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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

Any of the individual polynucleotide sequences produced by digestion of deoxyribonucleic acid with a restriction endonuclease.  2, record 1, English, - restriction%20fragment

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

Nos sujets sont la caractérisation de polymorphismes de fragment de restriction de l'ADN(RFLP) associés au phénotype de troubles affectifs bipolaires, l'identification du gène responsable du glaucome juvénile à angle ouvert, l'identification de l'enzyme responsable dans la dystrophie myotonique de Steinert, tout comme l'hybridation «in situ» chromosomique pour des fins diagnostiques et de recherche [...]  2, record 1, French, - fragment%20de%20restriction

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 DEF

Molécula acortada de ADN generada por la rotura de una molécula mayor por la acción de una o más endonucleasas de restricción.  1, record 1, Spanish, - fragmento%20de%20restricci%C3%B3n

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

A cloning vehicle which has cleavage sites for two or more restriction endonucleases, thus defining a fragment of DNA which can be removed and replaced by foreign DNA.  2, record 2, English, - replacement%20vector

Record number: 2, Textual support number: 1 CONT

Making more subtle mutations: the "knock in". Although it is often desirable to simply inactivate a gene to determine the importance of a null phenotype, in many cases it is more informative not to inactivate a gene, but rather to modify it so that its function is altered. Again, this is a task (which is the logical equivalent of site directed mutagenesis in a plasmid) that can be solved by homologous recombination. In this case a targeting vector is designed as a replacement vector so that additional genetic sequence are added into the genome. This technique is sometimes called a "knock-in".  2, record 2, English, - replacement%20vector

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 DEF

Véhicule de clonage comportant un ou plusieurs sites de coupure pour une enzyme de restriction donnée définissant ainsi un fragment d’ADN pouvant être éliminé et remplacé par de l'ADN étranger.  2, record 2, French, - vecteur%20de%20remplacement

Record number: 2, Textual support number: 1 CONT

Thomas et Capecchi ont au contraire muté le gène Hprt dans des cellules ES en utilisant deux types de construction déduits des mécanismes de recombinaison connus chez la levure : des vecteurs de remplacement, dont les séquences viennent remplacer les séquences homologues endogènes, et des vecteurs d’insertion, dont les séquences s’insèrent au milieu des séquences endogènes.  3, record 2, French, - vecteur%20de%20remplacement

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 DEF

A cloning vehicle which has a single cleavage site for one or more restriction endonucleases, thus allowing the insertion of a fragment of foreign DNA.  2, record 3, English, - insertion%20vector

Record number: 3, Textual support number: 1 CONT

Avipoxviruses, including Fowlpoxvirus (FPV) have been shown previously to infect mammalian cells but are unable to complete their replication cycle. Avipoxvirus based rabies and HIV vaccines have shown promise in preclinical studies. We have begun work to develop the FPV system for application to HPV CTL. We first constructed a new FPV insertion vector (Fig. 3) which facilitates production of recombinant viruses by co-insertion of the gpt marker gene under the control of a vaccinia virus 19K promoter. The gene of interest is inserted into a polylinker downstream of the vaccinia virus 7.5K promoter.  3, record 3, English, - insertion%20vector

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 DEF

Vecteur de clonage ne comportant qu'un seul site de coupure pour une enzyme de restriction donnée, permettant ainsi l'insertion d’un fragment d’ADN étranger.  2, record 3, French, - vecteur%20d%26rsquo%3Binsertion

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 OBS

Direction du service des laboratoires judiciaires, Gendarmerie royale du Canada.  2, record 4, French, - Forensic%20Analysis%20of%20Restriction%20Fragment%20Length%20Polymorphism%20%3A%20Theoretical%20and%20Potential%20Considerations%20for%20Design%20and%20Implementation

Record 4,  Textual support,  Spanish

Record 5,  Textual support,  French

Record 5,  Textual support,  Spanish