TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

ITC is the only technique that can simultaneously determine all binding parameters in a single experiment. Requiring no modification or immobilization of binding partners, ITC measures the affinity of binding partners in their native states. Measuring heat transfer during binding enables accurate determination of binding constants... reaction stoichiometry... enthalpy... and entropy...  2, record 1, English, - isothermal%20titration%20calorimetry

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

La calorimétrie de titrage isotherme (ITC) est une puissante technique d'analyse qui permet la caractérisation approfondie des événements de liaison moléculaire.  2, record 1, French, - calorim%C3%A9trie%20de%20titration%20isotherme

Record 1,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 DEF

Method of immobilization of enzymes by entrapment within the microcavities of synthetic fibres.  1, record 3, English, - fibre%20entrapment

Record number: 3, Textual support number: 1 CONT

The term entrapment has been used to cover a range of immobilization techniques. O’Driscoll has chosen to distinguish between those methods, where a gel or membrane is formed around an enzyme by polymerization and those methods where the enzyme is retained in a preformed polymer by subsequent formation of a cross-linked network. Microencapsulation and fiber entrapment are clearly other examples of this immobilization technique.  3, record 3, English, - fibre%20entrapment

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 CONT

En dehors de l'équipe de DINELLI, en Italie, qui a préparé de nombreuses enzymes immobilisées par inclusion dans des fibres d'acétate de cellulose, les enzymes immobilisées à structure non membranaire se présentent sous forme de particules de taille, de porosité et de propriétés mécaniques très variables. Ce sont les caractéristiques de ces particules qui conditionnent la mise en œuvre. Le choix du support pourra être ainsi différent selon que l'on envisage l'emploi de ces systèmes à petite échelle ou dans un but industriel.  1, record 3, French, - inclusion%20dans%20des%20fibres

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 DEF

Transfer of the carbamoyl of carbamoyl phosphate to an amino group with elimination of inorganic phosphate.  1, record 4, English, - carbamoylation

Record number: 4, Textual support number: 1 OBS

Many polymers containing isocyanate or isothiocyanate functional groups have been used as carbamylating or thiocarbamylating reagents, respectively, to effect coupling of proteins illustrating a class of immobilization technique. Polymers containing the isothiocyanate group have gained larger acceptance than those containing isocyanate owing to the higher stability of the former and the relative ease of its preparation. When isocyanates react in aqueous medium with proteins, the isocyanato groups are mainly hydrolyzed.  2, record 4, English, - carbamoylation

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Le traitement d'érythrocytes drépanocytaires par l'isocyanate provoque une carbamoylation des groupements aminés libres des valines N-terminales de l'hémoglobine S.  1, record 4, French, - carbamoylation

Record 4,  Textual support,  Spanish

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 DEF

Conversion of a soluble enzyme to a bound or insoluble form.  1, record 5, English, - enzyme%20immobilization

Record number: 5, Textual support number: 1 CONT

Methods for enzyme immobilization can be classified into three basic categories as follows. 1) Carrier-binding method: the binding of enzymes to water-insoluble carriers. 2) Cross-linking method: intermolecular cross-linking of enzymes by means of bifunctional or multifunctional reagents. 3) Entrapping method: incorporating enzymes into the lattice of a semipermeable gel or enclosing the enzymes in a semipermeable polymer membrane.  2, record 5, English, - enzyme%20immobilization

Record number: 5, Textual support number: 1 OBS

The technique has two advantages : expensive enzymes can be recovered and used again; the enzyme can be used in a variety of configurations of bioreactors that permit continuous operation. These include packed bed continuous-fed columns and expanded bed systems. A wide range of techniques are used for enzyme immobilization. These are similar in many ways to the techniques used to produce preparations of immobilized cells.  1, record 5, English, - enzyme%20immobilization

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 DEF

Technique de fixation des enzymes à un support soluble ou insoluble, de façon à les rendre très facilement récupérables en fin de réaction et être réemployées plusieurs fois et même fonctionner en continu dans des réacteurs. Il existe trois grands types d'immobilisation : l'inclusion, l'adsorption et la fixation par liaison covalente.  2, record 5, French, - immobilisation%20des%20enzymes

Record number: 5, Textual support number: 1 CONT

L'intérêt présenté par l'immobilisation des enzymes est double : - sur le plan conceptuel, il est possible de définir des modèles permettant de mieux appréhender le fonctionnement des enzymes membranaires in vivo : - sur le plan pratique, l'immobilisation des enzymes permet de mieux coordonner des actions séquentielles, de récupérer les enzymes après emploi, conduisant ainsi à la mise au point de ce que l'on appelle déjà des "réacteurs enzymatiques".  3, record 5, French, - immobilisation%20des%20enzymes

Record 5,  Textual support,  Spanish

Record 6,  Textual support,  English

Record number: 6, Textual support number: 1 DEF

Immobilization technique where a gel or membrane is formed around an enzyme by polymerization or where the enzyme is retained in a preformed polymer by subsequent formation of cross-linked network.  2, record 6, English, - entrapment

Record 6,  Textual support,  French

Record number: 6, Textual support number: 1 DEF

Procédé d'immobilisation dans le cas où l'enzyme est retenue dans un espace délimité par une membrane ou par un champ électromagnétique.  2, record 6, French, - pi%C3%A9geage

Record 6,  Textual support,  Spanish

Record 7,  Textual support,  English

Record number: 7, Textual support number: 1 OBS

JUDO. A category of ground techniques.  5, record 7, English, - osaekomi%2Dwaza

Record 7,  Textual support,  French

Record number: 7, Textual support number: 1 OBS

Technique/tactique. Généralement, la plupart des termes japonais en judo s'emploient au masculin, mais il y a des exceptions.  3, record 7, French, - osaekomi%2Dwaza

Record 7,  Textual support,  Spanish

Record 8,  Textual support,  English

Record number: 8, Textual support number: 1 CONT

The cost of most enzymes is such that they only become economic if they can be reused. This has led to the development of immobilization techniques, which involve converting the water-soluble enzyme protein into a solid form of catalyst, either by entrapping the enzyme within a cross-linked matrix of a water-insoluble polymer or by binding the enzyme ionically or covalently to the surface of a solid supporting material.  1, record 8, English, - immobilization%20technique

Record 8,  Textual support,  French

Record number: 8, Textual support number: 1 CONT

Le coût de la plupart des enzymes est tel que leur emploi ne devient rentable que s'il est possible de les réutiliser. C'est pourquoi on a mis au point des techniques d'immobilisation, qui consistent à transformer la protéine enzymatique soluble dans l'eau en un catalyseur à l'état solide, en piégeant l'enzyme dans une matrice réticulée formée d'un polymère insoluble dans l'eau ou en l'attachant par une liaison ionique ou covalente à la surface d'un support solide.  1, record 8, French, - technique%20d%27immobilisation

Record 8,  Textual support,  Spanish