TERMIUM Plus®
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AFFINITY PURIFICATION [2 records]
Record 1 - internal organization data 2011-05-17
Record 1, English
Record 1, Subject field(s)
- Analytical Chemistry
- Biochemistry
- Biotechnology
Record 1, Main entry term, English
- affinity chromatography
1, record 1, English, affinity%20chromatography
correct
Record 1, Abbreviations, English
- AC 2, record 1, English, AC
correct
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
A procedure which uses a gel matrix combined with an enzyme or nucleic acid to obtain purification of biomolecules. 3, record 1, English, - affinity%20chromatography
Record number: 1, Textual support number: 2 DEF
A technique of molecular separation in which molecules are attached to an insoluble (e.g., sepharose) matrix. Only those molecules which show affinity to the bound molecule (e.g., an antibody for its antigen) are retained. These trapped molecules can be subsequently eluted. 4, record 1, English, - affinity%20chromatography
Record 1, Key term(s)
- affinity purification
Record 1, French
Record 1, Domaine(s)
- Chimie analytique
- Biochimie
- Biotechnologie
Record 1, Main entry term, French
- chromatographie d'affinité
1, record 1, French, chromatographie%20d%27affinit%C3%A9
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- chromatographie par affinité 2, record 1, French, chromatographie%20par%20affinit%C3%A9
feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Technique permettant la séparation de substances, [...] qui utilise la fixation spécifique d'une protéine sur un ligand lui-même fixé par liaison covalente à un support microscopique. 3, record 1, French, - chromatographie%20d%27affinit%C3%A9
Record number: 1, Textual support number: 1 CONT
[...] la chromatographie d'affinité permet de purifier une protéine enzymatique par exemple. La méthode consiste à attacher de façon covalente sur un support solide un analogue stérique du substrat de l'enzyme (inhibiteur compétitif ou substrat modifié) en vue de former avec l'enzyme un complexe du type enzyme-substrat [...]. L'enzyme sera donc retardé lors du passage sur la colonne par rapport aux autres protéines qui, ne reconnaissant pas l'analogue, sortiront de la colonne plus rapidement que l'enzyme. 4, record 1, French, - chromatographie%20d%27affinit%C3%A9
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Química analítica
- Bioquímica
- Biotecnología
Record 1, Main entry term, Spanish
- cromatografía por afinidad
1, record 1, Spanish, cromatograf%C3%ADa%20por%20afinidad
correct, feminine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
- cromatografía de afinidad 2, record 1, Spanish, cromatograf%C3%ADa%20de%20afinidad
feminine noun
Record 1, Textual support, Spanish
Record 2 - internal organization data 2010-05-19
Record 2, English
Record 2, Subject field(s)
- Biological Sciences
Record 2, Main entry term, English
- affinity purification
1, record 2, English, affinity%20purification
correct
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, Textual support, English
Record number: 2, Textual support number: 1 DEF
Purification technique based on a selective and reversible formation of a complex between the substance to be purified and another substance, most often called the ligand. 2, record 2, English, - affinity%20purification
Record number: 2, Textual support number: 1 CONT
Although affinity chromatography is still the dominant affinity purification technique, others - such as batch affinity adsorption, affinity precipitation and affinity partitioning - offer certain advantages in large-scale work.... Enzymes normally display highly specific interactions with their substrates. Advantage can be taken of this specificity in the so-called affinity separation methods of enzyme purification, i.e. affinity chromatography and affinity elution. 3, record 2, English, - affinity%20purification
Record number: 2, Textual support number: 1 OBS
Depending on the way in which the ligand is derivatized and physically contained, affinity purification methods are classified as belonging to one of the following three categories: affinity adsorption, affinity precipitation and affinity partitioning. 3, record 2, English, - affinity%20purification
Record 2, French
Record 2, Domaine(s)
- Sciences biologiques
Record 2, Main entry term, French
- purification par affinité
1, record 2, French, purification%20par%20affinit%C3%A9
correct, feminine noun
Record 2, Abbreviations, French
Record 2, Synonyms, French
Record 2, Textual support, French
Record number: 2, Textual support number: 1 CONT
De nombreux travaux tentent d'améliorer la productivité de la purification par affinité en l'adaptant (...) à des procédés de séparation capables de traiter des grands volumes en un minimum de temps. Il s'agit de la filtration par affinité à contre-courant, de la séparation d'affinité par partage de phase et de la précipitation par affinité. 1, record 2, French, - purification%20par%20affinit%C3%A9
Record 2, Spanish
Record 2, Textual support, Spanish
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