TERMIUM Plus®
The Government of Canada’s terminology and linguistic data bank.
POLYMERASE CHAIN REACTION [11 records]
Record 1 - internal organization data 2023-03-30
Record 1, English
Record 1, Subject field(s)
- Biochemistry
- Genetics
- Diagnostic Procedures (Medicine)
Record 1, Main entry term, English
- quantitative PCR
1, record 1, English, quantitative%20PCR
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- quantitative polymerase chain reaction 2, record 1, English, quantitative%20polymerase%20chain%20reaction
correct
- QPCR 3, record 1, English, QPCR
avoid, see observation
- QPCR 3, record 1, English, QPCR
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Quantitative PCR ... indicates not just whether a specific DNA segment is present in a sample, but also how much of it is there. ... Quantitative PCR is used ... to help search for and evaluate targets, i.e. the sites in the body at which new drugs can act. ... the number of working copies of a specific gene [can be] measured by quantitative PCR. ... By comparing the profiles in healthy and ill people it is possible to determine ... whether a gene ... is associated with the development or progression of a disease. ... Another important application of quantitative PCR is in molecular diagnosis, i.e. the diagnosis of diseases [particularly viral diseases] based on molecular findings rather than on physiological symptoms. 4, record 1, English, - quantitative%20PCR
Record number: 1, Textual support number: 1 OBS
PCR consists in the selective amplification of a given DNA sequence from a pathogen's genome. ... PCR can be quantitative, i.e. it can evaluate the viral or bacterial load (number of pathogen per milliliter of blood), which can be very useful to follow the effect of an antibiotic or antiviral therapy. 5, record 1, English, - quantitative%20PCR
Record number: 1, Textual support number: 2 OBS
QPCR: Although this abbreviation is widely used to refer to "quantitative PCR", it is also sometimes used to refer to "qualitative PCR". 6, record 1, English, - quantitative%20PCR
Record 1, French
Record 1, Domaine(s)
- Biochimie
- Génétique
- Méthodes diagnostiques (Médecine)
Record 1, Main entry term, French
- PCR quantitative
1, record 1, French, PCR%20quantitative
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- réaction de polymérisation en chaîne quantitative 2, record 1, French, r%C3%A9action%20de%20polym%C3%A9risation%20en%20cha%C3%AEne%20quantitative
correct, feminine noun, rare
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
[...] le virus se multiplie activement dans le foie et peut être détecté dans le sang par [la] PCR qui permet d'amplifier la présence de brins de virus actif (ARN). Il existe deux modalités, l'une globale ou PCR qualitative, l'autre plus précise mesurant le nombre de particules virales circulantes ou PCR quantitative [qui permet] de mesurer la charge virale avant [un] traitement et d'en suivre le taux durant celui-ci. 3, record 1, French, - PCR%20quantitative
Record number: 1, Textual support number: 2 CONT
La PCR quantitative [...] ne détecte pas des quantités de virus aussi faibles que la PCR qualitative, mais elle permet de mesurer la quantité de virus présent dans l'organisme. C'est un reflet de l'importance de la multiplication du virus [...] Elle est également utile lors de la surveillance du traitement. Elle permet d'évaluer son efficacité et [de] savoir s'il faut le poursuivre ou non [...] 4, record 1, French, - PCR%20quantitative
Record 1, Spanish
Record 1, Textual support, Spanish
Record 2 - external organization data 2022-05-10
Record 2, English
Record 2, Subject field(s)
- Compartment - Royal Canadian Mounted Police (RCMP)
Universal entry(ies) Record 2
Record 2, Main entry term, English
- Polymerase Chain Reaction Analyst 1, record 2, English, Polymerase%20Chain%20Reaction%20Analyst
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, Textual support, English
Record number: 2, Textual support number: 1 OBS
3556: Royal Canadian Mounted Police job code. 1, record 2, English, - Polymerase%20Chain%20Reaction%20Analyst
Record 2, Key term(s)
- PCR Analyst
Record 2, French
Record 2, Domaine(s)
- Tiroir - Gendarmerie royale du Canada (GRC)
Entrée(s) universelle(s) Record 2
Record 2, Main entry term, French
- analyste de l'amplification en chaîne par polymérase
