TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

The transfer of genetic material from one organism to another.  2, record 1, English, - transgenesis

Record number: 1, Textual support number: 1 CONT

We determined the expression pattern of the tissue inhibitor of metalloproteinase(TIMP) in the development of the mouse embryo using in situ hybridization and transgenesis. To define the sequences regulating TIMP expression, we generated transgenic mice that expressed the Escherichia coli beta-galactosidase gene under control of a 5’ region of the mouse TIMP gene containing-2158 to-58 by upstream of the initiator ATG.  3, record 1, English, - transgenesis

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 DEF

Transfert de matériel génétique d'un organisme à un autre.  2, record 1, French, - transg%C3%A9n%C3%A8se

Record number: 1, Textual support number: 1 CONT

L'introduction d'un gène étranger dans le patrimoine héréditaire d'un individu définit la transgénèse. Différentes techniques sont utilisées chez les mammifères pour y parvenir. Les deux premières ne permettent pas, à elles seules, de choisir l'endroit où le transgène s'intégrera. Il s'agit : (1) de la micro-injection dans l'œuf, qui permet d'introduire de grands fragments d'ADN exogène; (2) des rétrovirus recombinants défectifs qui permettent d'intégrer avec une fréquence élevée des fragments d'ADN dont la taille est cependant limitée par la place disponible dans la capside du rétrovirus.  3, record 1, French, - transg%C3%A9n%C3%A8se

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 DEF

Transferencia de un gen (que se convierte en transgén) a un organismo receptor (llamado transgénico), que generalmente puede transmitirlo a su descendencia.  2, record 1, Spanish, - transg%C3%A9nesis

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

A physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin.  4, record 2, English, - fluorescence%20in%20situ%20hybridization

Record number: 2, Textual support number: 1 CONT

Until recently, even the best chromosomal maps could be used to locate a DNA [deoxyribonucleic acid] fragment only to a region of about 10 Mb, the size of a typical band seen on a chromosome. Improvements in fluorescence in situ hybridization(FISH) methods allow orientation of DNA sequences that lie as close as 2 to 5 Mb.  2, record 2, English, - fluorescence%20in%20situ%20hybridization

Record number: 2, Textual support number: 2 CONT

Fluorescent in situ hybridization on interphase nuclei prepared from normal male fibroblast cultures gave a distance between D9S12 and D9S180 of about one Mb.  3, record 2, English, - fluorescence%20in%20situ%20hybridization

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

Le développement des techniques d'hybridation in situ en fluorescence et la mise au point de systèmes d'imagerie dont les performances répondent aux exigences des cytogénéticiens permettent aujourd'hui la mise en œuvre de méthodes analytiques puissantes, clés de l'investigation efficace du génome et de ses anomalies.  2, record 2, French, - hybridation%20in%20situ%20en%20fluorescence

Record 2,  Textual support,  Spanish

Record number: 2, Textual support number: 1 DEF

[Técnica] que utiliza sondas de ADN [ácido desoxirribonucleico] marcadas con fluorescencia para detectar o confirmar anomalías génicas o cromosómicas que generalmente están más allá del poder de resolución de la citogenética de rutina.  1, record 2, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record number: 2, Textual support number: 1 CONT

Fluorescence in situ hybridization (FISH) o hibridación fluorescente in situ es una técnica que combina citogenética clásica con biología molecular. Con esta asociación se logra obtener la información genética manteniendo la estructura de las células o tejidos estudiados. [...] FISH es una herramienta importante en investigación y se incrementa su uso en diagnóstico humano. [...] ejemplos de su aplicación [...]: genética pre y postnatal; diagnóstico, seguimiento y/o pronóstico en cáncer; determinaciones de anomalías en embriones de preimplantación; microbiología; radiobiología; medio ambiente y evolución.  2, record 2, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record number: 2, Textual support number: 1 OBS

FISH por su sigla en inglés.  3, record 2, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 CONT

