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5B7 CAPTURE MAB [1 record]

Record 1 2002-04-22

English

Subject field(s)
  • Immunology
  • Molecular Biology
  • Animal Diseases
OBS

In the Elisa [enzyme-linked immunosorbent assay] developed by Brocchi ... the SVD [swine vesicular disease] viral antigen is trapped to the solid phase using the MAB 5B7. The ability of test sera to inhibit the binding of peroxidase-conjugated MAB 5B7 to the trapped antigen is then evaluated.

OBS

A sandwich Elisa [enzyme-linked immunosorbent assay] can also be performed: the sample is first allowed to react with the detection MAB and the immunocomplex is then captured by the second MAB adsorbed on to the Elisa plate.

OBS

5B7 is a type of monoclonal antibody.

French

Domaine(s)
  • Immunologie
  • Biologie moléculaire
  • Maladies des animaux
OBS

Pour détecter les antigènes de la maladie vésiculeuse du porc et effectuer un diagnostic différentiel par rapport à la fièvre aphteuse dans des échantillons d'éphithélium, de fluide vésiculaire ou de cultures de tissus infectés, il est également possible d'utiliser une technique Elisa [dosage immunoenzymatique] aux anticorps monoclonaux faisant appel à des anticorps monoclonaux sélectionnés comme pièges et à des anticorps monoclonaux conjugués à une peroxydase comme détecteurs.

OBS

Cette technique Elisa [dosage immunoenzymatique] concurrentielle utilise un anticorps monoclonal comme anticorps de capture et un anti-antisérum de souris d'affinité conjugué à la peroxydase comme anticorps de détection.

OBS

5B7 est un type d'anticorps monoclonal.

Spanish

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