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INDIRECT FLUORESCENT ANTIBODY TECHNIQUE [2 records]

Filter results by subject field Alphabetical list of terms

Filter results by subject field

Alphabetical list of terms

Record 1 2006-05-18

English

Subject field(s)
  • Vessels (Medicine)
  • Veterinary Medicine
CONT

When the antibodies are applied to fixed cells whose membranes have been made permeable to large molecules, they pass through the cell membrane and coat the microfilaments inside. A fluorescent label is then attached to the coated fibers, making them glow brightly when they are observed in the fluorescence microscope. This technique, called indirect immunofluorescence, is presently utilized by many investigators to make visible a variety of subcellular fibers.

French

Domaine(s)
  • Vaisseaux (Médecine)
  • Médecine vétérinaire
CONT

Technique caractérisée par l'utilisation d'un agent de marquage fluorescent sur des fibres cellulaires recouvertes d'anticorps.

CONT

Lorsque j'ai utilisé la technique de l'immunofluorescence indirecte pour mettre en évidence le réseau de microfilaments dans un couple de cellules-filles, j'ai constaté que l'organisation des fibres dans une cellule-fille était bien l'image dans un miroir de l'organisation observée dans l'autre.

Spanish

Campo(s) temático(s)
  • Redes vasculares (Medicina)
  • Medicina veterinaria
Save record 1

Record 2 1993-03-03

English

Subject field(s)
  • Immunology
CONT

Fluorescent antibody technique : an immunofluorescence technique in which antigen in tissue sections is located by homologous antibody labeled with fluorochrome(the single-layer technique) or by treating the antigen with unlabeled antibody followed by a second layer of labeled antiglobulin which is reactive with the unlabeled antibody(double-layer technique). Variations includes direct, indirect, inhibition, and complement staining technique.

French

Domaine(s)
  • Immunologie
CONT

Technique (d'immunofluorescence) utilisant le complément : la réaction de l'anticorps spécifique avec le tissu entraîne la fixation du complément, secondairement ajouté à la préparation. Le complément est révélé par un antisérum marqué dirigé contre un facteur du complément (d'après S. Sell).

Spanish

Save record 2

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