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ARTIFICIAL GENE [1 record]

Record 1 2012-07-11

English

Subject field(s)
  • Genetics
  • Biotechnology
DEF

A double-stranded DNA molecule carrying a specific sequence that will code for a given amino acid sequence and that has been produced in vitro.

OBS

It may be synthesized using an enzyme technique to form a strand of DNA on an mRNA template using reverse transcriptase and DNA polymerase to convert the single-stranded product (cDNA) to double-stranded DNA. Alternatively, an entirely new gene may be created, which will code for a novel amino acid sequence, by using a template formed from chemically synthesized oligonucleotides followed by enzymic polymerization.

French

Domaine(s)
  • Génétique
  • Biotechnologie
DEF

Gène créé par assemblage d'oligonucléotides, résultant d'une synthèse chimique, in vitro. [Définition normalisée par l'AFNOR et reproduite avec son autorisation.]

OBS

gène artificiel; gène de synthèse; gène synthétique : termes normalisés par l'AFNOR et publiés au Journal officiel de la République française le 22 septembre 2000.

Spanish

Campo(s) temático(s)
  • Genética
  • Biotecnología
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