One of the important modifications of the ISH [in situ hybridization] technique is genomic in situ hybridization(GISH). GISH is a genomic painting technique which allows parental genomes in interspecific hybrids to be distinguished.  2, record 3, English, - genomic%20in%20situ%20hybridization

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 CONT

Nous avons également analysé leur comportement méiotique et leur composition chromosomique, au moyen de techniques cytogénétiques et d’analyses GISH (hybridation génomique in situ).  2, record 3, French, - hybridation%20g%C3%A9nomique%20in%20situ

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 DEF

The process by which a chromosome or plasmid map is constructed.  3, record 4, English, - genetic%20mapping

Record number: 4, Textual support number: 1 OBS

It involves a variety of techniques, such as cytologic deletion, complementation, and linkage mapping; in situ hybridization; pedigree analysis; recombinant DNA studies and somatic cell genetics.  3, record 4, English, - genetic%20mapping

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 DEF

Ensemble des méthodes permettant de déterminer les positions relatives des gènes, locus, sites de mutation, de restriction, etc., sur les chromosomes.  4, record 4, French, - cartographie%20g%C3%A9n%C3%A9tique

Record 4,  Textual support,  Spanish

Record number: 4, Textual support number: 1 DEF

Construcción de un mapa genético total (de todo el genoma) o parcial (alrededor de un gen).  1, record 4, Spanish, - cartograf%C3%ADa%20gen%C3%A9tica

Record number: 4, Textual support number: 1 OBS

De manera más general, determinación de la situación de un locus (gen o marcador genético) en un cromosoma.  1, record 4, Spanish, - cartograf%C3%ADa%20gen%C3%A9tica

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 DEF

A method of localizing and detecting specific mRNA [messenger RNA] sequences in morphologically preserved tissues sections or cell preparations by hybridizing the complementary strand of a nucleotide probe to the sequence of interest.  2, record 5, English, - in%20situ%20hybridization

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 DEF

Hybridation d'une sonde d'ADN ou d'ARN spécifique marquée avec l'ARN ou l'ADN cellulaire, sur une coupe de tissu. [Définition normalisée par l'AFNOR et reproduite avec son autorisation.]  2, record 5, French, - hybridation%20in%20situ

Record number: 5, Textual support number: 1 CONT

L'hybridation in situ permet l'identification de séquences d'acides nucléiques (ARN ou ADN) exogènes ou endogènes directement au sein d'une cellule ou d'un tissu. Son principe repose sur l'appariement direct (par des liaisons hydrogène) d'une sonde dont la séquence est complémentaire de celle des acides nucléiques à caractériser.  3, record 5, French, - hybridation%20in%20situ

Record number: 5, Textual support number: 1 OBS

hybridation in situ : terme normalisé par l'AFNOR.  4, record 5, French, - hybridation%20in%20situ

Record 5,  Textual support,  Spanish

Record 6,  Textual support,  English

Record number: 6, Textual support number: 1 CONT

The training in clinical cytogenetics includes extensive laboratory experience, gaining independent expertise in both long-and short-term cell culture, the recognition and collection of mitotic cells and slide preparation, and a variety of banding techniques including, but not limited to, G-, Q-, C-, and NOR-banding, and most recently fluorescent in situ hybridization.  2, record 6, English, - NOR%20band

Record 6,  Textual support,  French

Record number: 6, Textual support number: 1 CONT

Les bandes NOR, qui par imprégnation à l'argent, révèlent les organisateurs nucléolaires. La topographie des bandes et la description des anomalies fait l'objet d'une convention internationale appliquée aux cas de nombreuses espèces dont l'homme.  1, record 6, French, - bande%20NOR

Record 6,  Textual support,  Spanish

Record 7,  Textual support,  English

Record 7,  Textual support,  French

Record number: 7, Textual support number: 1 OBS

Texte sur le virus du papillome humain.  1, record 7, French, - hybridation%20de%20l%27ADN%20in%20situ%20sur%20filtre

