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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

Heteroduplex is double-stranded DNA in which the two DNA strands do not show perfect base complementarity. When DNA is denatured, the two strands are separated. On renaturation or annealing, complementary DNA strands reassociate and form a homoduplex. However, when there is a mutation in one of the strands then a heteroduplex is formed. The electrophoretic mobility of heteroduplex in polyacrylamide gel is less than that of homoduplex, and they can be detected as an extra slow moving bands. Resolution is better in specially formulated gel matrices (Hydro-link, MDE, etc.). In fragments of under 200 bp insertions, deletions and most single base substitutions are detected. Heteroduplex analysis is mostly used combined with single confirmation analysis (SSCA) on a single gel.  1, record 1, English, - heteroduplex%20analysis

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

Nous présentons les avantages relatifs d'une stratégie de recherche spécifique des mutations qui introduisent une signal d'arrêt prématuré de la synthèse protéique fondée sur le test de troncation des protéines (PTT), par rapport aux techniques classiques de balayage d'un fragment d'ADN ou d'ARN telles que l'électrophorèse en gel de gradient dénaturant (DGGE), l'analyse d'hétéroduplex (HA), l'analyse de conformation de l'ADN en simple brin (SSCA) ou le clivage chimique ou enzymatique de mésappariements (CCM/EMC).  1, record 1, French, - analyse%20d%27h%C3%A9t%C3%A9roduplex

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 DEF

Empleo de la movilidad electroforética del ADN heterodúplex para estimar el grado de no homología entre las secuencias de las dos hebras.  1, record 1, Spanish, - an%C3%A1lisis%20del%20heterod%C3%BAplex

Record number: 1, Textual support number: 1 OBS

La movilidad en gel tiende a reducirse a medida que aumenta la divergencia entre secuencias, dado que el tamaño efectivo de un par de hebras totalmente complementarias es menor que el de una estructura donde la complementación es parcial.  1, record 1, Spanish, - an%C3%A1lisis%20del%20heterod%C3%BAplex

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

A DNA double helix in which the complementary strands are derived from different molecules, so that there may be small regions of mismatching.  2, record 2, English, - heteroduplex

Record number: 2, Textual support number: 1 OBS

They occur naturally during genetic recombination between homologous chromosomes and can be produced artificially in vitro to estimate the degree of similarity between two molecules from different sources.  2, record 2, English, - heteroduplex

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 DEF

Molécule d'ADN partiellement alcaténaire dans laquelle les deux brins ne possèdent pas que des séquences de bases rigoureusement complémentaires. [Définition normalisée par l'AFNOR et reproduite avec son autorisation.]  3, record 2, French, - h%C3%A9t%C3%A9roduplex

Record number: 2, Textual support number: 1 OBS

hétéroduplex : s'emploie également comme adjectif.  4, record 2, French, - h%C3%A9t%C3%A9roduplex

Record number: 2, Textual support number: 2 OBS

hétéroduplex : terme normalisé par l'AFNOR.  5, record 2, French, - h%C3%A9t%C3%A9roduplex

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 DEF

A technique used to identify and isolate the protein product of a cloned gene.  3, record 3, English, - hybrid%2Darrested%20translation

Record number: 3, Textual support number: 1 OBS

The cloned double-stranded DNA is denatured and annealed to a mixture of mRNAs known to contain mRNA [messenger RNA] : DNA hybrids are formed, which renders the relevant mRNA unavailable for translation. Translation of the annealed mixture in an in vitro translation system thus results in the absence of translation product of the test gene. Comparison with the translation products of an untreated sample can then identify the missing protein.  3, record 3, English, - hybrid%2Darrested%20translation

Record 3,  Textual support,  French

Record 3,  Textual support,  Spanish

Record number: 3, Textual support number: 1 DEF

Método utilizado para identificar la proteína o proteínas codificadas por una determinada secuencia de ADN clonado.  1, record 3, Spanish, - traducci%C3%B3n%20impedida%20por%20hibridaci%C3%B3n

Record number: 3, Textual support number: 1 OBS

Una preparación de todo el ARNm [ARN mensajero], que contiene diferentes ARNm, se hibrida con el ADN clonado, de manera que las moléculas de ARNm homólogas al ADN clonado se aparearán con él para formar heterodúplex de ADN/ARN. Las moléculas de ARNm no apareadas se traducen in vitro y el resultado se compara con los productos de la traducción de los ARNm sin tratar.  1, record 3, Spanish, - traducci%C3%B3n%20impedida%20por%20hibridaci%C3%B3n