1, record 2, French, analyste%20de%20l%27amplification%20en%20cha%C3%AEne%20par%20polym%C3%A9rase
masculine and feminine noun
Record 2, Abbreviations, French
Record 2, Synonyms, French
Record 2, Textual support, French
Record number: 2, Textual support number: 1 OBS
3556 : code d'emploi de la Gendarmerie royale du Canada. 1, record 2, French, - analyste%20de%20l%27amplification%20en%20cha%C3%AEne%20par%20polym%C3%A9rase
Record 2, Key term(s)
- analyste de l'ACP
Record 2, Spanish
Record 2, Textual support, Spanish
Record 3 - internal organization data 2021-05-19
Record 3, English
Record 3, Subject field(s)
- Biotechnology
- Genetics
- Molecular Biology
Record 3, Main entry term, English
- polymerase chain reaction
1, record 3, English, polymerase%20chain%20reaction
correct
Record 3, Abbreviations, English
- PCR 2, record 3, English, PCR
correct
Record 3, Synonyms, English
Record 3, Textual support, English
Record number: 3, Textual support number: 1 CONT
The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. 3, record 3, English, - polymerase%20chain%20reaction
Record number: 3, Textual support number: 1 OBS
A technique used to make numerous copies of a DNA segment. 4, record 3, English, - polymerase%20chain%20reaction
Record number: 3, Textual support number: 2 OBS
The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. 3, record 3, English, - polymerase%20chain%20reaction
Record number: 3, Textual support number: 1 PHR
PCR test 4, record 3, English, - polymerase%20chain%20reaction
Record 3, French
Record 3, Domaine(s)
- Biotechnologie
- Génétique
- Biologie moléculaire
Record 3, Main entry term, French
- amplification en chaîne par polymérase
1, record 3, French, amplification%20en%20cha%C3%AEne%20par%20polym%C3%A9rase
correct, feminine noun
Record 3, Abbreviations, French
- PCR 2, record 3, French, PCR
correct, feminine noun
- ACP 3, record 3, French, ACP
correct, feminine noun, rare
Record 3, Synonyms, French
- réaction en chaîne de la polymérase 4, record 3, French, r%C3%A9action%20en%20cha%C3%AEne%20de%20la%20polym%C3%A9rase
correct, feminine noun
- PCR 2, record 3, French, PCR
correct, feminine noun
- RCP 5, record 3, French, RCP
correct, feminine noun
- PCR 2, record 3, French, PCR
- réaction de polymérisation en chaîne 6, record 3, French, r%C3%A9action%20de%20polym%C3%A9risation%20en%20cha%C3%AEne
correct, feminine noun
- PCR 2, record 3, French, PCR
correct, feminine noun
- PCR 2, record 3, French, PCR
Record 3, Textual support, French
Record number: 3, Textual support number: 1 OBS
Technique permettant d'obtenir un grand nombre de copies d'un fragment d'ADN. 6, record 3, French, - amplification%20en%20cha%C3%AEne%20par%20polym%C3%A9rase
Record number: 3, Textual support number: 2 OBS
Pour en isoler l'ADN, l'échantillon subit solubilisation, centrifugation et précipitation par de l'alcool. Le fragment d'ADN constitutif du transgène recherché est alors sélectivement amplifié, c'est-à-dire répliqué en de nombreux exemplaires. [...] on sépare les deux brins formant l'ADN par chauffage [...] puis on introduit dans le milieu de travail des amorces, qui sont les morceaux d'ADN encadrant le transgène. On ajoute aussi une enzyme, par exemple la Taq-polymérase, qui va, à partir des bases placées en excès dans le milieu, fabriquer le brin complémentaire d'ADN. [...] Pour séparer les transgènes des autres portions d'ADN, on pratique alors une électrophorèse, ou migration différentielle sur gel d'agarose. 7, record 3, French, - amplification%20en%20cha%C3%AEne%20par%20polym%C3%A9rase
Record number: 3, Textual support number: 3 OBS
PCR : L'abréviation «PCR» provient du terme anglais «polymerase chain reaction». 6, record 3, French, - amplification%20en%20cha%C3%AEne%20par%20polym%C3%A9rase
Record number: 3, Textual support number: 4 OBS
amplification en chaîne par polymérase; ACP : désignations publiées au Journal officiel de la République française le 23 novembre 2006. 8, record 3, French, - amplification%20en%20cha%C3%AEne%20par%20polym%C3%A9rase
Record 3, Spanish
Record 3, Campo(s) temático(s)
- Biotecnología
- Genética
- Biología molecular
Record 3, Main entry term, Spanish
- reacción en cadena de la polimerasa
1, record 3, Spanish, reacci%C3%B3n%20en%20cadena%20de%20la%20polimerasa
correct, feminine noun
Record 3, Abbreviations, Spanish
- RCP 2, record 3, Spanish, RCP
