TERMIUM Plus®
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INSIDE OUT PCR [1 record]
Record 1 - internal organization data 1996-07-12
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- inverted polymerase chain reaction 1, record 1, English, inverted%20polymerase%20chain%20reaction
Record 1, Abbreviations, English
- IPCR 2, record 1, English, IPCR
correct
Record 1, Synonyms, English
- inverse PCR 3, record 1, English, inverse%20PCR
correct
- inverse-PCR 3, record 1, English, inverse%2DPCR
correct
- inside out PCR 4, record 1, English, inside%20out%20PCR
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
Technique that can be employed in the determination of an unknown sequence that flank a region of known sequences. 4, record 1, English, - inverted%20polymerase%20chain%20reaction
Record number: 1, Textual support number: 1 OBS
The template target sequence is digested with a suitable restriction enzyme that cuts the template outside the known sequence but in the region of the unknown sequence. PCR is carried out using two primers which anneal to the ends of the known sequence but reversed in their direction with respect to their normal orientation in a normal PCR reaction. This leads to the amplification of the region of the unknown sequence. 4, record 1, English, - inverted%20polymerase%20chain%20reaction
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- PCR inverse
1, record 1, French, PCR%20inverse
correct, feminine noun
Record 1, Abbreviations, French
- IPCR 2, record 1, French, IPCR
correct, feminine noun
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Le principe même de la mutagénèse dirigée par IPCR nécessite la purification préalable des oligonucléotides utilisés. En effet, la présence de formes n-1, n-2 ou n-x même très minoritaire induirait la formation de mutations supplémentaires par délétion de une, deux ou x bases ors de 12 PCR. 1, record 1, French, - PCR%20inverse
Record number: 1, Textual support number: 2 CONT
Le produit final d'une PCR inverse (amorces tête-bêche) est phosphoryle ligué et transformé. 1, record 1, French, - PCR%20inverse
Record 1, Spanish
Record 1, Textual support, Spanish
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