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UNIVERSAL SEQUENCING PRIMER LINKER [1 record]

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Record 1 1987-05-20

English

Subject field(s)
  • Genetics
DEF

A new method for direct sequence analysis of DNA restriction fragments uses synthetic covalently linked complementary oligodeoxynucleotides, as universal sequencing primer linkers (splinkers). These splinkers were designed to contain an inverted repeat sequence which forms a double-stranded hairpin structure with a known restriction site. The splinkers were characterized by their ability to be self-ligating (dimerized) and by their restriction digest product analysis of both the monomer and dimer.

OBS

Taken from ’Medline’ data base.

French

Domaine(s)
  • Génétique

Spanish

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