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HETERODUPLEX [4 records]
Record 1 - internal organization data 2012-09-26
Record 1, English
Record 1, Subject field(s)
- Genetics
- Bioengineering
Record 1, Main entry term, English
- heteroduplex analysis
1, record 1, English, heteroduplex%20analysis
correct
Record 1, Abbreviations, English
- HA 1, record 1, English, HA
correct
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Heteroduplex is double-stranded DNA in which the two DNA strands do not show perfect base complementarity. When DNA is denatured, the two strands are separated. On renaturation or annealing, complementary DNA strands reassociate and form a homoduplex. However, when there is a mutation in one of the strands then a heteroduplex is formed. The electrophoretic mobility of heteroduplex in polyacrylamide gel is less than that of homoduplex, and they can be detected as an extra slow moving bands. Resolution is better in specially formulated gel matrices(Hydro-link, MDE, etc.). In fragments of under 200 bp insertions, deletions and most single base substitutions are detected. Heteroduplex analysis is mostly used combined with single confirmation analysis(SSCA) on a single gel. 1, record 1, English, - heteroduplex%20analysis
Record 1, French
Record 1, Domaine(s)
- Génétique
- Technique biologique
Record 1, Main entry term, French
- analyse d'hétéroduplex
1, record 1, French, analyse%20d%27h%C3%A9t%C3%A9roduplex
correct, feminine noun
Record 1, Abbreviations, French
- HA 2, record 1, French, HA
correct, feminine noun
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Nous présentons les avantages relatifs d'une stratégie de recherche spécifique des mutations qui introduisent une signal d'arrêt prématuré de la synthèse protéique fondée sur le test de troncation des protéines (PTT), par rapport aux techniques classiques de balayage d'un fragment d'ADN ou d'ARN telles que l'électrophorèse en gel de gradient dénaturant (DGGE), l'analyse d'hétéroduplex (HA), l'analyse de conformation de l'ADN en simple brin (SSCA) ou le clivage chimique ou enzymatique de mésappariements (CCM/EMC). 1, record 1, French, - analyse%20d%27h%C3%A9t%C3%A9roduplex
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Genética
- Bioingeniería
Record 1, Main entry term, Spanish
- análisis del heterodúplex
1, record 1, Spanish, an%C3%A1lisis%20del%20heterod%C3%BAplex
correct, masculine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 DEF
Empleo de la movilidad electroforética del ADN heterodúplex para estimar el grado de no homología entre las secuencias de las dos hebras. 1, record 1, Spanish, - an%C3%A1lisis%20del%20heterod%C3%BAplex
Record number: 1, Textual support number: 1 OBS
La movilidad en gel tiende a reducirse a medida que aumenta la divergencia entre secuencias, dado que el tamaño efectivo de un par de hebras totalmente complementarias es menor que el de una estructura donde la complementación es parcial. 1, record 1, Spanish, - an%C3%A1lisis%20del%20heterod%C3%BAplex
Record 2 - internal organization data 2012-03-16
Record 2, English
Record 2, Subject field(s)
- Genetics
Record 2, Main entry term, English
- heteroduplex
1, record 2, English, heteroduplex
correct
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, Textual support, English
Record number: 2, Textual support number: 1 DEF
A DNA double helix in which the complementary strands are derived from different molecules, so that there may be small regions of mismatching. 2, record 2, English, - heteroduplex
Record number: 2, Textual support number: 1 OBS
They occur naturally during genetic recombination between homologous chromosomes and can be produced artificially in vitro to estimate the degree of similarity between two molecules from different sources. 2, record 2, English, - heteroduplex
Record 2, French
Record 2, Domaine(s)
- Génétique
Record 2, Main entry term, French
- hétéroduplex
1, record 2, French, h%C3%A9t%C3%A9roduplex
correct, masculine noun, standardized
Record 2, Abbreviations, French
Record 2, Synonyms, French
- hétéroduplexe 2, record 2, French, h%C3%A9t%C3%A9roduplexe
correct, masculine noun
- hétéroduplex d'ADN 2, record 2, French, h%C3%A9t%C3%A9roduplex%20d%27ADN
correct, masculine noun
Record 2, Textual support, French
Record number: 2, Textual support number: 1 DEF
Molécule d'ADN partiellement alcaténaire dans laquelle les deux brins ne possèdent pas que des séquences de bases rigoureusement complémentaires. [Définition normalisée par l'AFNOR et reproduite avec son autorisation.] 3, record 2, French, - h%C3%A9t%C3%A9roduplex