correct, feminine noun
Record 3, Synonyms, Spanish
Record 3, Textual support, Spanish
Record number: 3, Textual support number: 1 DEF
Técnica utilizada para hacer copias de moléculas específicas de ADN. 3, record 3, Spanish, - reacci%C3%B3n%20en%20cadena%20de%20la%20polimerasa
Record number: 3, Textual support number: 1 OBS
Se calienta la doble hélice de ADN al objeto de separar sus cadenas. La enzima (junto con las subunidades que conforman el ADN y unas breves secuencias cebadoras) fabrica nuevas copias de la hélice original. El proceso se repite ad infinitum. 3, record 3, Spanish, - reacci%C3%B3n%20en%20cadena%20de%20la%20polimerasa
Record 4 - internal organization data 2012-08-29
Record 4, English
Record 4, Subject field(s)
- Genetics
Record 4, Main entry term, English
- differential display
1, record 4, English, differential%20display
correct
Record 4, Abbreviations, English
- DD 2, record 4, English, DD
correct
Record 4, Synonyms, English
- DD reverse transcription polymerase chain reaction 3, record 4, English, DD%20reverse%20transcription%20polymerase%20chain%20reaction
correct
- DDRT-PCR 4, record 4, English, DDRT%2DPCR
correct
- DDRT-PCR 4, record 4, English, DDRT%2DPCR
Record 4, Textual support, English
Record number: 4, Textual support number: 1 CONT
Differential Display (DD) ... and a conceptually similar technique, arbitrarily primed PCR fingerprinting of RNA (APPCR)19, both PCR amplify subsets of an mRNA population and separate the resulting cDNAs by denaturing polyacrylamide gel electrophoresis. Since the introduction of DD in 1992, over 100 reports regarding improvements and/or successful applications have been published. Although DD has the appearance of being technically easy, the road from "band on the gel" to a positive can be treacherous as outlined in a recent commentary. The primary criticisms are (1) a high false positive rate, (2) questioned ability of DD to identify both abundant and rare mRNAs, (3) coding regions of mRNAs are usually not cloned, and (4) verification process is time consuming and usually requires a fair amount of RNA. 5, record 4, English, - differential%20display
Record 4, French
Record 4, Domaine(s)
- Génétique
Record 4, Main entry term, French
- technique du DD
1, record 4, French, technique%20du%20DD
proposal, feminine noun
Record 4, Abbreviations, French
Record 4, Synonyms, French
- differential display 1, record 4, French, differential%20display
anglicism
Record 4, Textual support, French
Record number: 4, Textual support number: 1 CONT
En 1992, deux chercheurs du Dana Farber Cancer Institute de Boston (États-Unis), P. Liang et AB Pardee, présentent une nouvelle méthode, le «differential display» (DD) - ou DDRT-PCR (DD reverse transcription polymerase chain reaction) qui permet une étude directe des modifications de l'expression génique [...] P. Liang et AB Pardee ont démontré que la combinaison d'amorces arbitraires et d'amorces spécifiques des ARNm permet de générer un set de fragments d'ADNc représentatifs des ARNm d'une cellule. Dans des conditions appropriées, le profil des fragments dérivés d'un type cellulaire est reproductible et peut être comparé à celui des fragments d'un autre type cellulaire. L'étude directe de l'expression différentielle des gènes devient possible. 2, record 4, French, - technique%20du%20DD
Record number: 4, Textual support number: 1 OBS
Outil d'expression génique. 1, record 4, French, - technique%20du%20DD
Record 4, Spanish
Record 4, Campo(s) temático(s)
- Genética
Record 4, Main entry term, Spanish
- visualización diferencial
1, record 4, Spanish, visualizaci%C3%B3n%20diferencial
correct, feminine noun
Record 4, Abbreviations, Spanish
Record 4, Synonyms, Spanish
Record 4, Textual support, Spanish
Record number: 4, Textual support number: 1 DEF
Método para identificar ARNm que se encuentra a diferentes concentraciones en diferentes tejidos o en respuesta a tratamientos específicos. 1, record 4, Spanish, - visualizaci%C3%B3n%20diferencial
Record number: 4, Textual support number: 1 OBS
Los ARNm se convierte a ADNc; una determinada proporción de estos ADNc se amplifican por la reacción en cadena de la polimerasa y se separan por electroforesis. 1, record 4, Spanish, - visualizaci%C3%B3n%20diferencial
Record 5 - internal organization data 2011-12-21
Record 5, English
Record 5, Subject field(s)
