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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

In order to analyze the transgene integrated, the isolated gene samples, which are detected with transgene integration, undergo restriction digestion with known restriction endonucleases.  2, record 1, English, - transgene%20integration

Record 1,  Textual support,  French

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

Treated with these two genomes, the packaging cell produces a crop of retroviruses with: the envelope protein needed to infect the human target cells; an RNA copy of the human ADA [adenosine deaminase] gene, complete with R sequences at each end; reverse transcriptase, needed to make a DNA copy of the ADA gene that can be inserted into the DNA of the target cell and none of the genes (gag, pol, env) that would enable the virus to replicate in its new host.  3, record 2, English, - packaging%20cell

Record number: 2, Textual support number: 2 CONT

To create the retroviral vectors that are used in gene therapy, the life-cycles of their naturally occurring counterparts are exploited. The transgene(in this case, the gene for factor IX) in a vector backbone is put into a packaging cell, which expresses the genes that are required for viral integration.  4, record 2, English, - packaging%20cell

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

Les différents modes de transfert sont : [...] Injection de cellules produisant le vecteur viral pour éviter son inactivation trop rapide par le complément. Ces cellules sont appelés cellules transcomplémentantes (ou cellules d'encapsidation).  3, record 2, French, - cellule%20d%27encapsidation

Record number: 2, Textual support number: 1 OBS

Il s'agit d'un type de cellules utilisées pour produire des virus non pathogènes en supprimant les gènes nécessaires à leur réplication et en les remplaçant par une séquence choisie et des éléments régulateurs.  4, record 2, French, - cellule%20d%27encapsidation

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 CONT

The utility of tyrosinase minigene co-injection was evaluated as a visual marker for the generation and breeding of transgenic mice. In an evaluation of 39 transgenic founder animals and 44 transgenic lines five phenotypic patterns of pigmentation were consistently observed, including albino, dark, light, mottled and himalayan. In these studies co-injection of the tyrosinase minigene along with the transgene of interest(TOI) resulted in genomic integration of the two transgenes in 95% of the F0 generation.  2, record 3, English, - minigene

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 DEF

Gène reconstruit à des fins expérimentales à partir de ses séquences régulatrices et de l'ADNc double brin de l'ARNm correspondant.  1, record 3, French, - minig%C3%A8ne

Record number: 3, Textual support number: 1 OBS

Un minigène code donc directement pour la forme mature de l'ARNm.  1, record 3, French, - minig%C3%A8ne

Record 3,  Textual support,  Spanish