-12
Record 2, English
Record 2, Subject field(s)
- Genetics
Record 2, Main entry term, English
- DNA shuffling
1, record 2, English, DNA%20shuffling
correct
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, Textual support, English
Record number: 2, Textual support number: 1 DEF
A method for in vitro homologous recombination of pools of selected mutant genes by random fragmentation and polymerase chain reaction(PCR) reassembly. Computer simulations called genetic algorithms have demonstrated the importance of iterative homologous recombination for sequence evolution. 1, record 2, English, - DNA%20shuffling
Record number: 2, Textual support number: 1 CONT
As well as addressing the advantages (or lack thereof) of recombination, DNA shuffling provides a powerful tool for improving enzyme function in vitro. 2, record 2, English, - DNA%20shuffling
Record 2, French
Record 2, Domaine(s)
- Génétique
Record 2, Main entry term, French
- méthode du DNA shuffling
1, record 2, French, m%C3%A9thode%20du%20DNA%20shuffling
feminine noun
Record 2, Abbreviations, French
Record 2, Synonyms, French
Record 2, Textual support, French
Record number: 2, Textual support number: 1 CONT
La méthode la plus intéressante du point de vue de l'étude de l'évolution [moléculaire], est celle du DNA shuffling. Dans cette technique, l'ADN d'un gène est tronqué en fragments de 10 à 50 paires de bases, qui sont soumis à des cycles répétés de PCR sans amorce. Les produits PCR résultants sont sélectionnés pour leur expression phénotypique. Ce type d'expérience permet de simuler in vitro l'évolution moléculaire de séquences complexes. En mélangeant l'ADN de deux gênes, la création de chimères recombinantes peut également être envisagée. 1, record 2, French, - m%C3%A9thode%20du%20DNA%20shuffling
Record 2, Spanish
Record 2, Textual support, Spanish
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