TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

Examination of whole cell lipid profiles for PM1 [Methylibium petroleiphilum strain] and YM1 [Bacillus sp. strain] indicates that they are not identical strains. Thermal gradient gel electrophoresis (TGGE) analysis of 16S rRNA gene fragments from the two strains, however, showed no difference between them. 16S rDNA sequence analysis of strains PM1 and YM1 revealed that they are both most closely related to the genus Sphingomonas.  2, record 1, English, - thermal%20gradient%20gel%20electrophoresis

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

L'analyse de fragments obtenus après digestion de l'ADN ribosomal amplifié par des enzymes de restriction(ARDRA, amplified ribosomal DNA restriction analysis), la séparation des fragments amplifiés par électrophorèse sur gel avec gradient de dénaturation(DGGE, TGGE : thermal gradient gel electrophoresis) permettent de caractériser les communautés microbiennes par leur signature moléculaire.  3, record 1, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20en%20gradient%20de%20temp%C3%A9rature

Record number: 1, Textual support number: 1 OBS

TGGE est le sigle anglais.  4, record 1, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20en%20gradient%20de%20temp%C3%A9rature

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 DEF

Método para separar fragmentos de ADN según su movilidad bajo condiciones que favorecen progresivamente la desnaturalización por calor.  1, record 1, Spanish, - electroforesis%20en%20gel%20de%20gradiente%20t%C3%A9rmico

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

High-resolution HLA-DRB [human leucocyte antigen-5,6-dichloro-furanosyl-benzimidazole riboside] typing is required for bone marrow transplantation between unrelated donors and recipients and also for identification of novel HLA-DRB alleles. Here we describe a method for the unambiguous identification of HLA-DRB alleles using the polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct sequencing.  1, record 2, English, - denaturing%20gradient%20gel%20electrophoresis

Record 2,  Textual support,  French

Record 2,  Textual support,  Spanish

Record number: 2, Textual support number: 1 DEF

Método de electroforesis para separar fragmentos de ADN del mismo tamaño en función de su secuencia.  1, record 2, Spanish, - electroforesis%20en%20gel%20desnaturalizante%20en%20gradiente

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 CONT

Heteroduplex is double-stranded DNA in which the two DNA strands do not show perfect base complementarity. When DNA is denatured, the two strands are separated. On renaturation or annealing, complementary DNA strands reassociate and form a homoduplex. However, when there is a mutation in one of the strands then a heteroduplex is formed. The electrophoretic mobility of heteroduplex in polyacrylamide gel is less than that of homoduplex, and they can be detected as an extra slow moving bands. Resolution is better in specially formulated gel matrices (Hydro-link, MDE, etc.). In fragments of under 200 bp insertions, deletions and most single base substitutions are detected. Heteroduplex analysis is mostly used combined with single confirmation analysis (SSCA) on a single gel.  1, record 3, English, - heteroduplex%20analysis

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 CONT

Nous présentons les avantages relatifs d’une stratégie de recherche spécifique des mutations qui introduisent une signal d’arrêt prématuré de la synthèse protéique fondée sur le test de troncation des protéines(PTT), par rapport aux techniques classiques de balayage d’un fragment d’ADN ou d’ARN telles que l'électrophorèse en gel de gradient dénaturant(DGGE), l'analyse d’hétéroduplex(HA), l'analyse de conformation de l'ADN en simple brin(SSCA) ou le clivage chimique ou enzymatique de mésappariements(CCM/EMC).  1, record 3, French, - analyse%20d%26rsquo%3Bh%C3%A9t%C3%A9roduplex

Record 3,  Textual support,  Spanish

Record number: 3, Textual support number: 1 DEF

Empleo de la movilidad electroforética del ADN heterodúplex para estimar el grado de no homología entre las secuencias de las dos hebras.  1, record 3, Spanish, - an%C3%A1lisis%20del%20heterod%C3%BAplex

Record number: 3, Textual support number: 1 OBS

La movilidad en gel tiende a reducirse a medida que aumenta la divergencia entre secuencias, dado que el tamaño efectivo de un par de hebras totalmente complementarias es menor que el de una estructura donde la complementación es parcial.  1, record 3, Spanish, - an%C3%A1lisis%20del%20heterod%C3%BAplex

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 CONT

Chemical Mismatch Cleavage (CMC) is often referred to as Chemical Cleavage of Mismatch (CCM), Amplification Mismatch Detection (AMD), Hydroxylamine Osmium tetroxide method (HOT); and is a method for searching for the presence of unknown mutations.  2, record 4, English, - chemical%20mismatch%20cleavage

