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HOMOPOLYMER TAILING TECHNIQUE [1 record]

Record 1 2012-08-31

English

Subject field(s)
  • Genetics
DEF

The addition of complementary bases to the ends of blunt-ended foreign and vector DNA sequences so that they may base-pair to form a recombinant DNA molecule.

OBS

For example, poly(dG) [polydeoxyguanylic acid] may be added to the ends of the foreign DNA while poly(dC) [polydeoxycytidylic acid] is added to ends of the vector DNA. The enzyme catalysing these transfer reactions is called terminal transferase.

French

Domaine(s)
  • Génétique
CONT

L'ADNc double brin [...] est inséré dans un vecteur, la plupart du temps pBR322 [plasmide de Bolivar et Rodriguez]. Deux types de méthodes sont utilisées pour ce faire : celle des adaptateurs ajoutés à chacune des extrémités des fragments, ou celle des séquences homopolymériques complémentaires.

Spanish

Campo(s) temático(s)
  • Genética
DEF

Adición in vitro de múltiples copias de un único nucleótido a los extremos 3'-hidroxilo de una molécula de ADN bicatenario por acción de la enzima transferasa terminal.

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