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HYBRIDATION MOLECULAIRE [15 records]
Record 1 - internal organization data 2020-08-21
Record 1, English
Record 1, Subject field(s)
- Molecular Biology
- Biotechnology
Record 1, Main entry term, English
- stringency
1, record 1, English, stringency
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
The condition with regard to temperature, ionic strength, and the presence of certain organic solvents ... under which nucleic acid hybridizations are carried out. 2, record 1, English, - stringency
Record number: 1, Textual support number: 1 CONT
With conditions of high stringency, pairing will occur only between nucleic acid fragments that have a high frequency of complementary base sequences. Conditions of weaker stringency must be used if the nucleic acids come from organisms that are genetically diverse. 3, record 1, English, - stringency
Record 1, French
Record 1, Domaine(s)
- Biologie moléculaire
- Biotechnologie
Record 1, Main entry term, French
- stringence
1, record 1, French, stringence
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Le terme «stringence» est le terme consacré en biologie moléculaire pour qualifier le degré de rigueur des conditions d’hybridation qui régissent la spécificité de l'hybridation. 1, record 1, French, - stringence
Record 1, Spanish
Record 1, Textual support, Spanish
Record 2 - internal organization data 2013-11-15
Record 2, English
Record 2, Subject field(s)
- Genetics
Record 2, Main entry term, English
- nitrocellulose filter
1, record 2, English, nitrocellulose%20filter
correct
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, Textual support, English
Record number: 2, Textual support number: 1 DEF
A flat sheet of paperlike material made from cellulose nitrate, a nitric acid ester of cellulose. Cellulose nitrate binds DNA strongly and can be permanently fixed by baking at 80°C. 1, record 2, English, - nitrocellulose%20filter
Record 2, French
Record 2, Domaine(s)
- Génétique
Record 2, Main entry term, French
- filtre de nitrocellulose
1, record 2, French, filtre%20de%20nitrocellulose
correct, masculine noun
Record 2, Abbreviations, French
Record 2, Synonyms, French
- membrane de nitrocellulose 2, record 2, French, membrane%20de%20nitrocellulose
correct, feminine noun
Record 2, Textual support, French
Record number: 2, Textual support number: 1 DEF
Feuille, composée de nitrate de cellulose, utilisée pour effectuer des analyses de polynucléotides. On s’en sert notamment de support d’hybridation moléculaire pour les southern ou northern blots, les molécules d’ADN s’y fixant fortement. 3, record 2, French, - filtre%20de%20nitrocellulose
Record 2, Spanish
Record 2, Textual support, Spanish
Record 3 - internal organization data 2013-11-15
Record 3, English
Record 3, Subject field(s)
- Genetics
Record 3, Main entry term, English
- somatic cell genetics
1, record 3, English, somatic%20cell%20genetics
correct
Record 3, Abbreviations, English
Record 3, Synonyms, English
Record 3, Textual support, English
Record number: 3, Textual support number: 1 DEF
The study of genetic phenomena in cultured somatic cells. Its range is broad, encompassing the expression of mutant cells in culture; gene mapping; the study of differentiated function and malignancy; and the factors controlling differentiation in culture, cloning, and hybridization. 2, record 3, English, - somatic%20cell%20genetics
Record 3, French
Record 3, Domaine(s)
- Génétique
Record 3, Main entry term, French
- génétique somatique
1, record 3, French, g%C3%A9n%C3%A9tique%20somatique
correct, feminine noun
Record 3, Abbreviations, French
Record 3, Synonyms, French
Record 3, Textual support, French
Record number: 3, Textual support number: 1 CONT
