TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

Technique for separating molecules based on their differential mobility in an electric field.  3, record 1, English, - electrophoresis

Record number: 1, Textual support number: 1 CONT

The principles of electrophoresis ... can be simply stated as the acceleration of charged particles in an electric field; this is opposed by friction due to passage through the surrounding medium such that the particles move at a constant speed proportional to their charge.  4, record 1, English, - electrophoresis

Record number: 1, Textual support number: 1 OBS

[Electrophoresis] is used routinely to separate the protein fractions of serum, urine, or cerebrospinal fluid; to separate serum lipoproteins; to separate the lactate dehydrogenase and creatine kinase isoenzymes; and to detect hemoglobin variants. It is also used in conjunction with immunodiffusion to separate proteins and identify them by antigenic differences.  3, record 1, English, - electrophoresis

Record number: 1, Textual support number: 1 PHR

Agarose gel, gel, paper, polyacrylamide gel electrophoresis.  5, record 1, English, - electrophoresis

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 DEF

Technique physicochimique permettant la séparation de diverses substances contenues dans un mélange, grâce à leur différence de mobilité sous l'influence d'un champ électrique (courant continu) [...]  3, record 1, French, - %C3%A9lectrophor%C3%A8se

Record number: 1, Textual support number: 1 OBS

[La] mobilité [des molécules], en présence d'une solution tampon de pH déterminé, est fonction, suivant le support utilisé, de la taille, de la forme, et surtout de la charge électrique de ces molécules.  3, record 1, French, - %C3%A9lectrophor%C3%A8se

Record number: 1, Textual support number: 1 PHR

Électrophorèse continue, sur gel, sur gel d'agarose, sur gel de polyacrylamide, sur papier, sur support.  4, record 1, French, - %C3%A9lectrophor%C3%A8se

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 DEF

Método de fraccionamiento molecular basado en diferencias de movilidad en un campo eléctrico que es función de la carga de la molécula.  2, record 1, Spanish, - electroforesis

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

A clonal derivation of passage viruses was requested since analysis of several clones from specific passage stocks showed them to be heterogenous in virulence as well as protein structure assessed by SDS page.  3, record 2, English, - sodium%20dodecyl%20sulphate%20polyacrylamide%20gel%20electrophoresis

Record number: 2, Textual support number: 1 OBS

SDS: sodium dodecyl sulfate.  4, record 2, English, - sodium%20dodecyl%20sulphate%20polyacrylamide%20gel%20electrophoresis

Record 2,  Textual support,  French

Record 2,  Textual support,  Spanish

Record number: 2, Textual support number: 1 DEF

Método muy extendido para separar por electroforesis las proteínas que contienen las muestras biológicas.  2, record 2, Spanish, - electroforesis%20en%20gel%20de%20poliacrilamida%20con%20dodecil%20sulfato%20s%C3%B3dico

Record number: 2, Textual support number: 1 OBS

El dodecil sulfato sódico confiere a la superficie de las proteínas o de los ácidos nucleicos una densidad de carga uniforme, de manera que sus velocidades de migración a través del gel quedan fundamentalmente determinadas por sus pesos moleculares.  2, record 2, Spanish, - electroforesis%20en%20gel%20de%20poliacrilamida%20con%20dodecil%20sulfato%20s%C3%B3dico

Record number: 2, Textual support number: 2 OBS

SDS-PAGE por sus siglas en inglés.  3, record 2, Spanish, - electroforesis%20en%20gel%20de%20poliacrilamida%20con%20dodecil%20sulfato%20s%C3%B3dico

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 OBS

A partially hydrolyzed polyacrylamide used as a mobility buffer.  2, record 3, English, - Dow%20700%26trade%3B

Record number: 3, Textual support number: 2 OBS

A trademark of The Dow Chemical Company.  2, record 3, English, - Dow%20700%26trade%3B

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 OBS

Dow 700^MC : Marque de commerce The Dow Chemical Company.  2, record 3, French, - Dow%20700

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 CONT

Heteroduplex is double-stranded DNA in which the two DNA strands do not show perfect base complementarity. When DNA is denatured, the two strands are separated. On renaturation or annealing, complementary DNA strands reassociate and form a homoduplex. However, when there is a mutation in one of the strands then a heteroduplex is formed. The electrophoretic mobility of heteroduplex in polyacrylamide gel is less than that of homoduplex, and they can be detected as an extra slow moving bands. Resolution is better in specially formulated gel matrices(Hydro-link, MDE, etc.). In fragments of under 200 bp insertions, deletions and most single base substitutions are detected. Heteroduplex analysis is mostly used combined with single confirmation analysis(SSCA) on a single gel.  1, record 4, English, - heteroduplex%20analysis

