TERMIUM Plus®
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POOLED SAMPLE [2 records]
Record 1 - internal organization data 2021-03-17
Record 1, English
Record 1, Subject field(s)
- Scientific Research Methods
- Diagnostic Procedures (Medicine)
Record 1, Main entry term, English
- pooled sample
1, record 1, English, pooled%20sample
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
The process of getting a pooled sample is as follows. The same amount of sample is taken from "n" individuals and mixed(homogeneously). 2, record 1, English, - pooled%20sample
Record 1, French
Record 1, Domaine(s)
- Méthodes de recherche scientifique
- Méthodes diagnostiques (Médecine)
Record 1, Main entry term, French
- échantillon groupé
1, record 1, French, %C3%A9chantillon%20group%C3%A9
correct, masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- échantillon combiné 2, record 1, French, %C3%A9chantillon%20combin%C3%A9
correct, masculine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Chaque échantillon combiné est constitué du regroupement de 5 échantillons primaires de 200 g. 3, record 1, French, - %C3%A9chantillon%20group%C3%A9
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Métodos de investigación científica
- Procedimientos diagnósticos (Medicina)
Record 1, Main entry term, Spanish
- muestra mixta
1, record 1, Spanish, muestra%20mixta
feminine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
- muestra combinada 2, record 1, Spanish, muestra%20combinada
feminine noun
Record 1, Textual support, Spanish
Record 2 - internal organization data 1991-07-16
Record 2, English
Record 2, Subject field(s)
- Genetics
Record 2, Main entry term, English
- multiplex DNA sequencing
1, record 2, English, multiplex%20DNA%20sequencing
correct
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, Textual support, English
Record number: 2, Textual support number: 1 CONT
Multiplex DNA sequencing : The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N. Such a method is described. In order to separate the sequence information at the end of the processing, the DNA molecules of interest are ligated to a set of oligonucleotide "tags" at the beginning. The tagged DNA molecules are pooled, amplified, and chemically fragmented in 96-well plates. The resulting reaction products are fractionated by size on sequencing gels and transferred to nylon membranes. These membranes are then probed as many times as there are types of tags in the original pools, producing, in each cycle of probing, autoradiographs similar to those from standard DNA sequencing methods. 1, record 2, English, - multiplex%20DNA%20sequencing
Record 2, French
Record 2, Domaine(s)
- Génétique
Record 2, Main entry term, French
- méthode de séquençage multiplex 1, record 2, French, m%C3%A9thode%20de%20s%C3%A9quen%C3%A7age%20multiplex
Record 2, Abbreviations, French
Record 2, Synonyms, French
Record 2, Textual support, French
Record number: 2, Textual support number: 1 CONT
La méthode de séquençage multiplex : Plusieurs bibliothèques génomiques sont construites dans des vecteurs plasmidiques contenant chacun une séquence nucléotidique particulière, séquence cible spécifique. Des lots de 20 colonies provenant de chacune des 20 bibliothèques sont alors formés et les plasmides de chaque lot sont purifiés. La digestion enzymatique permet d'exciser les inserts contenant les fragments d'ADN génomique flanqués de leurs deux séquences cibles. Les opérations de séquençage sont alors effectuées selon la méthode chimique de Maxam et Gilbert, et les fragments obtenus sont séparés sur gel de polyacrylamide par électrophorèse. Une sonde correspondant à la séquence caractéristique du vecteur est utilisée pour révéler les fragments issus de ce clone. 1, record 2, French, - m%C3%A9thode%20de%20s%C3%A9quen%C3%A7age%20multiplex
Record 2, Spanish
Record 2, Textual support, Spanish
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