TERMIUM Plus®

The Government of Canada’s terminology and linguistic data bank.

SDS-PAGE [4 records]

Record 1 2012-10-23

English

Subject field(s)
  • Genetics
CONT

A clonal derivation of passage viruses was requested since analysis of several clones from specific passage stocks showed them to be heterogenous in virulence as well as protein structure assessed by SDS page.

OBS

SDS: sodium dodecyl sulfate.

French

Domaine(s)
  • Génétique

Spanish

Campo(s) temático(s)
  • Genética
DEF

Método muy extendido para separar por electroforesis las proteínas que contienen las muestras biológicas.

OBS

El dodecil sulfato sódico confiere a la superficie de las proteínas o de los ácidos nucleicos una densidad de carga uniforme, de manera que sus velocidades de migración a través del gel quedan fundamentalmente determinadas por sus pesos moleculares.

OBS

SDS-PAGE por sus siglas en inglés.

Save record 1

Record 2 1999-02-05

English

Subject field(s)
  • Genetics
CONT

Six mutants harboring intragenic suppressor mutation exhibit full restoration of export to the mutant RBP, on the other hand, two mutants harboring extragenic suppressor mutation show partial restoration of export. One intragenic suppressor showed the decreased rate of migration of the RBP during SDS-PAGE that may be caused by a second mutation in mature part of RBP. Mapping experiments showed that at least an extragenic suppressor phenotype is not the result of mutation in prlA(sec Y) gene.

French

Domaine(s)
  • Génétique
OBS

Mutation suppressive : Mutation qui, associe à une première mutation, en compense les effets phénotypiques.

Spanish

Save record 2

Record 3 1991-06-06

English

Subject field(s)
  • Electrokinetics in Liquids and Solids
DEF

A technique in which charged complexes of protein with sodium dodecyl sulfate (SDS) are subjected to zone electrophoresis in a gel of uniform porosity [polyacrylamide gel] the rate of migration increasing as the molecular weight decreases.

OBS

The relationship between the molecular weight of the protein and its mobility is linear, and can be used to estimate the protein’s molecular weight. SDS denatures the protein and dissociates it into its subunits to form long, rod-like SDS-polypeptide complexes.

French

Domaine(s)
  • Effets électrocinétiques dans les liquides et les solides
CONT

[Dans] l'électrophorèse sur gel de polyacrylamide en présence de SDS [une variante de l'électrophorèse de zones),] la migration des particules protéiques est directement proportionnelle à leur poids moléculaire. La concentration en acrylamide est calculée de façon à trier les protéines et peptides. On utilise une concentration unique ou un gradient continu selon le but et la résolution recherchés. Le sodium dodécylsulfate [(SDS) est] un détergent, qui dissocie [la protéine] en sous-unités et déroule complètement chaque chaîne polypeptidique pour former un complexe allongé entre le polypeptide et le SDS, [complexe] ayant la forme d'une baguette.

Spanish

Save record 3

Record 4 1991-01-31

English

Subject field(s)
  • Bioengineering
CONT

Implantation of porcine bone morphogenetic protein(pBMP) in the muscle induces differentiation of mesenchymal-type cells and results in endochondral bone formation, pBMP was isolated from porcine demineralized bone matrix and purified by hydroxyapatite chromatography, Sephadex G75 gel filtration, preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE), preparative isoelectric focusing(IEF), and chromatofocusing fast protein liquid chromatography(FPLC).

OBS

Term and context extracted from the PASCAL data base.

French

Domaine(s)
  • Technique biologique
OBS

Terme tiré de la liste française des descripteurs de l'article (base de données PASCAL).

Spanish

Save record 4

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