La génétique somatique a clairement démontré la fécondité des recherches concernant l'organisation, l'expression et la régulation des gènes chez de nombreuses espèces, y compris l'homme. La possibilité de manipuler et d’introduire du matériel génétique dans des cellules en culture grâce à l'hybridation cellulaire nous permet maintenant d’étudier l'expression des gènes au niveau de la cellule in vitro, mais aussi in vivo dans un organisme entier. Ces méthodes de génétique somatique, ainsi que leur développement moléculaire, seront des outils très importants de la biologie de demain. 2, record 3, French, - g%C3%A9n%C3%A9tique%20somatique
Record 3, Spanish
Record 3, Textual support, Spanish
Record 4 - internal organization data 2012-08-31
Record 4, English
Record 4, Subject field(s)
- Genetics
Record 4, Main entry term, English
- genetic distance 1, record 4, English, genetic%20distance
Record 4, Abbreviations, English
Record 4, Synonyms, English
Record 4, Textual support, English
Record number: 4, Textual support number: 1 DEF
A measure of the number of allelic substitutions per locus that have occurred during the separate evolution of two populations or species. 2, record 4, English, - genetic%20distance
Record 4, French
Record 4, Domaine(s)
- Génétique
Record 4, Main entry term, French
- distance génétique
1, record 4, French, distance%20g%C3%A9n%C3%A9tique
correct, feminine noun
Record 4, Abbreviations, French
Record 4, Synonyms, French
Record 4, Textual support, French
Record number: 4, Textual support number: 1 DEF
Degré de parenté entre des génomes différents. 1, record 4, French, - distance%20g%C3%A9n%C3%A9tique
Record number: 4, Textual support number: 1 OBS
En génétique moléculaire, la distance génétique est estimée par le pourcentage d’homologie structurale que présentent deux séquences(par hybridation moléculaire et/ou par comparaison des séquences nucléotidiques). 1, record 4, French, - distance%20g%C3%A9n%C3%A9tique
Record 4, Spanish
Record 4, Campo(s) temático(s)
- Genética
Record 4, Main entry term, Spanish
- distancia genética
1, record 4, Spanish, distancia%20gen%C3%A9tica
correct, feminine noun
Record 4, Abbreviations, Spanish
Record 4, Synonyms, Spanish
Record 4, Textual support, Spanish
Record number: 4, Textual support number: 1 DEF
Medida de la semejanza genética entre dos poblaciones. 1, record 4, Spanish, - distancia%20gen%C3%A9tica
Record number: 4, Textual support number: 1 CONT
Se calcula sobre la base de la variación en una combinación de caracteres fenotípicos, frecuencias alélicas o secuencias de ADN. Por ejemplo, la distancia genética entre dos poblaciones que tienen las mismas frecuencias alélicas para un determinado locus, y que se mide solamente en ese locus, es cero. 1, record 4, Spanish, - distancia%20gen%C3%A9tica
Record 5 - internal organization data 2012-08-28
Record 5, English
Record 5, Subject field(s)
- Genetics
- Molecular Biology
Record 5, Main entry term, English
- reverse dot blot
1, record 5, English, reverse%20dot%20blot
correct
Record 5, Abbreviations, English
Record 5, Synonyms, English
- reverse dot-blot 2, record 5, English, reverse%20dot%2Dblot
correct
Record 5, Textual support, English
Record number: 5, Textual support number: 1 CONT
Dot blots and reverse dot blots are techniques where PCR products are hybridized with allele specific oligonucleotides (ASOs) for the purpose of scanning for mutations, detecting RNA, cancer, or pathogen 1, record 5, English, - reverse%20dot%20blot
Record 5, French
Record 5, Domaine(s)
- Génétique
- Biologie moléculaire
Record 5, Main entry term, French
- buvardage en tache inverse
1, record 5, French, buvardage%20en%20tache%20inverse
correct, masculine noun
Record 5, Abbreviations, French
Record 5, Synonyms, French
- hybridation sur tache inverse 2, record 5, French, hybridation%20sur%20tache%20inverse
correct, feminine noun
- dot-blot inverse 3, record 5, French, dot%2Dblot%20inverse
avoid, masculine noun
Record 5, Textual support, French
Record number: 5, Textual support number: 1 CONT
Les produits peuvent selon la technique dite du dot-blot inverse, être hybridés sur une membrane de nylon avec diverses sondes d’ADN porteuses d’un maqueur radioactif ou d’une enzyme. 3, record 5, French, - buvardage%20en%20tache%20inverse
Record number: 5, Textual support number: 1 OBS