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Nous présentons les avantages relatifs d'une stratégie de recherche spécifique des mutations qui introduisent une signal d'arrêt prématuré de la synthèse protéique fondée sur le test de troncation des protéines (PTT), par rapport aux techniques classiques de balayage d'un fragment d'ADN ou d'ARN telles que l'électrophorèse en gel de gradient dénaturant (DGGE), l'analyse d'hétéroduplex (HA), l'analyse de conformation de l'ADN en simple brin (SSCA) ou le clivage chimique ou enzymatique de mésappariements (CCM/EMC).  1, record 4, French, - analyse%20d%27h%C3%A9t%C3%A9roduplex

Record 4,  Textual support,  Spanish

Record number: 4, Textual support number: 1 DEF

Empleo de la movilidad electroforética del ADN heterodúplex para estimar el grado de no homología entre las secuencias de las dos hebras.  1, record 4, Spanish, - an%C3%A1lisis%20del%20heterod%C3%BAplex

Record number: 4, Textual support number: 1 OBS

La movilidad en gel tiende a reducirse a medida que aumenta la divergencia entre secuencias, dado que el tamaño efectivo de un par de hebras totalmente complementarias es menor que el de una estructura donde la complementación es parcial.  1, record 4, Spanish, - an%C3%A1lisis%20del%20heterod%C3%BAplex

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 DEF

Ionic detergent used for denaturation of protein and in electrophoresis of protein subunits.  1, record 5, English, - sodium%20dodecyl%20sulphate

Record number: 5, Textual support number: 1 CONT

Detergent solubilization has been used with great success to study the major proteins in various membranes. Typically, the membranes are first heated to 100°C in a 1% solution of the strong detergent SDS(which disrupts all non-covalent protein-protein and protein-lipid interactions and completely unfolds the proteins), then layered on top of a polyacrylamide gel containing SDS, and lastly subjected to electrophoresis in a strong electric field for several hours. The amount of anionic SDS bound to protein is roughly equal to the mass of the polypeptide, with the result that the negative charge of the detergent overwhelms the intrinsic charge of the protein. Consequently, each individual protein migrates in the electric field at a rate determined by its molecular weight.  4, record 5, English, - sodium%20dodecyl%20sulphate

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 DEF

Détergent ionique employé comme agent dénaturant des protéines.  2, record 5, French, - dod%C3%A9cyl%20sulfate%20de%20sodium

Record number: 5, Textual support number: 1 CONT

Lors de l'électrophorèse en gel filtrant les protéines sont séparées selon leur taille moléculaire (ou encombrement stérique) et leur charge électrique. L'encombrement stérique d'une molécule dépend du poids moléculaire et de la configuration spatiale de la protéine. Pour mesurer le poids moléculaire d'une protéine, il faut donc éliminer l'influence de la charge et de la configuration spatiale. Un agent dénaturant, le dodécyl sulfate de sodium, permet d'obtenir ce résultat. Grâce à son affinité pour les protéines sur lesquelles il se fixe très solidement et en grande quantité, il leur confère une charge électrique constante par unité de surface et modifie considérablement leur configuration spatiale. C'est pourquoi le traitement par le SDS permet de mesurer directement le poids moléculaire d'une protéine après électrophorèse dans un gel de polyacrylamide.  4, record 5, French, - dod%C3%A9cyl%20sulfate%20de%20sodium

Record number: 5, Textual support number: 1 OBS

SDS est une abréviation de dodécyl sulfate de sodium.  2, record 5, French, - dod%C3%A9cyl%20sulfate%20de%20sodium

Record 5,  Textual support,  Spanish

Record number: 5, Textual support number: 1 DEF

Detergente utilizado para solubilizar proteínas y ADN de muestras biológicas.  1, record 5, Spanish, - dodecil%20sulfato%20s%C3%B3dico

Record number: 5, Textual support number: 1 OBS

Sólido de color blanco o amarillo. Soluble en agua. Poco tóxico.  2, record 5, Spanish, - dodecil%20sulfato%20s%C3%B3dico

Record 6,  Textual support,  English

Record number: 6, Textual support number: 1 DEF

A type of electrophoresis used to determine the size and composition of proteins.  4, record 6, English, - polyacrylamide%20gel%20electrophoresis

Record number: 6, Textual support number: 1 OBS

Electrophoresis carried out in acrylamide gel, often used for the study of individual proteins in serum, particularly genetic variants and enzymes. Three gel layers are constructed, differing in composition and pore size: separation gel, spacer gel, and bottom separation gel. The actual ion separation is based on the charge and molecular size and takes place in the bottom gel layer.  5, record 6, English, - polyacrylamide%20gel%20electrophoresis

Record 6,  Textual support,  French

Record number: 6, Textual support number: 1 CONT

La température de fusion d'un produit PCR (ADN double brin), c'est-à-dire la température moyenne de séparation des deux brins est fonction de sa séquence. Une mutation ponctuelle qui change donc la séquence entraîne une modification de la température de fusion. Cette modification est mise en évidence par électrophorèse sur gel de polyacrylamide en présence d'un gradient d'agent dénaturant.  1, record 6, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20de%20polyacrylamide