Record number: 2, Textual support number: 1 OBS
hétéroduplex : s'emploie également comme adjectif. 4, record 2, French, - h%C3%A9t%C3%A9roduplex
Record number: 2, Textual support number: 2 OBS
hétéroduplex : terme normalisé par l'AFNOR. 5, record 2, French, - h%C3%A9t%C3%A9roduplex
Record 2, Spanish
Record 2, Campo(s) temático(s)
- Genética
Record 2, Main entry term, Spanish
- heterodúplex
1, record 2, Spanish, heterod%C3%BAplex
correct, masculine noun
Record 2, Abbreviations, Spanish
Record 2, Synonyms, Spanish
Record 2, Textual support, Spanish
Record 3 - internal organization data 2006-11-24
Record 3, English
Record 3, Subject field(s)
- Biotechnology
Record 3, Main entry term, English
- targeted induced local lesions in genome
1, record 3, English, targeted%20induced%20local%20lesions%20in%20genome
correct
Record 3, Abbreviations, English
- TILING 2, record 3, English, TILING
correct
Record 3, Synonyms, English
Record 3, Textual support, English
Record number: 3, Textual support number: 1 CONT
TILLING(Targeted Induced Local Lesions in Genomes) is a powerful technique that can identify a series of alleles or SNPs in a target gene by heteroduplex analysis. It can be adapted for use in a high-throughput facility, and has been successfully applied to all organisms tested in our laboratory. 3, record 3, English, - targeted%20induced%20local%20lesions%20in%20genome
Record 3, French
Record 3, Domaine(s)
- Biotechnologie
Record 3, Main entry term, French
- criblage de mutations locales dans le génome
1, record 3, French, criblage%20de%20mutations%20locales%20dans%20le%20g%C3%A9nome
proposal, masculine noun
Record 3, Abbreviations, French
Record 3, Synonyms, French
- TILING 2, record 3, French, TILING
see observation, masculine noun
Record 3, Textual support, French
Record number: 3, Textual support number: 1 CONT
Le «tiling» est une technique de génétique inverse [...] 2, record 3, French, - criblage%20de%20mutations%20locales%20dans%20le%20g%C3%A9nome
Record number: 3, Textual support number: 2 CONT
Le principe du «tiling» est d'introduire, au hasard, des mutations dans le génome de milliers de plantes (grâce à un produit chimique mutagène) et ensuite de repérer par, une approche moléculaire, les plantes renfermant les mutations recherchées dans un gène d'intérêt. 2, record 3, French, - criblage%20de%20mutations%20locales%20dans%20le%20g%C3%A9nome
Record number: 3, Textual support number: 1 OBS
TILING : Bien qu'il réfère à une expression anglaise, cet acronyme est largement utilisé en français. 1, record 3, French, - criblage%20de%20mutations%20locales%20dans%20le%20g%C3%A9nome
Record 3, Spanish
Record 3, Textual support, Spanish
Record 4 - internal organization data 1999-02-02
Record 4, English
Record 4, Subject field(s)
- Genetics
Record 4, Main entry term, English
- hybrid DNA
1, record 4, English, hybrid%20DNA
correct
Record 4, Abbreviations, English
Record 4, Synonyms, English
Record 4, Textual support, English
Record number: 4, Textual support number: 1 DEF
A double-strained DNA molecule with heteroduplex regions. 2, record 4, English, - hybrid%20DNA
Record number: 4, Textual support number: 1 CONT
It was also assumed that the reconstruction of DNA molecules in new ways, following breakage, took place not by an end-to-end association of broken chains but by a lateral association of complementary segments from homologous regions, to give hybrid DNA. 2, record 4, English, - hybrid%20DNA
Record 4, French
Record 4, Domaine(s)
- Génétique
Record 4, Main entry term, French
- ADN hybride
1, record 4, French, ADN%20hybride
correct, masculine noun
Record 4, Abbreviations, French
Record 4, Synonyms, French
Record 4, Textual support, French
Record number: 4, Textual support number: 1 DEF
Molécule d'ADN contenant une région hétéroduplex. 2, record 4, French, - ADN%20hybride
Record number: 4, Textual support number: 2 DEF
Molécule d'ADN composée de 2 brins d'origine distinctes. 3, record 4, French, - ADN%20hybride
Record number: 4, Textual support number: 1 CONT
Si l'on admet que le cross-over - source de recombinaison génétique réciproque - est ainsi associé à la formation d'un ADN hybride, un site mutationnel peut occasionnellement se situer à l'intérieur d'une région hybride, donnant ainsi naissance à un ADN hétérozygote. 4, record 4, French, - ADN%20hybride
Record 4, Spanish
Record 4, Textual support, Spanish
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