- Biotechnology
- Molecular Biology
Record 5, Main entry term, English
- polymerase chain reaction array
1, record 5, English, polymerase%20chain%20reaction%20array
correct
Record 5, Abbreviations, English
Record 5, Synonyms, English
- PCR-microarray 2, record 5, English, PCR%2Dmicroarray
correct
- PCR array 3, record 5, English, PCR%20array
correct
Record 5, Textual support, English
Record number: 5, Textual support number: 1 CONT
Each 96-well plate includes SYBR [Synergy Brands] Green-optimised primer sets for a thoroughly researched panel of relevant, pathway- or disease-focused genes. The arrays can also be customised to contain a panel of genes tailored to a specific research interests. The design and master mix formulation facilite the PCR [polymerase chain reaction] array to amplify 96 different gene-specific products simultaneously under uniform cycling conditions. 3, record 5, English, - polymerase%20chain%20reaction%20array
Record number: 5, Textual support number: 1 OBS
PCR: polymerase chain reaction. 4, record 5, English, - polymerase%20chain%20reaction%20array
Record 5, Key term(s)
- PCR micro-array
- PCR microarray
Record 5, French
Record 5, Domaine(s)
- Biotechnologie
- Biologie moléculaire
Record 5, Main entry term, French
- biopuce à produits PCR
1, record 5, French, biopuce%20%C3%A0%20produits%20PCR
correct, feminine noun
Record 5, Abbreviations, French
Record 5, Synonyms, French
- puce à produits PCR 2, record 5, French, puce%20%C3%A0%20produits%20PCR
correct, feminine noun
- biopuce pour la détection des produits PCR 3, record 5, French, biopuce%20pour%20la%20d%C3%A9tection%20des%20produits%20PCR
feminine noun
Record 5, Textual support, French
Record number: 5, Textual support number: 1 OBS
PCR : Réaction en chaîne de la polymérase. 3, record 5, French, - biopuce%20%C3%A0%20produits%20PCR
Record 5, Spanish
Record 5, Textual support, Spanish
Record 6 - internal organization data 2009-03-30
Record 6, English
Record 6, Subject field(s)
- Biochemistry
- Genetics
- Diagnostic Procedures (Medicine)
Record 6, Main entry term, English
- qualitative PCR
1, record 6, English, qualitative%20PCR
correct
Record 6, Abbreviations, English
Record 6, Synonyms, English
- qualitative polymerase chain reaction 2, record 6, English, qualitative%20polymerase%20chain%20reaction
correct, less frequent
Record 6, Textual support, English
Record number: 6, Textual support number: 1 CONT
PCR consists in the selective amplification of a given DNA sequence from a pathogen's genome. In qualitative PCR, the presence of an amplification product is checked by gel electrophoresis ... 3, record 6, English, - qualitative%20PCR
Record number: 6, Textual support number: 2 CONT
When PCR is used only for detecting a specific DNA segment, the method is referred to as qualitative PCR. ... Qualitative PCR is an extremely sensitive method which is theoretically able to detect a single DNA molecule in a sample solution. In many cases specific genes are copied in this way, e.g. in order to identify pathological changes. ... Qualitative PCR is also used around the world in forensic medicine to identify individuals. ... only qualitative PCR can determine whether an infection has been eradicated, whether it is chronic (and might therefore progress unnoticed) and whether the individual has been reinfected with a different but related pathogen. 4, record 6, English, - qualitative%20PCR
Record 6, French
Record 6, Domaine(s)
- Biochimie
- Génétique
- Méthodes diagnostiques (Médecine)
Record 6, Main entry term, French
- PCR qualitative
1, record 6, French, PCR%20qualitative
correct, feminine noun
Record 6, Abbreviations, French
Record 6, Synonyms, French
- réaction de polymérisation en chaîne qualitative 2, record 6, French, r%C3%A9action%20de%20polym%C3%A9risation%20en%20cha%C3%AEne%20qualitative
correct, feminine noun, rare
Record 6, Textual support, French
Record number: 6, Textual support number: 1 DEF
Examen de biologie moléculaire, réalisé dans des laboratoires spécialisés, permettant, grâce à une technique d'amplification, la détection des brins d'ARN dans le sérum. 3, record 6, French, - PCR%20qualitative
Record number: 6, Textual support number: 1 CONT