Record number: 6, Textual support number: 1 OBS

La plus fine des méthodes de séparation par simple électrophorèse sur support. Une solution d'acrylamide forme un gel par polymérisation. Les pores ont un diamètre de 50 [angstrom] environ. Les protéines à séparer sont déposées à une extrémité du gel dans lequel elles se déplacent sous l'action du champ électrique.  3, record 6, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20de%20polyacrylamide

Record 6,  Textual support,  Spanish

Record number: 6, Textual support number: 1 DEF

Método [...] para separar ácidos nucleicos y proteínas atendiendo a su tamaño molecular [...], se basa en la migración de moléculas cargadas eléctricamente a través de una matriz inerte (gel de poliacrilamida) como resultado de la aplicación de un campo eléctrico.  2, record 6, Spanish, - electroforesis%20en%20gel%20de%20poliacrilamida

Record number: 6, Textual support number: 1 OBS

PAGE por sus siglas en inglés.  3, record 6, Spanish, - electroforesis%20en%20gel%20de%20poliacrilamida

Record 7,  Textual support,  English

Record number: 7, Textual support number: 1 CONT

Differential Display(DD)... and a conceptually similar technique, arbitrarily primed PCR fingerprinting of RNA(APPCR) 19, both PCR amplify subsets of an mRNA population and separate the resulting cDNAs by denaturing polyacrylamide gel electrophoresis. Since the introduction of DD in 1992, over 100 reports regarding improvements and/or successful applications have been published. Although DD has the appearance of being technically easy, the road from "band on the gel" to a positive can be treacherous as outlined in a recent commentary. The primary criticisms are(1) a high false positive rate,(2) questioned ability of DD to identify both abundant and rare mRNAs,(3) coding regions of mRNAs are usually not cloned, and(4) verification process is time consuming and usually requires a fair amount of RNA.  5, record 7, English, - differential%20display

Record 7,  Textual support,  French

Record number: 7, Textual support number: 1 CONT

En 1992, deux chercheurs du Dana Farber Cancer Institute de Boston (États-Unis), P. Liang et AB Pardee, présentent une nouvelle méthode, le «differential display» (DD) - ou DDRT-PCR (DD reverse transcription polymerase chain reaction) qui permet une étude directe des modifications de l'expression génique [...] P. Liang et AB Pardee ont démontré que la combinaison d'amorces arbitraires et d'amorces spécifiques des ARNm permet de générer un set de fragments d'ADNc représentatifs des ARNm d'une cellule. Dans des conditions appropriées, le profil des fragments dérivés d'un type cellulaire est reproductible et peut être comparé à celui des fragments d'un autre type cellulaire. L'étude directe de l'expression différentielle des gènes devient possible.  2, record 7, French, - technique%20du%20DD

Record number: 7, Textual support number: 1 OBS

Outil d'expression génique.  1, record 7, French, - technique%20du%20DD

Record 7,  Textual support,  Spanish

Record number: 7, Textual support number: 1 DEF

Método para identificar ARNm que se encuentra a diferentes concentraciones en diferentes tejidos o en respuesta a tratamientos específicos.  1, record 7, Spanish, - visualizaci%C3%B3n%20diferencial

Record number: 7, Textual support number: 1 OBS

Los ARNm se convierte a ADNc; una determinada proporción de estos ADNc se amplifican por la reacción en cadena de la polimerasa y se separan por electroforesis.  1, record 7, Spanish, - visualizaci%C3%B3n%20diferencial

Record 8,  Textual support,  English

Record number: 8, Textual support number: 1 CONT

There are several types of electrophoresis, zone electrophoresis being one of the most common. In zone electrophoresis, proteins are supported on a solid so that, in addition to the electric migration forces, conventional chromatographic forces may enter into the separation efficiency. There are several types of zone electrophoresis according to the different supports. The common supports include starch gels, polyacrylamide gels, polyurethane foam, and paper.  3, record 8, English, - zone%20electrophoresis

Record number: 8, Textual support number: 2 CONT

zone electrophoresis of the tobacco mosaic virus.  2, record 8, English, - zone%20electrophoresis

Record 8,  Textual support,  French

Record number: 8, Textual support number: 1 DEF

Technique d'électrophorèse qui sépare les constituants d'un mélange sous forme de zones bien distinctes le long de l'axe de migration.  4, record 8, French, - %C3%A9lectrophor%C3%A8se%20sur%20gradient%20de%20densit%C3%A9

Record number: 8, Textual support number: 1 OBS

[Elle] se pratique soit dans un gel (polyacrylamide, amidon) contenu dans une colonne ou entre deux plaques de verre, soit dans un film (ruban de papier, acétate de cellulose) imprégné d'un tampon approprié.  5, record 8, French, - %C3%A9lectrophor%C3%A8se%20sur%20gradient%20de%20densit%C3%A9

Record 8,  Textual support,  Spanish

Record 9,  Textual support,  English

Record number: 9, Textual support number: 1 DEF

A gel ... made by cross-linking acrylamide with N’N’-methylene-bis-acrylamide.  3, record 9, English, - polyacrylamide%20gel

