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SELECTIVE SYSTEM [1 record]
Record 1 - internal organization data 2013-11-15
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- selective system
1, record 1, English, selective%20system
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
An experimental technique that enhances the recovery of specific (usually rare) genotypes. Littlefield (1964) isolated the rare products of spontaneous fusion in mixed cultures by a technique adopted from microbial genetics. Fusion of two cells deficient in two different enzymes resulted in hybrids that recovered the complete enzyme set by complementation. Only these cells survived selection against the deficient cells. 2, record 1, English, - selective%20system
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- système sélectif
1, record 1, French, syst%C3%A8me%20s%C3%A9lectif
correct, masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Système pour l'isolement sélectif de cellules rares. Un de ces systèmes, inventé en 1964 par Littlefield, est couramment employé. Il est fondé sur l'utilisation, en vue de fusion et d'hybridation, de mutants cellulaires présentant des déficiences enzymatiques (telles que l'absence de pyrophosphorylase d'acide inosinique ou de thymidinekinase) qui rendent ces mutants incapables de se multiplier dans un milieu sélectif spécialement conçu et contenant, en plus de précurseurs des acides nucléiques, de l'aminoptérine. Dans ces conditions, seules les cellules hybrides dans lesquelles se reconstitue, par une compensation génétique réciproque, l'appareil enzymatique intégral sont capables d'accomplir le cycle normal de synthèse d'acides nucléiques et, par conséquent, de se reproduire. 2, record 1, French, - syst%C3%A8me%20s%C3%A9lectif
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Genética
Record 1, Main entry term, Spanish
- sistema selectivo
1, record 1, Spanish, sistema%20selectivo
correct, masculine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
Record 1, Textual support, Spanish
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