Hybridation sur tache : Méthode d’hybridation moléculaire entre un acide nucléique directement absorbé sous forme de tache sur un filtre 4, record 5, French, - buvardage%20en%20tache%20inverse
Record 5, Spanish
Record 5, Textual support, Spanish
Record 6 - internal organization data 2012-04-18
Record 6, English
Record 6, Subject field(s)
- Genetics
Record 6, Main entry term, English
- hybridization
1, record 6, English, hybridization
correct
Record 6, Abbreviations, English
Record 6, Synonyms, English
- nucleic acid hybridization 2, record 6, English, nucleic%20acid%20hybridization
correct
- molecular hybridization 3, record 6, English, molecular%20hybridization
correct
Record 6, Textual support, English
Record number: 6, Textual support number: 1 DEF
A process in which a strand of nucleic acid joins with a complementary strand through base pairing. 4, record 6, English, - hybridization
Record number: 6, Textual support number: 1 OBS
The technique is used to locate or identify nucleotide sequences and to establish the effective transfer of nucleic material to a new host. 4, record 6, English, - hybridization
Record 6, French
Record 6, Domaine(s)
- Génétique
Record 6, Main entry term, French
- hybridation moléculaire
1, record 6, French, hybridation%20mol%C3%A9culaire
correct, feminine noun, standardized
Record 6, Abbreviations, French
Record 6, Synonyms, French
- hybridation 2, record 6, French, hybridation
correct, feminine noun
Record 6, Textual support, French
Record number: 6, Textual support number: 1 DEF
Appariement de chaînes d’acides nucléiques complémentaires simple brin (ADN et ARN). [Définition normalisée par l’AFNOR et reproduite avec son autorisation.] 3, record 6, French, - hybridation%20mol%C3%A9culaire
Record number: 6, Textual support number: 1 OBS
La formation de régions double brin est l’indication d’une complémentarité de séquence. Le résultat de cet appariement est un hybride ADN-ARN. [Observation reproduite avec l’autorisation de l’AFNOR.] 3, record 6, French, - hybridation%20mol%C3%A9culaire
Record number: 6, Textual support number: 2 OBS
hybridation moléculaire : terme normalisé par l'AFNOR. 4, record 6, French, - hybridation%20mol%C3%A9culaire
Record 6, Spanish
Record 6, Campo(s) temático(s)
- Genética
Record 6, Main entry term, Spanish
- hibridación de ácidos nucleicos
1, record 6, Spanish, hibridaci%C3%B3n%20de%20%C3%A1cidos%20nucleicos
correct, feminine noun
Record 6, Abbreviations, Spanish
Record 6, Synonyms, Spanish
Record 6, Textual support, Spanish
Record 7 - internal organization data 2003-05-22
Record 7, English
Record 7, Subject field(s)
- Genetics
Record 7, Main entry term, English
- Northern blot
1, record 7, English, Northern%20blot
correct
Record 7, Abbreviations, English
Record 7, Synonyms, English
- Northern blotting 2, record 7, English, Northern%20blotting
correct
- Northern blot method 3, record 7, English, Northern%20blot%20method
correct, less frequent
Record 7, Textual support, English
Record number: 7, Textual support number: 1 DEF
After electrophoresis, a technique for locating specific RNA fragments after their transfer to nitrocellulose filters. 4, record 7, English, - Northern%20blot
Record number: 7, Textual support number: 1 OBS
The RNA is then hybridized to nick-translated probes. 4, record 7, English, - Northern%20blot
Record number: 7, Textual support number: 2 OBS
The technique was named by opposition to the Southern blot technique which uses DNA fragments instead of RNA fragments. 4, record 7, English, - Northern%20blot
Record 7, French
Record 7, Domaine(s)
- Génétique
Record 7, Main entry term, French
- transfert de Northern
1, record 7, French, transfert%20de%20Northern
correct, masculine noun
Record 7, Abbreviations, French
Record 7, Synonyms, French
- transfert Northern 2, record 7, French, transfert%20Northern
correct, masculine noun
- transfert de type Northern 3, record 7, French, transfert%20de%20type%20Northern
correct, masculine noun
- Northern blot 4, record 7, French, Northern%20blot
avoid, see observation
Record 7, Textual support, French