Record number: 9, Textual support number: 1 CONT

Polyacrylamide gel is a very suitable support [for enzyme immobilization] because, first it is chemically inert, second, the size of its pores can be varied by changing the concentration of the monomer and, third, at sufficiently high concentrations of the gel, the size of its pores is commensurate with the sizes of enzyme globules.  4, record 9, English, - polyacrylamide%20gel

Record number: 9, Textual support number: 1 OBS

Often referred to, incorrectly, as acrylamide gels.  3, record 9, English, - polyacrylamide%20gel

Record 9,  Textual support,  French

Record number: 9, Textual support number: 1 DEF

Gel dont le réseau est constitué d'un polymère préparé à partir de deux molécules de départ : l'acrylamide, petite molécule organique se terminant par un groupe amide (-CONH₂), et le N,N-méthylène bisacrylamide, l'agent de réticulation.  2, record 9, French, - gel%20de%20polyacrylamide

Record number: 9, Textual support number: 1 CONT

Pour la bioconversion [...], on s'oriente de plus en plus vers des enzymes isolées extraites des cellules. [...] L'enzyme est isolée par précipitation ou par purification sur résine. L'enzyme libre est immobilisée [...] dans un gel de polyacrylamide dans un réacteur et le substrat à traiter circule autour.  3, record 9, French, - gel%20de%20polyacrylamide

Record number: 9, Textual support number: 1 OBS

Nous retrouvons dans les méthodes d'immobilisation par liaison covalente des techniques d'inclusion dans des gels de polyacrylamide, techniques dans lesquelles se surajoute l'établissement de liaisons covalentes enzyme-gels.  4, record 9, French, - gel%20de%20polyacrylamide

Record 9,  Textual support,  Spanish

Record number: 9, Textual support number: 1 DEF

[...] gel [que] se forma por la polimerización vinílica del monómero acrilamida [...] y del monómero entrecruzador N, N'-metilen-bis-acrilamida, [sirve para separar moléculas biológicas].  2, record 9, Spanish, - gel%20de%20poliacrilamida

Record 10,  Textual support,  French

Record 10,  Textual support,  Spanish

Record 11,  Textual support,  French

Record 11,  Textual support,  Spanish

Record 12,  Textual support,  French

Record 12,  Textual support,  Spanish

Record 13,  Textual support,  English

Record number: 13, Textual support number: 1 DEF

A water soluble high polymer, used as a thickening agent, a suspending agent, an additive to adhesives, and a permissible food additive.  3, record 13, English, - polyacrylamide

Record number: 13, Textual support number: 1 OBS

Chemical formulas: CH=CHCONH₂ or (CH₂CHCONH₂)_x  4, record 13, English, - polyacrylamide

Record 13,  Textual support,  French

Record number: 13, Textual support number: 1 DEF

Nom générique des polymères et copolymères de l'acrylamide.  1, record 13, French, - polyacrylamide

Record number: 13, Textual support number: 1 OBS

Solubles dans l'eau, ils sont utilisés comme apprêts, épaississants, floculants, etc.  1, record 13, French, - polyacrylamide

Record number: 13, Textual support number: 2 OBS

Formules chimiques : CH=CHCONH₂ ou (CH₂CHCONH₂)_x  2, record 13, French, - polyacrylamide

Record 13,  Textual support,  Spanish

Record 14,  Textual support,  French

Record 14,  Textual support,  Spanish

Record 15,  Textual support,  English

Record number: 15, Textual support number: 1 CONT

Hydrophobic interaction chromatography... separates proteins on the basis of their relative hydrophobicities and uses insoluble supports, such as agarose or polyacrylamide, to which... alkyl or aryl... hydrophobic groups have been covalently attached. Liquid chromatography columns of such hydrophobic resins have been used in the purification of a number of integral membrane proteins. Proteins bound to the resin by means of hydrophobic interactions can then often be eluted sequentially, depending on the strength of this interaction, by gradual changes in hydrophobicity, ionic strength, pH, or temperature of the eluting buffer.  1, record 15, English, - hydrophobic%20group

Record 15,  Textual support,  French

Record number: 15, Textual support number: 1 DEF

Groupement moléculaire qui n'a aucune aptitude à retenir des molécules d'eau et qui est, au mieux, peu soluble dans l'eau.  2, record 15, French, - groupement%20hydrophobe

Record number: 15, Textual support number: 1 CONT

La chromatographie sur papier [...] repose sur les différences de solubilité relative des molécules dans un solvant aqueux et un solvant organique. [...] Plus un composé possède de groupements polaires [...] plus il est soluble dans l'eau, mais plus il possède de groupements hydrophobes [...] plus il est solubles dans les solvants organiques. On profite de la différence relative de solubilité de différents constituants d'un mélange [...]dans un liquide organique et dans un liquide aqueux pour les séparer.  3, record 15, French, - groupement%20hydrophobe

