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DNA SEQUENCING [6 records]
Record 1 - internal organization data 2011-08-25
Record 1, English
Record 1, Subject field(s)
- Scientific Research Equipment
- Genetics
Record 1, Main entry term, English
- Macrophor DNA sequencing system
1, record 1, English, Macrophor%20DNA%20sequencing%20system
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, French
Record 1, Domaine(s)
- Matériel et équipement (Recherche scientifique)
- Génétique
Record 1, Main entry term, French
- système de séquençage d'ADN Macrophor
1, record 1, French, syst%C3%A8me%20de%20s%C3%A9quen%C3%A7age%20d%27ADN%20Macrophor
masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record 1, Spanish
Record 1, Textual support, Spanish
Record 2 - internal organization data 2011-07-28
Record 2, English
Record 2, Subject field(s)
- Genetics
Record 2, Main entry term, English
- DNA sequencing system
1, record 2, English, DNA%20sequencing%20system
correct
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, French
Record 2, Domaine(s)
- Génétique
Record 2, Main entry term, French
- système de séquençage d'ADN
1, record 2, French, syst%C3%A8me%20de%20s%C3%A9quen%C3%A7age%20d%27ADN
masculine noun
Record 2, Abbreviations, French
Record 2, Synonyms, French
Record 2, Textual support, French
Record 2, Spanish
Record 2, Textual support, Spanish
Record 3 - internal organization data 2009-10-26
Record 3, English
Record 3, Subject field(s)
- Biochemistry
- Genetics
- Diagnostic Procedures (Medicine)
Record 3, Main entry term, English
- DNA sequencing
1, record 3, English, DNA%20sequencing
correct
Record 3, Abbreviations, English
Record 3, Synonyms, English
Record 3, Textual support, English
Record number: 3, Textual support number: 1 DEF
The determination of the sequence of nucleotides making up a length of DNA. 2, record 3, English, - DNA%20sequencing
Record number: 3, Textual support number: 1 CONT
Confirmation of positive results can be done with a NAAT [nucleic acid amplification test] using a different set of primers or by DNA sequencing techniques. 3, record 3, English, - DNA%20sequencing
Record 3, Key term(s)
- DNA-sequencing
Record 3, French
Record 3, Domaine(s)
- Biochimie
- Génétique
- Méthodes diagnostiques (Médecine)
Record 3, Main entry term, French
- séquençage de l'ADN
1, record 3, French, s%C3%A9quen%C3%A7age%20de%20l%27ADN
correct, masculine noun
Record 3, Abbreviations, French
Record 3, Synonyms, French
Record 3, Textual support, French
Record number: 3, Textual support number: 1 DEF
Établissement de la séquence des nucléotides d'un brin d'ADN. 2, record 3, French, - s%C3%A9quen%C3%A7age%20de%20l%27ADN
Record 3, Spanish
Record 3, Campo(s) temático(s)
- Bioquímica
- Genética
- Procedimientos diagnósticos (Medicina)
Record 3, Main entry term, Spanish
- secuenciación de ADN
1, record 3, Spanish, secuenciaci%C3%B3n%20de%20ADN
feminine noun
Record 3, Abbreviations, Spanish
Record 3, Synonyms, Spanish
Record 3, Textual support, Spanish
Record 4 - internal organization data 1999-05-13
Record 4, English
Record 4, Subject field(s)
- Titles of Monographs
- Sciences - General
- Commercial Fishing
Record 4, Main entry term, English
- Amplification and sequencing of control region mitochondrial DNA from the Beluga whale
1, record 4, English, Amplification%20and%20sequencing%20of%20control%20region%20mitochondrial%20DNA%20from%20the%20Beluga%20whale
correct, Canada
Record 4, Abbreviations, English
Record 4, Synonyms, English
Record 4, Textual support, English
Record number: 4, Textual support number: 1 OBS
Fisheries and Oceans Canada. Serie: Canadian Technical Report of Fisheries and Aquatic Sciences. 1, record 4, English, - Amplification%20and%20sequencing%20of%20control%20region%20mitochondrial%20DNA%20from%20the%20Beluga%20whale
Record 4, French
Record 4, Domaine(s)
- Titres de monographies
- Sciences - Généralités
- Pêche commerciale
Record 4, Main entry term, French
- Amplification and sequencing of control region mitochondrial DNA from the Beluga whale
1, record 4, French, Amplification%20and%20sequencing%20of%20control%20region%20mitochondrial%20DNA%20from%20the%20Beluga%20whale
correct, Canada
Record 4, Abbreviations, French