Record number: 7, Textual support number: 1 DEF
Technique de la génétique moléculaire qui consiste à détecter des fragments d’ARN transférés sur filtres de nitrocellulose par leur hybridation à des séquences identiques radioactives ou sondes. 5, record 7, French, - transfert%20de%20Northern
Record number: 7, Textual support number: 1 OBS
Bien que Northern blot soit en usage dans la communauté scientifique francophone, il est préférable d’utiliser les termes français mentionnés sur cette fiche. 4, record 7, French, - transfert%20de%20Northern
Record 7, Spanish
Record 7, Campo(s) temático(s)
- Genética
Record 7, Main entry term, Spanish
- técnica de Northern blot
1, record 7, Spanish, t%C3%A9cnica%20de%20Northern%20blot
feminine noun
Record 7, Abbreviations, Spanish
Record 7, Synonyms, Spanish
Record 7, Textual support, Spanish
Record number: 7, Textual support number: 1 DEF
Técnica de transferencia por gravedad de bandas de ARN separado por electroforesis a una membrana de nilón o nitrocelulosa. 2, record 7, Spanish, - t%C3%A9cnica%20de%20Northern%20blot
Record 8 - internal organization data 1999-10-22
Record 8, English
Record 8, Subject field(s)
- Genetics
Record 8, Main entry term, English
- chromosomal genetics
1, record 8, English, chromosomal%20genetics
proposal
Record 8, Abbreviations, English
Record 8, Synonyms, English
Record 8, Textual support, English
Record 8, French
Record 8, Domaine(s)
- Génétique
Record 8, Main entry term, French
- génétique chromosomique
1, record 8, French, g%C3%A9n%C3%A9tique%20chromosomique
correct, feminine noun
Record 8, Abbreviations, French
Record 8, Synonyms, French
Record 8, Textual support, French
Record number: 8, Textual support number: 1 CONT
Génétique chromosomique et génétique moléculaire : une hybridation réussie. 1, record 8, French, - g%C3%A9n%C3%A9tique%20chromosomique
Record 8, Spanish
Record 8, Textual support, Spanish
Record 9 - internal organization data 1997-07-16
Record 9, English
Record 9, Subject field(s)
- Genetics
Record 9, Main entry term, English
- molecular cytogenetics
1, record 9, English, molecular%20cytogenetics
correct
Record 9, Abbreviations, English
Record 9, Synonyms, English
Record 9, Textual support, English
Record number: 9, Textual support number: 1 CONT
The LBNL/UCSF Resource for Molecular Cytogenetics has been created to facilitate the application of molecular cytogenetics in clinical and biological studies. Work is being pursued in three areas: Development and application of improved hybridization technology, selection of probes optimized for use in fluorescence in situ hybridization (FISH), and development of digital imaging microscopy. 1, record 9, English, - molecular%20cytogenetics
Record number: 9, Textual support number: 1 OBS
Resource for Molecular Cytogenetics Cancer Center, University of California, San Francisco Lawrence Berkeley National Laboratory, Berkeley California. 2, record 9, English, - molecular%20cytogenetics
Record 9, French
Record 9, Domaine(s)
- Génétique
Record 9, Main entry term, French
- cytogénétique moléculaire
1, record 9, French, cytog%C3%A9n%C3%A9tique%20mol%C3%A9culaire
correct, feminine noun
Record 9, Abbreviations, French
Record 9, Synonyms, French
Record 9, Textual support, French
Record number: 9, Textual support number: 1 CONT
Cependant deux nouvelles techniques de cytogénétique moléculaire utilisant la FISH ont été développées au cours des cinq dernières années : l'hybridation génomique comparative et le caryotype spectral. 1, record 9, French, - cytog%C3%A9n%C3%A9tique%20mol%C3%A9culaire
Record 9, Spanish
Record 9, Textual support, Spanish
Record 10 - internal organization data 1997-05-20
Record 10, English
Record 10, Subject field(s)
- Cytology
Record 10, Main entry term, English
- laser scanning cytometry
1, record 10, English, laser%20scanning%20cytometry
correct
Record 10, Abbreviations, English
- LSC 1, record 10, English, LSC
correct
Record 10, Synonyms, English
Record 10, Textual support, English
Record number: 10, Textual support number: 1 CONT