Record 7,  Textual support,  Spanish

Record 8,  Textual support,  English

Record number: 8, Textual support number: 1 CONT

Autosyndetic pairing was distinguished from allosyndetic pairing in the hybrids by in situ hybridization(GISH).  2, record 8, English, - allosyndetic

Record 8,  Textual support,  French

Record number: 8, Textual support number: 1 DEF

Qui a trait à l'allosyndèse.  1, record 8, French, - allosynd%C3%A9tique

Record number: 8, Textual support number: 1 OBS

allosyndèse : À la méiose d'un hybride entre deux espèces polyploïdes, appariement entre les chromosomes de ces deux espèces.  1, record 8, French, - allosynd%C3%A9tique

Record 8,  Textual support,  Spanish

Record 9,  Textual support,  English

Record number: 9, Textual support number: 1 CONT

Chromogranin B(secretogranin I or Scg-1) is a tyrosine-sulfated secretory protein found in a wide variety of peptidergic endocrine cells. Benedum et al.(1987) isolated a 2. 5-kb cDNA clone from a cDNA library of human pheochromocytoma. Chromogranin B is a 657 amino acid long polypeptide of 76 kilodaltons and is preceded by a cleaved end-terminal signal peptide of 20 residues. Chromogranin B was assigned to chromosome 20 by Craig et al.(1986). Craig et al.(1987) showed by in situ hybridization that the CHGB locus is on 20pter-p12. Jenkins et al.(1991) mapped the murine gene, symbolized Scg-1, to chromosome 2 by in situ hybridization and by interspecific backcross analysis.  2, record 9, English, - chromogranin%20B

Record 9,  Textual support,  French

Record number: 9, Textual support number: 1 CONT

Le peptide correspondant à la séquence C-terminale de la chromogranine B a une activité lytique sélective contre la bactérie Micrococcus luteus à des concentrations infimes [...]  2, record 9, French, - chromogranine%20B

Record 9,  Textual support,  Spanish

Record 10,  Textual support,  English

Record number: 10, Textual support number: 1 CONT

The LBNL/UCSF Resource for Molecular Cytogenetics has been created to facilitate the application of molecular cytogenetics in clinical and biological studies. Work is being pursued in three areas : Development and application of improved hybridization technology, selection of probes optimized for use in fluorescence in situ hybridization(FISH), and development of digital imaging microscopy.  1, record 10, English, - molecular%20cytogenetics

Record number: 10, Textual support number: 1 OBS

Resource for Molecular Cytogenetics Cancer Center, University of California, San Francisco Lawrence Berkeley National Laboratory, Berkeley California.  2, record 10, English, - molecular%20cytogenetics

Record 10,  Textual support,  French

Record number: 10, Textual support number: 1 CONT

Cependant deux nouvelles techniques de cytogénétique moléculaire utilisant la FISH ont été développées au cours des cinq dernières années : l'hybridation génomique comparative et le caryotype spectral.  1, record 10, French, - cytog%C3%A9n%C3%A9tique%20mol%C3%A9culaire

Record 10,  Textual support,  Spanish

Record 11,  Textual support,  English

Record number: 11, Textual support number: 1 CONT

Primed in situ labeling(PRINS) is a fast alternative to traditional in situ hybridization, requiring only a tew handling steps(1, 2). In the PRINS reaction, an unlabeled sequence-specific oligonucleotide primer or short DNA fragment is hybridized(annealed) to denatures chromosomal DNA and extended in situ in the presence of hapten-labeled nucleotide triphos phates by a suitable DNA polymerase(3). The technique is especially suited for the rapid identification of chromo somes with oligonucleotide primers specific for individual chromosomal sequences and chromosomal elements like centromer or satellite sequences.  2, record 11, English, - primed%20in%20situ%20labeling%20of%20nucleic%20acids

Record 11,  Textual support,  French

Record number: 11, Textual support number: 1 CONT

La technique PRINS (primed in situ DNA synthesis), encore dénommée amorçage in situ, permet de localiser au niveau chromosomique des séquences répétées et présentes en de nombreuses copies. Elle nécessite une amorce nucléotidique capable de s'hybrider à la séquence d'ADN dénaturé. L'hybridation de l'amorce est suivie d'une extension in situ par incorporation, en présence de la Taq polymérase [...]  1, record 11, French, - technique%20PRINS