Record 15,  Textual support,  Spanish

Record 16,  Textual support,  English

Record number: 16, Textual support number: 1 DEF

Gel electrophoresis in which a discontinuity of pH or gel pore size is introduced near the point of origin producing a very thin starting zone and sharp separations.  1, record 16, English, - disc%20electrophoresis

Record number: 16, Textual support number: 1 CONT

The cross-linked polymer most commonly employed for enzyme entrapment is polyacrylamide gel, and it has been used for the immobilization of many enzymes. The procedure for the formation of polyacrylamide gel is identical to that employed for the preparation of gel commonly used for disc electrophoresis.  3, record 16, English, - disc%20electrophoresis

Record number: 16, Textual support number: 1 OBS

The term "disc" was originally proposed by the inventor primarily to express the idea of discontinuity, secondarily to reflect the discoid shape of the separate bands.  4, record 16, English, - disc%20electrophoresis

Record 16,  Textual support,  French

Record number: 16, Textual support number: 1 DEF

Méthode dérivant de l'électrophorèse de zones où le mélange protéique qui doit être analysé est soumis à un champ électrique dans un gel retardateur qui est séparé en deux portions différant par leur porosité et tamponnées à pH différents. Le mélange de protéines migre du gel le plus poreux vers le gel le moins poreux, ce processus s'accompagnant d'une modification de pH. Chaque espèce de protéines se concentre ainsi dans une zone plutôt étroite, en bandes minces, ce qui permet d'obtenir une très grande résolution, plus élevée que celle obtenue avec un tampon continu.  2, record 16, French, - %C3%A9lectrophor%C3%A8se%20discontinue

Record number: 16, Textual support number: 1 OBS

Disc electrophoresis et disc-electrophoreis sont des termes anglais souvent cités dans les ouvrages français. A déconseiller.  2, record 16, French, - %C3%A9lectrophor%C3%A8se%20discontinue

Record 16,  Textual support,  Spanish

Record 17,  Textual support,  English

Record number: 17, Textual support number: 1 DEF

A continuous string of flexible steel tubing, often hundreds or thousands of feet long, that is wound onto a reel, often dozens of feet in diameter.  4, record 17, English, - tube%20coil

Record number: 17, Textual support number: 1 CONT

(Polyacrylamide) polymers are susceptible to shear degradation when they pass through a throttling valve. The method... used for rate control is the insertion of a long coil of tubing of relatively small diameter.... The diameter of the tube coil is calculated based on the shear rate for the desired flow rate.  1, record 17, English, - tube%20coil

Record number: 17, Textual support number: 1 OBS

The reel is an integral part of the coiled tubing unit, which consists of several devices that ensure the tubing can be safely and efficiently inserted into the well from the surface.  4, record 17, English, - tube%20coil

Record 17,  Textual support,  French

Record number: 17, Textual support number: 1 DEF

Tube souple enroulé sur touret utilisé pour intervenir sur les puits sous pression.  1, record 17, French, - tube%20de%20production%20concentrique

Record 17,  Textual support,  Spanish

Record 18,  Textual support,  English

Record number: 18, Textual support number: 1 CONT

Fluorescence-based amplified fragment length polymorphism(fbAFLP) is a novel assay based on the fluorescent analysis of an amplified subset of restriction fragments. The fbAFLP assay involves the selective PCR [polymerase chain reaction] amplification of restriction fragments from a total digest of genomic DNA. The ligation of adapters with primer-specific sites coupled with primers containing selective nucleotides allowed the full potential of PCR to be realized while maintaining the advantages of restriction endonuclease analysis. Fluorescence-based fragment analysis with polyacrylamide gel electrophoresis provides the accurate band sizing required for homology assessment.  5, record 18, English, - fluorescent%20amplified%20fragment%20length%20polymorphism

Record 18,  Textual support,  French

Record number: 18, Textual support number: 1 OBS

L'utilisation du sigle anglais dans les textes français est fréquente. Toutefois, pour le moment, il ne semble y avoir aucun terme au long consacré en français.  2, record 18, French, - polymorphisme%20par%20fluorescence%20de%20longueur%20de%20fragments%20amplifi%C3%A9s

Record 18,  Textual support,  Spanish

Record 19,  Textual support,  English

Record number: 19, Textual support number: 1 CONT

Starch gel electrophoresis has been a popular technique, although it is now somewhat superseded by the use of polyacrylamide gels, which minimize convection and diffusion effects. A block or "plate" of starch gel is prepared, and the sample is applied in a narrow band(line) across the block about midway between the ends, which are contacted with electrodes through a connecting bridge. When current is passed through the cell, the different components of a mixture move with velocities that depend on their electric charges, their sizes, and their shapes. As electrophoresis proceeds, the negatively charged components migrate toward the anode and the positively charged components migrate toward the cathode. The result is a series of separated bands or lines of sample constituents, such as visualized by a stain.  2, record 19, English, - starch%20gel%20electrophoresis