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Nous présentons les avantages relatifs d’une stratégie de recherche spécifique des mutations qui introduisent un signal d’arrêt prématuré de la synthèse protéique fondée sur le test de troncation des protéines(PTT), par rapport aux techniques classiques de balayage d’un fragment d’ADN ou d’ARN telles que l'électrophorèse en gel de gradient dénaturant(DGGE), l'analyse d’hétéroduplex(HA), l'analyse de conformation de l'ADN en simple brin(SSCA) ou le clivage chimique ou enzymatique de mésappariements(CCM/EMC).  1, record 4, French, - clivage%20chimique%20de%20m%C3%A9sappariements

Record 4,  Textual support,  Spanish

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 CONT

Currently, the multiplex PCR products are frequently used in efforts trying to identify point mutations. The techniques applied include single-strand conformation analysis (SSCA), hetero-duplex analysis (HD) and double-strand conformation analysis (DSCA). With these technologies, polymorphisms can be detected in frequent and in rare polymorphisms.  3, record 5, English, - single%20strand%20conformation%20analysis

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 CONT

Nous présentons les avantages relatifs d’une stratégie de recherche spécifique des mutations qui introduisent une signal d’arrêt prématuré de la synthèse protéique fondée sur le test de troncation des protéines(PTT), par rapport aux techniques classiques de balayage d’un fragment d’ADN ou d’ARN telles que l'électrophorèse en gel de gradient dénaturant(DGGE), l'analyse d’hétéroduplex(HA), l'analyse de conformation de l'ADN en simple brin(SSCA) ou le clivage chimique ou enzymatique de mésappariements(CCM/EMC).  1, record 5, French, - analyse%20de%20conformation%20de%20l%26rsquo%3BADN%20en%20simple%20brin

Record 5,  Textual support,  Spanish

Record 6,  Textual support,  English

Record number: 6, Textual support number: 1 CONT

A number of methods are available that can detect known DNA mutations e.g. Allele specific amplification/hybridisation(ASA/H), Oligonucleotide ligation assay (OLA), and mini-sequencing just to mention a few. A common drawback for all of these methods is that they only detect mutations known in advance i.e. mutations outside the small area of detection will go undetected. If the position or nature of mutations is unknown then one must define the type of information required :Screening: Is there a mutation or not? SSCP, DGGE ... How many and where are they localised? EMC (Enzymatic Mutation Cleavage)...and CCM (Chemical Cleavage by Mismatch).  3, record 6, English, - enzymatic%20mismatch%20cleavage

Record 6,  Textual support,  French

Record number: 6, Textual support number: 1 CONT

Nous présentons les avantages relatifs d’une stratégie de recherche spécifique des mutations qui introduisent une signal d’arrêt prématuré de la synthèse protéique fondée sur le test de troncation des protéines(PTT), par rapport aux techniques classiques de balayage d’un fragment d’ADN ou d’ARN telles que l'électrophorèse en gel de gradient dénaturant(DGGE), l'analyse d’hétéroduplex(HA), l'analyse de conformation de l'ADN en simple brin(SSCA) ou le clivage chimique ou enzymatique de mésappariements(CCM/EMC).  1, record 6, French, - clivage%20enzymatique%20de%20m%C3%A9sappariements

Record 6,  Textual support,  Spanish

Record 7,  Textual support,  English

Record number: 7, Textual support number: 1 CONT

Only amplified ribosomal DNA restriction analysis (ARDRA) was suitable in recognizing different G. vaginalis genotypes. At least 3-4 genotypes were identified with different restriction enzymes, some of which showed a prevalent distribution in certain of the centers from which they were collected. Although in this study no correlation was found between bacterial vaginosis and any of the genotypes identified, the ARDRA method could prove to be a useful tool for studying the etiopathology and epidemiology of G. vaginalis.  3, record 7, English, - amplified%20ribosomal%20DNA%20restriction%20analysis

Record 7,  Textual support,  French

Record number: 7, Textual support number: 1 CONT

L'analyse des fragments obtenus après digestion de l'ADN ribosomal amplifié par des enzymes de restriction(ARDRA, amplified ribosomal DNA restriction analysis), la séparation des fragments amplifiés par électrophorèse sur gel avec gradient de dénaturation(DGGE, TGGE, denaturating of thermal gradient gel electrophoresis) permettant de caractériser les communautés microbiennes par la signature moléculaire.  1, record 7, French, - ADN%20ribosomal%20amplifi%C3%A9%20par%20des%20enzymes%20de%20restriction

Record 7,  Textual support,  Spanish