La PCR qualitative est une technique très sensible c'est-à-dire qu'elle détecte le virus même en très petite quantité dans le sang. [...] Elle est utile lors du diagnostic d'hépatite virale C. Par exemple, si on trouve des anticorps anti-VHC dans le sang d'un individu, [le] médecin fait [...] réaliser une PCR qualitative qui confirme ou non la persistance du virus dans l'organisme. [La PCR qualitative] est [aussi] utile lors de la surveillance d'un traitement. Par exemple, le médecin prescrit une PCR qualitative à la fin du traitement de l'hépatite puis 24 semaines après pour savoir si le virus est encore présent ou non, c'est-à-dire si le traitement a été efficace ou non. 4, record 6, French, - PCR%20qualitative
Record 6, Spanish
Record 6, Textual support, Spanish
Record 7 - internal organization data 2001-11-22
Record 7, English
Record 7, Subject field(s)
- Biotechnology
- Genetics
Record 7, Main entry term, English
- electronic polymerase chain reaction
1, record 7, English, electronic%20polymerase%20chain%20reaction
correct
Record 7, Abbreviations, English
- ePCR 2, record 7, English, ePCR
proposal
Record 7, Synonyms, English
Record 7, Textual support, English
Record number: 7, Textual support number: 1 DEF
Polymerase chain reaction technique that allows detection of sequence tagged sites (STSs) in DNA sequences to determine map locations for construction of various types of genomic maps. 1, record 7, English, - electronic%20polymerase%20chain%20reaction
Record 7, French
Record 7, Domaine(s)
- Biotechnologie
- Génétique
Record 7, Main entry term, French
- PCR électronique
1, record 7, French, PCR%20%C3%A9lectronique
correct, feminine noun
Record 7, Abbreviations, French
- PCRe 2, record 7, French, PCRe
correct, feminine noun
Record 7, Synonyms, French
- PCR in silico 1, record 7, French, PCR%20in%20silico
correct, feminine noun
Record 7, Textual support, French
Record number: 7, Textual support number: 1 CONT
...] méthode d'analyse de séquences : la PCR électronique (PCRe) ou PCR in silico. Cette méthode procure un gain de temps considérable car elle permet de détecter la présence de STS dans une séquence d'ADN sans avoir à faire d'expériences. Elle utilise une stratégie d'analyse informatique fondée sur le même principe que la PCR classique, d'où son nom. 1, record 7, French, - PCR%20%C3%A9lectronique
Record number: 7, Textual support number: 1 OBS
Tiré du Technoscope. 3, record 7, French, - PCR%20%C3%A9lectronique
Record 7, Spanish
Record 7, Textual support, Spanish
Record 8 - internal organization data 1997-07-04
Record 8, English
Record 8, Subject field(s)
- Plant and Crop Production
- Translation and Interpretation
Record 8, Main entry term, English
- duplex PCR
1, record 8, English, duplex%20PCR
correct
Record 8, Abbreviations, English
Record 8, Synonyms, English
- duplex polymerase chain reaction 2, record 8, English, duplex%20polymerase%20chain%20reaction
correct
Record 8, Textual support, English
Record number: 8, Textual support number: 1 CONT
A duplex PCR was developed to detect Potato Leaf Roll Virus and PVY from the same sample. PCR methods can detect nucleic acids. 1, record 8, English, - duplex%20PCR
Record 8, French
Record 8, Domaine(s)
- Cultures (Agriculture)
- Traduction et interprétation
Record 8, Main entry term, French
- réaction double en chaîne de la polymérase
1, record 8, French, r%C3%A9action%20double%20en%20cha%C3%AEne%20de%20la%20polym%C3%A9rase
proposal
Record 8, Abbreviations, French
Record 8, Synonyms, French
Record 8, Textual support, French
Record 8, Spanish
Record 8, Textual support, Spanish
Record 9 - internal organization data 1997-05-14
Record 9, English
Record 9, Subject field(s)
- Biochemistry
Record 9, Main entry term, English
- fluorescent PCR
1, record 9, English, fluorescent%20PCR
correct
Record 9, Abbreviations, English
Record 9, Synonyms, English
- fluorescent polymerase chain reaction 1, record 9, English, fluorescent%20polymerase%20chain%20reaction
correct
Record 9, Textual support, English
Record number: 9, Textual support number: 1 CONT
Fluorescent PCR: A New Technique for PGD of Sex and Single-Gene Defects. For single-cell diagnosis, particularly preimplantation genetic diagnosis to be successful four main criteria must be achieved: sensitivity, reliability, accuracy, and identification/elimination of contamination... Fluorescent PCR achieves all four necessary criteria and, in addition, currently allows genes on up to nine chromosomes to be simultaneously investigated. 1, record 9, English, - fluorescent%20PCR