Record number: 19, Textual support number: 1 OBS

Recently considerable progress has been made in assaying alleles, or variants, of some 15 genes that code for enzyme sysnthesis, which is readily detected by starch-gel electrophoresis.  3, record 19, English, - starch%20gel%20electrophoresis

Record 19,  Textual support,  French

Record number: 19, Textual support number: 1 OBS

On a fait de grands progrès dans la détection d'allèles, ou de variants d'une quinzaine de gènes qui codent pour des synthèses d'enzymes facilement décelées par électrophorèse sur gel d'amidon.  3, record 19, French, - %C3%A9lectrophor%C3%A8se%20de%20gel%20d%27amidon

Record 19,  Textual support,  Spanish

Record 20,  Textual support,  English

Record number: 20, Textual support number: 1 CONT

Polyacrylamide(PAM) is a man-made compound that when added to irrigation water in very small amounts can dramatically reduce soil erosion in row crops.... PAM and Surge Irrigation. Higher soil infiltration rates may increase leaching below the root zone due to PAM application. In turn, surge irrigation can help to reduce excessive soil infiltration. Therefore, PAM applications can be combined with surge irrigation to both reduce vertical infiltration and soil erosion substantially.  1, record 20, English, - vertical%20infiltration

Record 20,  Textual support,  French

Record number: 20, Textual support number: 1 CONT

Irrigation de surface : [...] L'irrigation par submersion se pratique en terrain plat dans des bassins, entourés de diguettes, que l'on remplit sur une hauteur correspondant à la dose d'arrosage. L'eau pénètre dans le sol par infiltration verticale. Le riz est la culture type ainsi irriguée, bien que sur la majeure partie du cycle végétatif l'arrosage ne soit pas discontinu mais permanent. Ce n'est que peu avant la récolte que les rizières sont mises à sec [...]  1, record 20, French, - infiltration%20verticale

Record 20,  Textual support,  Spanish

Record 21,  Textual support,  English

Record number: 21, Textual support number: 1 CONT

A direct method to visualise the aryl acylamidase activity on cholinesterases in polyacrylamide gels.... The o-nitroaniline liberated from o-nitroacetanilide by the action of aryl acylamidase activity on cholinesterases, in the presence of nitrous acid formed a diazonium compound.  2, record 21, English, - diazonium%20compound

Record number: 21, Textual support number: 2 CONT

Strictly speaking, diazo compounds are not dyes, but dye precursors. Some azo dyes are manufactured by coupling a phenol or naphthol to a diazonium compound.  3, record 21, English, - diazonium%20compound

Record 21,  Textual support,  French

Record number: 21, Textual support number: 1 OBS

diazonium : Nom générique des cations donnés par les diazoïques. Se dit aussi des sels correspondants. Ex. : chlorure de benzène-diazonium.  2, record 21, French, - compos%C3%A9%20diazonium

Record 21,  Textual support,  Spanish

Record 22,  Textual support,  English

Record number: 22, Textual support number: 1 CONT

Capillary gel electrophoresis(CGE) is the CE-analog of traditional slab-gel electrophoresis and is used for the size-based separation of biological macromolecules such as olgonucleotides, DNA restriction fragments and proteins. The separation is performed by filling the capillary with a sieve-like matrix, for example, cross-linked polyacrylamide, agarose or even solutions of linear polymer. The main advantages over slab-gel electrophoresis are a wider range of gel matrix and composition, online detection, improved quantification and automation.  1, record 22, English, - capillary%20gel%20electrophoresis

Record 22,  Textual support,  French

Record number: 22, Textual support number: 1 CONT

L'électrophorèse capillaire en gel est fondée sur l'utilisation de capillaires de silice remplis de gel de polyacrylamide ou d'agarose. Cette technique est dotée d'une résolution importante autorisant son application au séquençage. En effet, elle permet de distinguer de courts fragments d'ADN (inférieurs à 1000 bases) dont la taille ne varie que d'une base.  1, record 22, French, - %C3%A9lectrophor%C3%A8se%20capillaire%20en%20gel

Record 22,  Textual support,  Spanish

Record 23,  Textual support,  English

Record number: 23, Textual support number: 1 DEF

A technique in which charged complexes of protein with sodium dodecyl sulfate(SDS) are subjected to zone electrophoresis in a gel of uniform porosity [polyacrylamide gel] the rate of migration increasing as the molecular weight decreases.  1, record 23, English, - SDS%2Dgel%20electrophoresis

Record number: 23, Textual support number: 1 OBS

The relationship between the molecular weight of the protein and its mobility is linear, and can be used to estimate the protein’s molecular weight. SDS denatures the protein and dissociates it into its subunits to form long, rod-like SDS-polypeptide complexes.  1, record 23, English, - SDS%2Dgel%20electrophoresis

