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The Government of Canada’s terminology and linguistic data bank.
REACCION CADENA POLIMERASA-TRANSCRIPTASA INVERSA [1 record]
Record 1 - internal organization data 2015-04-20
Record 1, English
Record 1, Subject field(s)
- Genetics
- Molecular Biology
Record 1, Main entry term, English
- reverse transcription-polymerase chain reaction
1, record 1, English, reverse%20transcription%2Dpolymerase%20chain%20reaction
correct
Record 1, Abbreviations, English
- RT-PCR 2, record 1, English, RT%2DPCR
correct
Record 1, Synonyms, English
- reverse transcriptase PCR 3, record 1, English, reverse%20transcriptase%20PCR
correct
- RT-PCR 3, record 1, English, RT%2DPCR
correct
- RT-PCR 3, record 1, English, RT%2DPCR
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
It is a technique used in genetic studies that allows the detection and quantification of mRNA [messenger ribonucleic acid] It is a very sensitive method that shows whether or not a specific gene is being expressed in a given sample. RT-PCR is a very important test in the field of Genetically-Modified Organisms (GMO's) because it gives researchers a mechanism to test whether any specific gene is turned on (active) or turned off (inactive). This allows researchers to identify the benefits of genetically-modified organisms with respect to their "natural" counterparts and search for any significant differences in which genes are expressed in the two types of organisms. 3, record 1, English, - reverse%20transcription%2Dpolymerase%20chain%20reaction
Record 1, French
Record 1, Domaine(s)
- Génétique
- Biologie moléculaire
Record 1, Main entry term, French
- méthode PCR-CDNA
1, record 1, French, m%C3%A9thode%20PCR%2DCDNA
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- technique de RT-PCR 2, record 1, French, technique%20de%20RT%2DPCR
correct, feminine noun
- RT-PCR 3, record 1, French, RT%2DPCR
correct
- RT-PCR 3, record 1, French, RT%2DPCR
- méthode RT-PCR 1, record 1, French, m%C3%A9thode%20RT%2DPCR
correct, feminine noun
- technique de l'ADNc-PCR 4, record 1, French, technique%20de%20l%27ADNc%2DPCR
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La PCR et la RT-PCR (reverse transcriptase PCR) permettent la synthèse de millions de copies d'un segment spécifique d'ADN ou d'ARN (après une étape de transcription inverse permettant la synthèse d'un ADNc), grâce à une succession de réactions se faisant à différentes températures : dénaturation de l'ADN cible par rupture des liaisons hydrogène à 94° C, hybridation des amorces sur une séquence complémentaire d'un des brins d'ADN au niveau 3' (entre 37 °C et 68 °C), élongation (à 72 °C le plus souvent) grâce aux dNTP ajoutés dans le milieu réactionnel et à une enzyme thermostable à activité ADN polymérase (Taq polymérase). 3, record 1, French, - m%C3%A9thode%20PCR%2DCDNA
Record number: 1, Textual support number: 1 OBS
Technique de transcription inverse suivie de réaction en chaîne de la polymérase. 5, record 1, French, - m%C3%A9thode%20PCR%2DCDNA
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Genética
- Biología molecular
Record 1, Main entry term, Spanish
- reacción en cadena de la polimerasa con transcriptasa inversa
1, record 1, Spanish, reacci%C3%B3n%20en%20cadena%20de%20la%20polimerasa%20con%20transcriptasa%20inversa
correct, feminine noun
Record 1, Abbreviations, Spanish
- RCP-TI 1, record 1, Spanish, RCP%2DTI
correct, feminine noun
Record 1, Synonyms, Spanish
- reacción en cadena de la polimerasa-transcriptasa inversa 2, record 1, Spanish, reacci%C3%B3n%20en%20cadena%20de%20la%20polimerasa%2Dtranscriptasa%20inversa
correct, feminine noun
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 OBS
[...] la reacción en cadena de la polimerasa-transcriptasa inversa. Se utiliza para la detección y amplificación de ARN, permite estudiar la expresión de determinados genes (su traducción a proteínas). 2, record 1, Spanish, - reacci%C3%B3n%20en%20cadena%20de%20la%20polimerasa%20con%20transcriptasa%20inversa
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