DNA ploidy analysis by laser scanning cytometry (LSC) in colorectal cancers and comparison with flow cytometry. 1, record 10, English, - laser%20scanning%20cytometry
Record number: 10, Textual support number: 2 CONT
Analysis by laser scanning cytometry of Bacillus thuringiensis toxin binding sites on Chilo suppressalis. 2, record 10, English, - laser%20scanning%20cytometry
Record 10, French
Record 10, Domaine(s)
- Cytologie
Record 10, Main entry term, French
- cytométrie à balayage laser
1, record 10, French, cytom%C3%A9trie%20%C3%A0%20balayage%20laser
correct, feminine noun
Record 10, Abbreviations, French
Record 10, Synonyms, French
Record 10, Textual support, French
Record number: 10, Textual support number: 1 CONT
La société Chemunex(Maisons-Alfort) développe une méthode rapide et sensible, qui associe l'hybridation moléculaire sur membranes filtrantes et la cytométrie à balayage laser(système dit ChemScan). 1, record 10, French, - cytom%C3%A9trie%20%C3%A0%20balayage%20laser
Record number: 10, Textual support number: 1 OBS
Détection de bactéries pathogènes alimentaires. 2, record 10, French, - cytom%C3%A9trie%20%C3%A0%20balayage%20laser
Record 10, Spanish
Record 10, Textual support, Spanish
Record 11 - internal organization data 1996-11-05
Record 11, English
Record 11, Subject field(s)
- Biotechnology
- Crop Protection
Record 11, Main entry term, English
- digoxygenin-labeled hybridization assay 1, record 11, English, digoxygenin%2Dlabeled%20hybridization%20assay
Record 11, Abbreviations, English
- dig-labeled hybridization assay 2, record 11, English, dig%2Dlabeled%20hybridization%20assay
Record 11, Synonyms, English
Record 11, Textual support, English
Record number: 11, Textual support number: 1 CONT
A dig-labeled hybridization assay was developed for the detection of crown gall disease (Agrobacterium vitis), providing a highly sensitive, specific, and low-cost alternative to polymerase chain reaction assays. 1, record 11, English, - digoxygenin%2Dlabeled%20hybridization%20assay
Record 11, French
Record 11, Domaine(s)
- Biotechnologie
- Protection des végétaux
Record 11, Main entry term, French
- épreuve d'hybridation par marquage à la digoxygénine
1, record 11, French, %C3%A9preuve%20d%27hybridation%20par%20marquage%20%C3%A0%20la%20digoxyg%C3%A9nine
feminine noun
Record 11, Abbreviations, French
Record 11, Synonyms, French
- test d'hybridation par marquage à la digoxygénine 1, record 11, French, test%20d%27hybridation%20par%20marquage%20%C3%A0%20la%20digoxyg%C3%A9nine
masculine noun
Record 11, Textual support, French
Record number: 11, Textual support number: 1 OBS
L'hybridation consiste à mettre en évidence, grâce au marquage, l'appariement d’une molécule d’acide nucléique sonde avec une molécule d’acide nucléique cible dont la séquence est complémentaire. La mise au point de techniques de marquage non radioactif performantes(sondes froides) permet d’envisager une large application de l'hybridation moléculaire pour la détection des agents phytopathogènes. 2, record 11, French, - %C3%A9preuve%20d%27hybridation%20par%20marquage%20%C3%A0%20la%20digoxyg%C3%A9nine
Record number: 11, Textual support number: 2 OBS
De nombreux haptènes ont été employés avec succès pour préparer des sondes non radioactives : biotine, éthidium, N-2-acétyl-aminofluorène(AAF), triphénol, sulfone, digoxygénine... Après hybridation, la sonde est détectée grâce à une protéine marquée spécifique de cet haptène. [...] Il existe plusieurs schémas réactionnels disponibles pour réaliser un test d’hybridation moléculaire. 2, record 11, French, - %C3%A9preuve%20d%27hybridation%20par%20marquage%20%C3%A0%20la%20digoxyg%C3%A9nine
Record 11, Spanish
Record 11, Textual support, Spanish
Record 12 - internal organization data 1996-10-23
Record 12, English
Record 12, Subject field(s)
- Genetics
Record 12, Main entry term, English
- plaque lift
1, record 12, English, plaque%20lift
correct
Record 12, Abbreviations, English
Record 12, Synonyms, English
Record 12, Textual support, English
Record number: 12, Textual support number: 1 CONT