Record 9, French
Record 9, Domaine(s)
- Biochimie
Record 9, Main entry term, French
- PCR fluorescente
1, record 9, French, PCR%20fluorescente
correct, feminine noun
Record 9, Abbreviations, French
Record 9, Synonyms, French
Record 9, Textual support, French
Record number: 9, Textual support number: 1 CONT
Le principe de la PCR fluorescente est d'utiliser des oligonucléotides marqués par un fluorochrome. La détection du signal fluorescent se fait à l'aide d'un séquenceur automatique. 1, record 9, French, - PCR%20fluorescente
Record number: 9, Textual support number: 1 OBS
Technique susceptible d'améliorer le diagnostic génétique préimplantatoire. 2, record 9, French, - PCR%20fluorescente
Record 9, Spanish
Record 9, Textual support, Spanish
Record 10 - internal organization data 1996-07-12
Record 10, English
Record 10, Subject field(s)
- Genetics
Record 10, Main entry term, English
- RCPCR
1, record 10, English, RCPCR
correct
Record 10, Abbreviations, English
Record 10, Synonyms, English
- recombinant circle polymerase chain reaction 2, record 10, English, recombinant%20circle%20polymerase%20chain%20reaction
proposal
- recombinant circle PCR 1, record 10, English, recombinant%20circle%20PCR
Record 10, Textual support, English
Record 10, French
Record 10, Domaine(s)
- Génétique
Record 10, Main entry term, French
- RCPCR
1, record 10, French, RCPCR
correct, feminine noun
Record 10, Abbreviations, French
Record 10, Synonyms, French
Record 10, Textual support, French
Record number: 10, Textual support number: 1 CONT
Principe de la RCPCR : produit d'une IPCR est mélangé avec celui d'une autre IPCR, laquelle est amorcée à une position différente, sur la même matrice d'origine. Après mélange, dénaturation et réhybridation, les plasmides recombinants hétéroduplex sont transformés. Par le même approche, la mutagénèse sur de très grands plasmides peut être envisagée. 1, record 10, French, - RCPCR
Record 10, Spanish
Record 10, Textual support, Spanish
Record 11 - internal organization data 1996-07-12
Record 11, English
Record 11, Subject field(s)
- Genetics
Record 11, Main entry term, English
- inverted polymerase chain reaction 1, record 11, English, inverted%20polymerase%20chain%20reaction
Record 11, Abbreviations, English
- IPCR 2, record 11, English, IPCR
correct
Record 11, Synonyms, English
- inverse PCR 3, record 11, English, inverse%20PCR
correct
- inverse-PCR 3, record 11, English, inverse%2DPCR
correct
- inside out PCR 4, record 11, English, inside%20out%20PCR
Record 11, Textual support, English
Record number: 11, Textual support number: 1 DEF
Technique that can be employed in the determination of an unknown sequence that flank a region of known sequences. 4, record 11, English, - inverted%20polymerase%20chain%20reaction
Record number: 11, Textual support number: 1 OBS
The template target sequence is digested with a suitable restriction enzyme that cuts the template outside the known sequence but in the region of the unknown sequence. PCR is carried out using two primers which anneal to the ends of the known sequence but reversed in their direction with respect to their normal orientation in a normal PCR reaction. This leads to the amplification of the region of the unknown sequence. 4, record 11, English, - inverted%20polymerase%20chain%20reaction
Record 11, French
Record 11, Domaine(s)
- Génétique
Record 11, Main entry term, French
- PCR inverse
1, record 11, French, PCR%20inverse
correct, feminine noun
Record 11, Abbreviations, French
- IPCR 2, record 11, French, IPCR
correct, feminine noun
Record 11, Synonyms, French
Record 11, Textual support, French
Record number: 11, Textual support number: 1 CONT
Le principe même de la mutagénèse dirigée par IPCR nécessite la purification préalable des oligonucléotides utilisés. En effet, la présence de formes n-1, n-2 ou n-x même très minoritaire induirait la formation de mutations supplémentaires par délétion de une, deux ou x bases ors de 12 PCR. 1, record 11, French, - PCR%20inverse
Record number: 11, Textual support number: 2 CONT
Le produit final d'une PCR inverse (amorces tête-bêche) est phosphoryle ligué et transformé. 1, record 11, French, - PCR%20inverse
Record 11, Spanish
Record 11, Textual support, Spanish
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