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WIDE FIELD DECONVOLUTION MICROSCOPY [1 record]
Record 1 - internal organization data 2017-01-10
Record 1, English
Record 1, Subject field(s)
- Bioengineering
- Molecular Biology
Record 1, Main entry term, English
- wide field deconvolution microscopy
1, record 1, English, wide%20field%20deconvolution%20microscopy
proposal
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 OBS
deconvolution microscope : using the deconvolution microscope, researchers can see the locations of target molecules inside the cell. Seeing where glowing molecules reside within a cell is helpful for a range of research projects -- from studies of how the distribution of various proteins changes during an organism's development to investigations of how administration of a drug alters the locations of certain proteins. The microscope, called DeltaVision, is manufactured by Applied Precision, based in Issaquah, Wash. It works by magnifying images and quantifying the intensity of light shed by the sample. The user specifies which colors of light are of interest. For instance, a user can program the microscope to report the intensity of a particular shade of green shining from the cytoplasm of a cell. After a researcher uses the microscope to make a series of optical slices through a specimen, the scope's computer "deconvolves" the images -- in other words, it subtracts the out-of-focus light.Then the computer stacks together the images of the slices to reconstruct the original microscope sample. This gives researchers gh-resolution images of the sample's three-dimensional structure. 2, record 1, English, - wide%20field%20deconvolution%20microscopy
Record 1, French
Record 1, Domaine(s)
- Technique biologique
- Biologie moléculaire
Record 1, Main entry term, French
- microscopie de déconvolution à champ large
1, record 1, French, microscopie%20de%20d%C3%A9convolution%20%C3%A0%20champ%20large
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La microscopie de déconvolution à champ large est réalisée avec un microscope à fluorescence de facture classique. Les images obtenues sont donc «parasitées» par la fluorescence émise hors du plan de focalisation. Toutefois, un traitement informatique permet d'éliminer ce problème [...] Cette technique [...] permet d'utiliser des intensités d'illumination beaucoup plus faibles que celles qui sont mises en œuvre par la microscopie confocale. 1, record 1, French, - microscopie%20de%20d%C3%A9convolution%20%C3%A0%20champ%20large
Record number: 1, Textual support number: 1 OBS
Tiré du Technoscope. 2, record 1, French, - microscopie%20de%20d%C3%A9convolution%20%C3%A0%20champ%20large
Record 1, Spanish
Record 1, Textual support, Spanish
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