Record 4, Synonyms, French
Record 4, Textual support, French
Record 4, Spanish
Record 4, Textual support, Spanish
Record 5 - internal organization data 1991-07-16
Record 5, English
Record 5, Subject field(s)
- Genetics
Record 5, Main entry term, English
- multiplex DNA sequencing
1, record 5, English, multiplex%20DNA%20sequencing
correct
Record 5, Abbreviations, English
Record 5, Synonyms, English
Record 5, Textual support, English
Record number: 5, Textual support number: 1 CONT
Multiplex DNA sequencing: The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N. Such a method is described. In order to separate the sequence information at the end of the processing, the DNA molecules of interest are ligated to a set of oligonucleotide "tags" at the beginning. The tagged DNA molecules are pooled, amplified, and chemically fragmented in 96-well plates. The resulting reaction products are fractionated by size on sequencing gels and transferred to nylon membranes. These membranes are then probed as many times as there are types of tags in the original pools, producing, in each cycle of probing, autoradiographs similar to those from standard DNA sequencing methods. 1, record 5, English, - multiplex%20DNA%20sequencing
Record 5, French
Record 5, Domaine(s)
- Génétique
Record 5, Main entry term, French
- méthode de séquençage multiplex 1, record 5, French, m%C3%A9thode%20de%20s%C3%A9quen%C3%A7age%20multiplex
Record 5, Abbreviations, French
Record 5, Synonyms, French
Record 5, Textual support, French
Record number: 5, Textual support number: 1 CONT
La méthode de séquençage multiplex : Plusieurs bibliothèques génomiques sont construites dans des vecteurs plasmidiques contenant chacun une séquence nucléotidique particulière, séquence cible spécifique. Des lots de 20 colonies provenant de chacune des 20 bibliothèques sont alors formés et les plasmides de chaque lot sont purifiés. La digestion enzymatique permet d'exciser les inserts contenant les fragments d'ADN génomique flanqués de leurs deux séquences cibles. Les opérations de séquençage sont alors effectuées selon la méthode chimique de Maxam et Gilbert, et les fragments obtenus sont séparés sur gel de polyacrylamide par électrophorèse. Une sonde correspondant à la séquence caractéristique du vecteur est utilisée pour révéler les fragments issus de ce clone. 1, record 5, French, - m%C3%A9thode%20de%20s%C3%A9quen%C3%A7age%20multiplex
Record 5, Spanish
Record 5, Textual support, Spanish
Record 6 - internal organization data 1987-05-22
Record 6, English
Record 6, Subject field(s)
- Scientific Research Equipment
- Medical Instruments and Devices
- Biochemistry
Record 6, Main entry term, English
- DNA/RNA sequencing system
1, record 6, English, DNA%2FRNA%20sequencing%20system
correct
Record 6, Abbreviations, English
Record 6, Synonyms, English
Record 6, Textual support, English
Record number: 6, Textual support number: 1 CONT
LKB Macrophor DNA/RNA Sequencing System: For sequencing of DNA and RNA. New separation techniques involve the generation of a set of oligonucleotides having one end in common and differing in length in steps of one nucleotide, followed by electrophoretic separation of the oligonucleotides in a denaturing polyacrylamide gel. 1, record 6, English, - DNA%2FRNA%20sequencing%20system
Record 6, French
Record 6, Domaine(s)
- Matériel et équipement (Recherche scientifique)
- Instruments et appareillages médicaux
- Biochimie
Record 6, Main entry term, French
- système séquenceur d'ADN/ARN
1, record 6, French, syst%C3%A8me%20s%C3%A9quenceur%20d%27ADN%2FARN
correct, masculine noun
Record 6, Abbreviations, French
Record 6, Synonyms, French
Record 6, Textual support, French
Record number: 6, Textual support number: 1 CONT
Système séquenceur d'ADN/ARN Macrophor LKB : Des nouvelles techniques de séparation impliquent la génération d'un ensemble d'oligonucléotides ayant une extrémité en commun et différant en longueur en phases d'un nucléotide, suivi d'une séparation électrophorétique d'oligonucléotides dans un gel de polyacrylamide dénaturé. 1, record 6, French, - syst%C3%A8me%20s%C3%A9quenceur%20d%27ADN%2FARN
Record 6, Spanish
Record 6, Textual support, Spanish
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