Record 23,  Textual support,  French

Record number: 23, Textual support number: 1 CONT

[Dans] l'électrophorèse sur gel de polyacrylamide en présence de SDS [une variante de l'électrophorèse de zones),] la migration des particules protéiques est directement proportionnelle à leur poids moléculaire. La concentration en acrylamide est calculée de façon à trier les protéines et peptides. On utilise une concentration unique ou un gradient continu selon le but et la résolution recherchés. Le sodium dodécylsulfate [(SDS) est] un détergent, qui dissocie [la protéine] en sous-unités et déroule complètement chaque chaîne polypeptidique pour former un complexe allongé entre le polypeptide et le SDS, [complexe] ayant la forme d'une baguette.  1, record 23, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20de%20polyacrylamide%20en%20pr%C3%A9sence%20de%20SDS

Record 23,  Textual support,  Spanish

Record 24,  Textual support,  English

Record number: 24, Textual support number: 1 CONT

Implantation of porcine bone morphogenetic protein(pBMP) in the muscle induces differentiation of mesenchymal-type cells and results in endochondral bone formation, pBMP was isolated from porcine demineralized bone matrix and purified by hydroxyapatite chromatography, Sephadex G75 gel filtration, preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE), preparative isoelectric focusing(IEF), and chromatofocusing fast protein liquid chromatography(FPLC).  1, record 24, English, - fast%20protein%20liquid%20chromatography

Record number: 24, Textual support number: 1 OBS

Term and context extracted from the PASCAL data base.  1, record 24, English, - fast%20protein%20liquid%20chromatography

Record 24,  Textual support,  French

Record number: 24, Textual support number: 1 OBS

Terme tiré de la liste française des descripteurs de l'article (base de données PASCAL).  1, record 24, French, - chromatographie%20FPLC

Record 24,  Textual support,  Spanish

Record 25,  Textual support,  English

Record number: 25, Textual support number: 1 DEF

Stain used to reveal the position of proteins of gels, following separation by electrophoresis or chromatography.  1, record 25, English, - Coomassie%20brilliant%20blue

Record number: 25, Textual support number: 1 CONT

What happens when a mixture of SDS-solubilized proteins is electrophoresed through a slab of polyacrylamide gel? Because they are associated with many negatively charged detergent molecules, each protein migrates toward the positive electrode when a voltage is applied. However, small proteins move much more readily through holes in the gel meshwork than large ones, and, as a result, a series of discrete protein bands is produced from a complex mixture, arranged in order of molecular weight. The major proteins present are readily detected by staining the gel with a dye such as Coomassie blue.  3, record 25, English, - Coomassie%20brilliant%20blue

Record 25,  Textual support,  French

Record number: 25, Textual support number: 1 CONT

La méthode habituelle pour séparer les polypeptides membranaires consiste à dissoudre les fantômes cellulaires dans le dodécyl sulfate de sodium (SDS) (...) et de les soumettre à l'électrophorèse en gel de polyacrylamide. Le gel peut être coloré pour détecter les protéines (bleu de Coomassie) ou les glucides (réactif PAS). (...) L'électrophorèse en gel de polyacrylamide (...) permet, par la position des bandes, d'évaluer le poids moléculaire des polypeptides et, par le profil de la surface, de déterminer leur masse relative.  1, record 25, French, - bleu%20de%20Coomassie

Record 25,  Textual support,  Spanish

Record 26,  Textual support,  English

Record number: 26, Textual support number: 1 CONT

The most suitable gel material requires both a relatively high concentration of the monomer(acrylamide) to give mechanical rigidity and a high concentration of crosslinking reagent(N, N. methylenebisacrylamide) to achieve the highest possible yield of immobilized enzyme activity.... The trapping efficiency of polyacrylamide gels of varying composition was examined by several investigators. Degani and Miron observed that the maximum yield of activity trapping(56%) occurred with cholinesterase at a crosslinking concentration of 5% and with a total and constant monomer concentration of 15%.  1, record 26, English, - activity%20trapping

Record 26,  Textual support,  French

Record 26,  Textual support,  Spanish

Record 27,  Textual support,  English

Record number: 27, Textual support number: 1 OBS

Subject-test CANMET-METALLURGY  2, record 27, English, - determination%20of%20polyacrylamide%20polymers

Record 27,  Textual support,  French

Record 27,  Textual support,  Spanish

Record 28,  Textual support,  English

Record number: 28, Textual support number: 1 CONT

The polymer is generally modified chemically by replacing some of the amide groups with a carboxyl group.... This process... is called hydrolysis.... Both hydrolyzed and unhydrolyzed polyacrylamide are highly polar due to the amide and carboxyl group  1, record 28, English, - hydrolyzed%20polyacrylamide

Record 28,  Textual support,  French

Record number: 28, Textual support number: 1 CONT

Ce modèle a été expérimentalement vérifié dans les conditions de la récupération assistée du pétrole, avec des polymères effectivement utilisés sur champ, polyacrylamides hydrolysés et polysaccharides Xanthane [...]  1, record 28, French, - polyacrylamide%20hydrolys%C3%A9

