TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

A physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin.  4, record 1, English, - fluorescence%20in%20situ%20hybridization

Record number: 1, Textual support number: 1 CONT

Until recently, even the best chromosomal maps could be used to locate a DNA [deoxyribonucleic acid] fragment only to a region of about 10 Mb, the size of a typical band seen on a chromosome. Improvements in fluorescence in situ hybridization (FISH) methods allow orientation of DNA sequences that lie as close as 2 to 5 Mb.  2, record 1, English, - fluorescence%20in%20situ%20hybridization

Record number: 1, Textual support number: 2 CONT

Fluorescent in situ hybridization on interphase nuclei prepared from normal male fibroblast cultures gave a distance between D9S12 and D9S180 of about one Mb.  3, record 1, English, - fluorescence%20in%20situ%20hybridization

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

Le développement des techniques d’hybridation in situ en fluorescence et la mise au point de systèmes d’imagerie dont les performances répondent aux exigences des cytogénéticiens permettent aujourd’hui la mise en œuvre de méthodes analytiques puissantes, clés de l'investigation efficace du génome et de ses anomalies.  2, record 1, French, - hybridation%20in%20situ%20en%20fluorescence

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 DEF

[Técnica] que utiliza sondas de ADN [ácido desoxirribonucleico] marcadas con fluorescencia para detectar o confirmar anomalías génicas o cromosómicas que generalmente están más allá del poder de resolución de la citogenética de rutina.  1, record 1, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record number: 1, Textual support number: 1 CONT

Fluorescence in situ hybridization (FISH) o hibridación fluorescente in situ es una técnica que combina citogenética clásica con biología molecular. Con esta asociación se logra obtener la información genética manteniendo la estructura de las células o tejidos estudiados. [...] FISH es una herramienta importante en investigación y se incrementa su uso en diagnóstico humano. [...] ejemplos de su aplicación [...]: genética pre y postnatal; diagnóstico, seguimiento y/o pronóstico en cáncer; determinaciones de anomalías en embriones de preimplantación; microbiología; radiobiología; medio ambiente y evolución.  2, record 1, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record number: 1, Textual support number: 1 OBS

FISH por su sigla en inglés.  3, record 1, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

A laser scanning microscopy system that focuses on a single plane of light through a biological substance, allowing high-resolution, three-dimensional images to be built up.  4, record 2, English, - confocal%20laser%20scanning%20microscopy

Record number: 2, Textual support number: 1 CONT

Laser Scanning Confocal Microscopy (LSCM, also referred to as CSLM, Confocal Scanning Laser Microscopy) is now established as a valuable tool for obtaining high resolution images and 3-D reconstructions of a variety of biological specimens. In LSCM, a laser light beam is expanded to make optimal use of the optics in the objective. Through a x-y deflection mechanism this beam is turned into a scanning beam, focussed to a small spot by an objective lens onto a fluorescent specimen. The mixture of reflected light and emitted fluorescent light is captured by the same objective and (after conversion into a static beam by the x-y scanner device) is focused onto a photodetector (photomultiplier) via a dichroic mirror (beam splitter). The reflected light is deviated by the dichroic mirror while the emitted fluorescent light passes through in the direction of the photomultiplier. A confocal aperture (pinhole) is placed in front of the photodetector, such that the fluorescent light (not the reflected light!) from points on the specimen that are not within the focal plane (the so called out-of-focus light) where the laser beam was focussed will be largly obstructed by the pinhole. In this way, out-of-focus information (both above and below the focal plane) is greatly reduced.  2, record 2, English, - confocal%20laser%20scanning%20microscopy

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

Principe de la microscopie confocale à balayage laser (MCBL). Le rayon laser excitateur pénètre dans l’échantillon préalablement marqué par des fluorochromes [...], auparavant répertoriés et choisis en fonction de leurs propriétés à se fixer sur des molécules particulières d’une structure ou d’un objet d’intérêt. Lors de l’impact optique, il y a émission de rayons lumineux provenant de différents plans de la préparation. Grâce à un diaphragme variable («trou d’aiguille» - pinhole), il est possible de sélectionner les rayons émis par un seul plan de préparation et d’éliminer le signal provenant d’autres plans. Les rayons réfléchis sont filtrés en fonction de leur longueurs d’onde puis détectés par des photo-multiplicateurs. Le signal reçu est enfin converti en signal numérique, contribuant à la création d’une image [...]  2, record 2, French, - microscopie%20confocale%20%C3%A0%20balayage%20laser

Record number: 2, Textual support number: 1 OBS

Tous les champs d’application de la microscopie confocale à balayage laser : localisations intracellulaires par fluorescence(simple et double marquage), reconstitution 3D par fluorescence de structures cellulaires, hybridation in situ, cinétique de processus dans les cellules vivantes, états de surface de matériaux divers.  3, record 2, French, - microscopie%20confocale%20%C3%A0%20balayage%20laser

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 CONT

Breast cancer progression is determined by a complex pattern of multiple genetic aberrations the association of which with patient prognosis is unknown. In this study, we have undertaken a genome-wide screening to detect genetic changes associated with clinical outcome in node-negative breast cancer. Comparative genomic hybridization was used to screen for DNA sequence gains and losses across all human chromosomes in 23 tumors ...  1, record 3, English, - genomic%20hybridization

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 CONT

La séquence FISH(hybridation en fluorescence in situ) et le développement des techniques fondées sur son principe sont en train de modifier radicalement les méthodes d’analyse de la génétique. Parmi celles-ci, l'hybridation génomique comparative(CGH) constitue un outil performant d’exploration du génome.  1, record 3, French, - hybridation%20g%C3%A9nomique%20comparative

Record number: 3, Textual support number: 2 CONT

La CGH est une méthode permettant d’évaluer une modification du nombre de copies d’ADN dans un génome pathologique [...] Son principe est fondé sur la compétition, lors de l’hybridation, entre un ADN génomique normal et un ADN génomique pathologique.  1, record 3, French, - hybridation%20g%C3%A9nomique%20comparative

Record 3,  Textual support,  Spanish