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AGAROSE GEL ELECTROPHORESIS [1 record]
Record 1 - internal organization data 2010-04-19
Record 1, English
Record 1, Subject field(s)
- Genetics
- Biotechnology
Record 1, Main entry term, English
- agarose gel electrophoresis
1, record 1, English, agarose%20gel%20electrophoresis
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
A method of separating molecules of different molecular weight or of the same molecular weight but different configuration by passing them through a gel column under the influence of an applied electric field. 2, record 1, English, - agarose%20gel%20electrophoresis
Record number: 1, Textual support number: 1 OBS
In general, higher molecular weight molecules pass through faster than lower molecular weight molecules, the migration rates of the molecules being inversely proportional to the logarithms of their molecular weights. Represented a breakthrough in rec DNA technology since it was the first accurate method of sizing DNA molecules. 2, record 1, English, - agarose%20gel%20electrophoresis
Record 1, French
Record 1, Domaine(s)
- Génétique
- Biotechnologie
Record 1, Main entry term, French
- électrophorèse en gel d'agarose
1, record 1, French, %C3%A9lectrophor%C3%A8se%20en%20gel%20d%27agarose
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- électrophorèse sur gel d'agarose 2, record 1, French, %C3%A9lectrophor%C3%A8se%20sur%20gel%20d%27agarose
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Méthode de fractionnement, qui opère une ségrégation des molécules d'ADN en fonction de la taille. Le support est constitué à l'aide d'une solution de gélose (agar-agar) à 1 p. 100 dans le tampon choisi. On la coule à chaud sur une lame de verre où elle se solidifie par refroidissement. Le pouvoir de résolution est identique à celui du papier. Mais le gel d'agarose purifiée présente l'avantage de ne pas produire d'interaction avec les protéines. 3, record 1, French, - %C3%A9lectrophor%C3%A8se%20en%20gel%20d%27agarose
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Genética
- Biotecnología
Record 1, Main entry term, Spanish
- electroforesis en gel de agarosa
1, record 1, Spanish, electroforesis%20en%20gel%20de%20agarosa
correct, feminine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 DEF
[Método] para analizar y caracterizar ácidos nucleicos de distintas procedencias. 1, record 1, Spanish, - electroforesis%20en%20gel%20de%20agarosa
Record number: 1, Textual support number: 1 CONT
Los geles se comportan como un tamiz molecular y permiten separar moléculas cargadas en función de su tamaño y forma. Así, moléculas de DNA de diferente tamaño van a emigrar de forma distinta en una electroforesis en gel de agarosa. Además, si en dicha electroforesis se aplican marcadores de peso molecular (fragmentos de DNA de tamaño conocido) se puede calcular el tamaño aproximado del DNA en estudio. 1, record 1, Spanish, - electroforesis%20en%20gel%20de%20agarosa
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