Record 28,  Textual support,  Spanish

Record 29,  Textual support,  English

Record number: 29, Textual support number: 1 CONT

The polymer is generally modified chemically by replacing some of the amide groups with a carboxyl group ... This process ... is called hydrolysis ... Both hydrolyzed and unhydrolyzed polyacrylamides are highly polar due to the amide and carboxyl groups.  1, record 29, English, - unhydrolyzed%20polyacrylamide

Record 29,  Textual support,  French

Record number: 29, Textual support number: 1 OBS

Par analogie avec «polyacrylamide hydrolysé».  1, record 29, French, - polyacrylamide%20non%20hydrolys%C3%A9

Record 29,  Textual support,  Spanish

Record 30,  Textual support,  English

Record number: 30, Textual support number: 1 CONT

... hydrolysis is accomplished by treating a polyacrylamide solution with a strong base, e. g. sodium hydroxide. The percentage of amide groups which have been changed to carboxyl groups is denoted as the per cent hydrolysis.  1, record 30, English, - per%20cent%20hydrolysis

Record 30,  Textual support,  French

Record number: 30, Textual support number: 1 OBS

(...) deux types de polymères (...) ont été utilisés 1) un échantillon de polyacrylamide hydrolysé (...) industriel en poudre (...) de degré d'hydrolyse proche de 30% (...)  2, record 30, French, - degr%C3%A9%20d%27hydrolyse%20en%20pourcentage

Record 30,  Textual support,  Spanish

Record 31,  Textual support,  English

Record number: 31, Textual support number: 1 CONT

LKB Macrophor DNA/RNA Sequencing System : For sequencing of DNA and RNA. New separation techniques involve the generation of a set of oligonucleotides having one end in common and differing in length in steps of one nucleotide, followed by electrophoretic separation of the oligonucleotides in a denaturing polyacrylamide gel.  1, record 31, English, - DNA%2FRNA%20sequencing%20system

Record 31,  Textual support,  French

Record number: 31, Textual support number: 1 CONT

Système séquenceur d'ADN/ARN Macrophor LKB : Des nouvelles techniques de séparation impliquent la génération d'un ensemble d'oligonucléotides ayant une extrémité en commun et différant en longueur en phases d'un nucléotide, suivi d'une séparation électrophorétique d'oligonucléotides dans un gel de polyacrylamide dénaturé.  1, record 31, French, - syst%C3%A8me%20s%C3%A9quenceur%20d%27ADN%2FARN

Record 31,  Textual support,  Spanish

Record 32,  Textual support,  English

Record number: 32, Textual support number: 1 CONT

Vertical Electrophoresis System. Dual slab capacity for up to 40 samples; interchangeable spacers accept 0. 75, 1. 5 or 3. 0 mm thick gels. A modular system for simple polyacrylamide, SDS or disc gel electrophoresis.  1, record 32, English, - vertical%20electrophoresis%20system

Record 32,  Textual support,  French

Record number: 32, Textual support number: 1 CONT

Système d'électrophorèse verticale. Plaque de gel grande surface permettant de traiter 40 échantillons; espaceurs interchangeables pour gels de 0.75, 1.5 ou 3 mm d'épaisseur. Système modulaire pour techniques d'électrophorèses en gels de polyacrylamide simple, SDS ou disque, en gradient de gel, en agarose ou en deux dimensions.  1, record 32, French, - syst%C3%A8me%20d%27%C3%A9lectrophor%C3%A8se%20verticale

Record 32,  Textual support,  Spanish

Record 33,  Textual support,  English

Record number: 33, Textual support number: 1 CONT

Complete Electrophoresis System.... Modular system based on the Multiphor II Electrophoresis Unit can adapt to perform high performance electrofocusing, analytical electrofocusing, preparative electrofocusing, preparative isotachophoresis, immuno and agarose electrophoresis, titration curves, gradient gels, SDS gels, polyacrylamide gel electrophoresis(PAGE) and two-dimensional electrophoresis.  1, record 33, English, - electrophoresis%20system

Record 33,  Textual support,  French

Record number: 33, Textual support number: 1 CONT

Système complet d'électrophorèse (...). Le système modulaire fondé sur l'appareil d'électrophorèse Multiphor II peut s'adapter pour effectuer l'électrofocalisation haute performance, l'électrofocalisation analytique, l'électrofocalisation préparative, l'isotachophorèse préparative, [etc].  1, record 33, French, - syst%C3%A8me%20d%27%C3%A9lectrophor%C3%A8se

Record 33,  Textual support,  Spanish

Record 34,  Textual support,  English

Record number: 34, Textual support number: 1 CONT

A igh torque motor makes it suitable for pumping high viscosity solutions or for forming [polyacrylamide gradient gels].  1, record 34, English, - polyacryaline%20gradient%20gel

Record 34,  Textual support,  French

Record number: 34, Textual support number: 1 OBS

Gel utilisé en biochimie à des gradients de concentration varié.  1, record 34, French, - gel%20de%20polyacrylamide%20%C3%A0%20gradient%20vari%C3%A9

Record 34,  Textual support,  Spanish