Record 11,  Textual support,  Spanish

Record 12,  Textual support,  English

Record number: 12, Textual support number: 1 CONT

The residents will be exposed to the following areas : protein electrophoresis and immunofixation; direct and indirect immunofluorescence and enzyme immunohistology procedures; flow cytometry, including DNA ploidy/cell cycle analysis; anti-neutrophil cytoplasmic antibodies(ANCA) and anti-nuclear antibodies; enzyme immunoassay; in situ hybridization; and direct and indirect in situ PCR.  1, record 12, English, - indirect%20PCR%20in%20situ

Record 12,  Textual support,  French

Record number: 12, Textual support number: 1 CONT

Son principe est basé sur l'incorporation des nucléotides non marqués. La détection des produits amplifiés est réalisée par hybridation in situ classique grâce à des sondes radiomarquées ou couplées à un haptène.  1, record 12, French, - PCR%20in%20situ%20indirecte

Record number: 12, Textual support number: 1 OBS

Cette méthode de révélation est très spécifique et peut être comparée dans son principe au transfert de Southern (Southern blot) qui est, entre autres, employé pour confirmer les résultats obtenus par PCR liquide.  1, record 12, French, - PCR%20in%20situ%20indirecte

Record 12,  Textual support,  Spanish

Record 13,  Textual support,  English

Record number: 13, Textual support number: 1 CONT

In a chromosomal map, genes or other identifiable DNA fragments are assigned to their respective chromosomes, with distances measured in base pairs. These markers can be physically associated with particular bands(identified by cytogenetic staining) primarily by in situ hybridization, a technique that involves tagging the DNA marker with an observable label(e. g.) one that fluorescesces or is radioactive). The location of the labeled probe can be detected after its binds to its complementary DNA strand in an intact chromosome.  1, record 13, English, - chromosomal%20map

Record 13,  Textual support,  French

Record number: 13, Textual support number: 1 DEF

Représentation de points de repères cytologiques d'un chromosome, reliés à des loci connus par des mutations et leur position sur une carte génétique.  1, record 13, French, - carte%20chromosomique

Record number: 13, Textual support number: 1 OBS

Définition tirée d'un vocabulaire dans INTERNET.  2, record 13, French, - carte%20chromosomique

Record 13,  Textual support,  Spanish

Record 14,  Textual support,  English

Record number: 14, Textual support number: 1 CONT

Two strategies have been used to clone specific sequences of eukaryotic, genomic DNA in bacteriophage lambda vectors. The first involved cloning of DNA preparations that had been highly enriched for the sequences of interest. Total genomic DNA was digested to completion with a restriction endonuclease and the resulting fragments were separated by preparative gel electrophoresis, reverse-phase column chromatography, or both. The fractions containing the DNA sequences of interest were identified by hybridization to appropriate probes and cloned in bacteriophage lambda vectors. Because these fractionation procedures may yield an overall enrichment of as much as 100-fold to 300-fold, the number of recombinant bacteriophages that had to be generated and screened for the DNA sequences of interest could be significantly reduced. Subsequently, the development of efficient in vitro packaging systems and in situ hybridization techniques eliminated the need to enrich the starting DNA for the sequences of interest.  1, record 14, English, - reverse%20phase%20column%20chromatography

Record 14,  Textual support,  French

Record 14,  Textual support,  Spanish

Record 15,  Textual support,  English

Record number: 15, Textual support number: 1 CONT

The random primer labeling technique applied to in situ hybridization on tissue sections.  1, record 15, English, - random%20primer

Record number: 15, Textual support number: 1 OBS

Term and example extracted from the PASCAL data base.  1, record 15, English, - random%20primer

Record 15,  Textual support,  French

Record 15,  Textual support,  Spanish