TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

Examination of whole cell lipid profiles for PM1 [Methylibium petroleiphilum strain] and YM1 [Bacillus sp. strain] indicates that they are not identical strains. Thermal gradient gel electrophoresis(TGGE) analysis of 16S rRNA gene fragments from the two strains, however, showed no difference between them. 16S rDNA sequence analysis of strains PM1 and YM1 revealed that they are both most closely related to the genus Sphingomonas.  2, record 1, English, - thermal%20gradient%20gel%20electrophoresis

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

L'analyse de fragments obtenus après digestion de l'ADN ribosomal amplifié par des enzymes de restriction (ARDRA, amplified ribosomal DNA restriction analysis), la séparation des fragments amplifiés par électrophorèse sur gel avec gradient de dénaturation (DGGE, TGGE: thermal gradient gel electrophoresis) permettent de caractériser les communautés microbiennes par leur signature moléculaire.  3, record 1, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20en%20gradient%20de%20temp%C3%A9rature

Record number: 1, Textual support number: 1 OBS

TGGE est le sigle anglais.  4, record 1, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20en%20gradient%20de%20temp%C3%A9rature

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 DEF

Método para separar fragmentos de ADN según su movilidad bajo condiciones que favorecen progresivamente la desnaturalización por calor.  1, record 1, Spanish, - electroforesis%20en%20gel%20de%20gradiente%20t%C3%A9rmico

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

PCR consists in the selective amplification of a given DNA sequence from a pathogen's genome. In qualitative PCR, the presence of an amplification product is checked by gel electrophoresis...  3, record 2, English, - qualitative%20PCR

Record number: 2, Textual support number: 2 CONT

When PCR is used only for detecting a specific DNA segment, the method is referred to as qualitative PCR. ... Qualitative PCR is an extremely sensitive method which is theoretically able to detect a single DNA molecule in a sample solution. In many cases specific genes are copied in this way, e.g. in order to identify pathological changes. ... Qualitative PCR is also used around the world in forensic medicine to identify individuals. ... only qualitative PCR can determine whether an infection has been eradicated, whether it is chronic (and might therefore progress unnoticed) and whether the individual has been reinfected with a different but related pathogen.  4, record 2, English, - qualitative%20PCR

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 DEF

Examen de biologie moléculaire, réalisé dans des laboratoires spécialisés, permettant, grâce à une technique d'amplification, la détection des brins d'ARN dans le sérum.  3, record 2, French, - PCR%20qualitative

Record number: 2, Textual support number: 1 CONT

La PCR qualitative est une technique très sensible c'est-à-dire qu'elle détecte le virus même en très petite quantité dans le sang. [...] Elle est utile lors du diagnostic d'hépatite virale C. Par exemple, si on trouve des anticorps anti-VHC dans le sang d'un individu, [le] médecin fait [...] réaliser une PCR qualitative qui confirme ou non la persistance du virus dans l'organisme. [La PCR qualitative] est [aussi] utile lors de la surveillance d'un traitement. Par exemple, le médecin prescrit une PCR qualitative à la fin du traitement de l'hépatite puis 24 semaines après pour savoir si le virus est encore présent ou non, c'est-à-dire si le traitement a été efficace ou non.  4, record 2, French, - PCR%20qualitative

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 CONT

Three families of repetitive sequences have been studied in most detail, including the 35-40 bp [base pair] repetitive extragenic palindromic(REP) sequence, the 124-127 bp enterobacterial repetitive intergenic consensus(ERIC) sequence, and the 154 bp BOX element. These sequences appear to be located in distinct, intergenic positions all around the chromosome. The repetitive elements may be present in both orientations on the chromosome, and PCR [polymerase chain reaction] primers have been designed to "read outward" from the inverted repeats in REP and ERIC, and from the boxA subunit of BOX. The use of the above primer(s) and PCR leads to the selective amplification of distinct genomic regions located between REP, ERIC or BOX sequences. The corresponding protocols are referred to as REP-PCR, ERIC-PCR and BOX-PCR, respectively, and rep-PCR collectively. Amplified bands are size fractionated through a gel matrix to yield fingerprint patterns resembling "bar codes", analogous to UPC codes used in grocery stores, and function as a signature for specific bacterial strains.  3, record 3, English, - BOX%2DPCR

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 DEF

Technique d'amplification génique effectuée au moyen d'amorces de type BOX afin d'établir l'empreinte génétique d'un microorganisme.  2, record 3, French, - BOX%2DPCR

Record number: 3, Textual support number: 1 OBS

L'utilisation de l'article semble rare. Par exemple, on parlerait plutôt «d'empreinte génétique par BOX-PCR» que «d'empreinte génétique par la BOX-PCR».  2, record 3, French, - BOX%2DPCR

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Ces gels dits «de séquence» sont capables de séparer des fragments d'ADN simple brin de taille inférieure à 600 bases, qui diffèrent les uns des autres d'une seule base . La détermination de la séquence d'un fragment donné consiste à générer tous les fragments se terminant successivement par T.  1, record 4, French, - gel%20de%20s%C3%A9quence

Record 4,  Textual support,  Spanish