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IMMOBILIZING MEMBRANE [6 records]

Record 1 2011-08-16

English

Subject field(s)
  • Scientific Research Equipment

French

Domaine(s)
  • Matériel et équipement (Recherche scientifique)

Spanish

Save record 1

Record 2 2011-08-09

English

Subject field(s)
  • Biological Sciences
CONT

Enzymes can be immobilized within microcapsules that have either a permanent or nonpermanent semipermeable membrane.... Nonpermanent membranes or "liquid-surfactant membranes"... refer to a water-immiscible phase composed of surfactants, "additives", and a hydrocarbon solvent that contain emulsion-size aqueous droplets of various reagents or catalysts.... The general procedure for immobilizing enzymes with liquid-surfactants membranes microcapsules is similar to that used for microencapsulation with permanent membranes. The aqueous enzyme-containing solution is emulsified with a surfactant to form the liquid membrane encapsulated enzyme. Once prepared, the microcapsules are transferred to an aqueous solution to which then can be added the substrate.

French

Domaine(s)
  • Sciences biologiques
CONT

Les microcapsules à membrane liquide [...] présentent l'intérêt de pouvoir être très facilement préparées et de permettre la récupération du matériau enzymatique par simple rupture. Ainsi lorsque l'on soumet à des ultrasons des agrégats obtenus par dispersion de phospholipides dans l'eau, on donne naissance à des sphères closes limitées par une double couche lipidique [...], les liposomes [...], emprisonnant des gouttelettes d'enzymes en solution aqueuse.

OBS

À l'inverse des autres techniques, la pénétration du substrat ne dépend pas alors de la taille des pores de la membrane mais de sa solubilité dans les constituants lipidiques.

Spanish

Save record 2

Record 3 1999-06-16

English

Subject field(s)
  • Bioengineering
  • Cytology
CONT

The availability of additional laser methods such as optical trapping(immobilizing and moving cells or organelles with a focused beam of light), optiporation(permeabilization of the cell membrane with light), optical fusion(inducting cells or vesicles for fuse with light), and optical ablation(optically incinerating cells or subcellular structures) provides an ever-increasing armory of optical tools for new and exciting investigations that depend upon macromanipulation of single cells and contaminant-free isolation of these cells from tissue slices or tissue culture.

French

Domaine(s)
  • Technique biologique
  • Cytologie

Spanish

Save record 3

Record 4 1999-06-16

English

Subject field(s)
  • Bioengineering
CONT

The availability of additional laser methods such as optical trapping(immobilizing and moving cells or organelles with a focused beam of light), optiporation(permeabilization of the cell membrane with light), optical fusion(inducting cells or vesicles for fuse with light), and optical ablation(optically incinerating cells or subcellular structures) provides an ever-increasing armory of optical tools for new and exciting investigations that depend upon macromanipulation of single cells and contaminant-free isolation of these cells from tissue slices or tissue culture.

French

Domaine(s)
  • Technique biologique
OBS

Perméabilisation de la membrane cellulaire à l'aide du rayon laser.

Spanish

Save record 4

Record 5 1999-06-16

English

Subject field(s)
  • Biochemistry
  • Bioengineering
CONT

The availability of additional laser methods such as optical trapping(immobilizing and moving cells or organelles with a focused beam of light), optiporation(permeabilization of the cell membrane with light), optical fusion(inducting cells or vesicles for fuse with light), and optical ablation(optically incinerating cells or subcellular structures) provides an ever-increasing armory of optical tools for new and exciting investigations that depend upon macromanipulation of single cells and contaminant-free isolation of these cells from tissue slices or tissue culture.

French

Domaine(s)
  • Biochimie
  • Technique biologique
CONT

Il est souvent délicat de contrôler avec précision la position de l'objet étudié en raison de sa taille et de sa fragilité. Cela devient plus délicat encore lorsqu'il s'agit d'approcher deux objets initialement séparés afin de les amener au contact. Pour réaliser de telles expériences, nous développons des outils spécifiques de manipulation : le piégeage optique par faisceaux laser et la micromanipulation mécanique. Le piégeage optique utilise la pression de radiation exercée par un ou deux faisceaux laser focalisés. On peut ainsi manipuler sans contact des objets de quelques microns. Nous utilisons cette technique pour mettre en contact une particule solide avec une vésicule géante.

Spanish

Save record 5

Record 6 1999-06-16

English

Subject field(s)
  • Bioengineering
  • Cytology
CONT

The availability of additional laser methods such as optical trapping(immobilizing and moving cells or organelles with a focused beam of light), optiporation(permeabilization of the cell membrane with light), optical fusion(inducting cells or vesicles for fuse with light), and optical ablation(optically incinerating cells or subcellular structures) provides an ever-increasing armory of optical tools for new and exciting investigations that depend upon macromanipulation of single cells and contaminant-free isolation of these cells from tissue slices or tissue culture.

French

Domaine(s)
  • Technique biologique
  • Cytologie
OBS

Ablation cellulaire par rayon laser.

Spanish

Save record 6

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