[In the colony blot technique] the molecules (usually DNA) are from colonies of bacteria or yeast growing on a bacteriological plate. A variation (called the plaque lift can also be used for viruses). 2, record 12, English, - plaque%20lift
Record 12, French
Record 12, Domaine(s)
- Génétique
Record 12, Main entry term, French
- transfert de plages
1, record 12, French, transfert%20de%20plages
correct, masculine noun
Record 12, Abbreviations, French
Record 12, Synonyms, French
Record 12, Textual support, French
Record number: 12, Textual support number: 1 DEF
Transfert de phages présents dans des plages de lyse d’une boîte de Petri sur un filtre avant de procéder à une hybridation moléculaire de leur matériel génétique. 1, record 12, French, - transfert%20de%20plages
Record 12, Spanish
Record 12, Textual support, Spanish
Record 13 - internal organization data 1995-07-24
Record 13, English
Record 13, Subject field(s)
- Genetics
Record 13, Main entry term, English
- colony lift
1, record 13, English, colony%20lift
correct
Record 13, Abbreviations, English
Record 13, Synonyms, English
Record 13, Textual support, English
Record 13, French
Record 13, Domaine(s)
- Génétique
Record 13, Main entry term, French
- transfert de colonies
1, record 13, French, transfert%20de%20colonies
correct, masculine noun
Record 13, Abbreviations, French
Record 13, Synonyms, French
Record 13, Textual support, French
Record number: 13, Textual support number: 1 DEF
Transfert de colonies de cellules d’une boîte de Petri sur un filtre avant de procéder à une hybridation moléculaire de leur matériel génétique. 1, record 13, French, - transfert%20de%20colonies
Record 13, Spanish
Record 13, Textual support, Spanish
Record 14 - internal organization data 1990-02-21
Record 14, English
Record 14, Subject field(s)
- Genetics
Record 14, Main entry term, English
- cruciform
1, record 14, English, cruciform
correct
Record 14, Abbreviations, English
Record 14, Synonyms, English
Record 14, Textual support, English
Record number: 14, Textual support number: 1 DEF
The structure produced at inverted repeats of DNA if the repeated sequence pairs with its complement on the same strand (instead of with its regular partner in the other strand of the duplex). 1, record 14, English, - cruciform
Record 14, French
Record 14, Domaine(s)
- Génétique
Record 14, Main entry term, French
- cruciforme
1, record 14, French, cruciforme
correct, feminine noun
Record 14, Abbreviations, French
Record 14, Synonyms, French
Record 14, Textual support, French
Record number: 14, Textual support number: 1 DEF
Structure produite par des répétitions inversées d’une séquence d’ADN, lorsque ces deux séquences s’apparient par complémentarité(hybridation moléculaire) sur le même brin(au lieu de s’apparier avec leur partenaire normal du brin en vis à vis dans la double hélice). 1, record 14, French, - cruciforme
Record 14, Spanish
Record 14, Textual support, Spanish
Record 15 - internal organization data 1988-05-16
Record 15, English
Record 15, Subject field(s)
- Genetics
Record 15, Main entry term, English
- total genomic DNA
1, record 15, English, total%20genomic%20DNA
proposal
Record 15, Abbreviations, English
Record 15, Synonyms, English
Record 15, Textual support, English
Record 15, French
Record 15, Domaine(s)
- Génétique
Record 15, Main entry term, French
- ADN génomique total
1, record 15, French, ADN%20g%C3%A9nomique%20total
correct, masculine noun
Record 15, Abbreviations, French
Record 15, Synonyms, French
Record 15, Textual support, French
Record number: 15, Textual support number: 1 CONT
[Dans la détection directe d’une mutation ponctuelle], des procédés ont été décrits, qui permettent de détecter(toujours par hybridation moléculaire) les mutations ponctuelles dans l'ADN génomique total, sans digestion enzymatique préalable(par "dot blot") ou sans que l'on ai besoin de reconnaître la nature de la mutation. 1, record 15, French, - ADN%20g%C3%A9nomique%20total
Record 15, Spanish
Record 15, Textual support, Spanish
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