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INDIRECT CELL [5 records]

Record 1 2023-03-30

English

Subject field(s)
  • Bioengineering
  • Immunology
DEF

The use of antibodies, with a fluorescent marker dye attached, in order to detect whereabouts of specific antigens ... by formation of antibody-antigen complexes which show up on appropriate illumination.

OBS

The direct method utilizes a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. The indirect method permits detection of an antigen with a non-fluorochrome-conjugated antibody.

OBS

immunofluorescence method: This term appears under the entry "method" in the Stedman’s medical dictionary.

French

Domaine(s)
  • Technique biologique
  • Immunologie
DEF

Technique utilisée pour identifier microscopiquement des antigènes particuliers, dans des cellules ou des tissus, par la fixation d'anticorps couplés à un fluorochrome.

Spanish

Campo(s) temático(s)
  • Bioingeniería
  • Inmunología
DEF

Prueba de laboratorio que emplea reactivos fluorescentes para observar la presencia de un agente.

Save record 1

Record 2 2006-05-18

English

Subject field(s)
  • Vessels (Medicine)
  • Veterinary Medicine
CONT

When the antibodies are applied to fixed cells whose membranes have been made permeable to large molecules, they pass through the cell membrane and coat the microfilaments inside. A fluorescent label is then attached to the coated fibers, making them glow brightly when they are observed in the fluorescence microscope. This technique, called indirect immunofluorescence, is presently utilized by many investigators to make visible a variety of subcellular fibers.

French

Domaine(s)
  • Vaisseaux (Médecine)
  • Médecine vétérinaire
CONT

Technique caractérisée par l'utilisation d'un agent de marquage fluorescent sur des fibres cellulaires recouvertes d'anticorps.

CONT

Lorsque j'ai utilisé la technique de l'immunofluorescence indirecte pour mettre en évidence le réseau de microfilaments dans un couple de cellules-filles, j'ai constaté que l'organisation des fibres dans une cellule-fille était bien l'image dans un miroir de l'organisation observée dans l'autre.

Spanish

Campo(s) temático(s)
  • Redes vasculares (Medicina)
  • Medicina veterinaria
Save record 2

Record 3 2000-07-25

English

Subject field(s)
  • Psychology (General)
  • Psychometry and Psychotechnology
OBS

Network of cell bodies and fibers extending through the medulla, midbrain, hypothalamus, and thalamus forming an indirect sensory pathway to the cerebral cortex.

French

Domaine(s)
  • Psychologie (Généralités)
  • Psychométrie et psychotechnique

Spanish

Save record 3

Record 4 1997-01-20

English

Subject field(s)
  • Genetics
CONT

The residents will be exposed to the following areas : protein electrophoresis and immunofixation; direct and indirect immunofluorescence and enzyme immunohistology procedures; flow cytometry, including DNA ploidy/cell cycle analysis; anti-neutrophil cytoplasmic antibodies(ANCA) and anti-nuclear antibodies; enzyme immunoassay; in situ hybridization; and direct and indirect in situ PCR.

French

Domaine(s)
  • Génétique
CONT

Son principe est basé sur l'incorporation des nucléotides non marqués. La détection des produits amplifiés est réalisée par hybridation in situ classique grâce à des sondes radiomarquées ou couplées à un haptène.

OBS

Cette méthode de révélation est très spécifique et peut être comparée dans son principe au transfert de Southern (Southern blot) qui est, entre autres, employé pour confirmer les résultats obtenus par PCR liquide.

Spanish

Save record 4

Record 5 1982-04-16

English

Subject field(s)
  • Transportation Equipment (General)
  • Fuel Cells (Electr.)

French

Domaine(s)
  • Équipements de transport
  • Piles à combustible (Électricité)

Spanish

Save record 5

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