TERMIUM Plus®
The Government of Canada’s terminology and linguistic data bank.
SEQUENCE SITE RESTRICTION [3 records]
Record 1 - internal organization data 2012-08-31
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- linker
1, record 1, English, linker
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
A short, synthetic deoxyribonucleic acid (DNA) molecule that contains the recognition site for a specific restriction endonuclease. 2, record 1, English, - linker
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- lieur
1, record 1, French, lieur
correct, masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- adaptateur 2, record 1, French, adaptateur
correct, masculine noun
- séquence de liaison 1, record 1, French, s%C3%A9quence%20de%20liaison
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Oligonucléotide de synthèse de 4 ou 6 paires de désoxyribonucleotides dont la séquence correspond à un site reconnu par une endonucléase de restriction. 3, record 1, French, - lieur
Record number: 1, Textual support number: 1 OBS
Ces molécules peuvent être liées aux extrémités franches de n’importe quel segment d’ADN, ce qui facilitera son insertion dans un vecteur. 3, record 1, French, - lieur
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Genética
Record 1, Main entry term, Spanish
- ligador
1, record 1, Spanish, ligador
correct, masculine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 DEF
Oligonucleótido sintético de doble hebra, que contiene la secuencia de reconocimiento de una o más endonucleasas de restricción. 1, record 1, Spanish, - ligador
Record number: 1, Textual support number: 1 OBS
La unión de un ligador a cada extremo de un fragmento de ADN facilita la preparación del fragmento para su clonación en un vector. 1, record 1, Spanish, - ligador
Record 2 - internal organization data 2003-02-17
Record 2, English
Record 2, Subject field(s)
- Biological Sciences
Record 2, Main entry term, English
- site restriction sequence 1, record 2, English, site%20restriction%20sequence
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, French
Record 2, Domaine(s)
- Sciences biologiques
Record 2, Main entry term, French
- séquence de site de restriction
1, record 2, French, s%C3%A9quence%20de%20site%20de%20restriction
feminine noun
Record 2, Abbreviations, French
Record 2, Synonyms, French
Record 2, Textual support, French
Record 2, Spanish
Record 2, Campo(s) temático(s)
- Ciencias biológicas
Record 2, Main entry term, Spanish
- secuencia del sitio de restricción
1, record 2, Spanish, secuencia%20del%20sitio%20de%20restricci%C3%B3n
feminine noun
Record 2, Abbreviations, Spanish
Record 2, Synonyms, Spanish
Record 2, Textual support, Spanish
Record 3 - internal organization data 1996-07-12
Record 3, English
Record 3, Subject field(s)
- Genetics
Record 3, Main entry term, English
- mega-primer
1, record 3, English, mega%2Dprimer
correct
Record 3, Abbreviations, English
Record 3, Synonyms, English
Record 3, Textual support, English
Record 3, French
Record 3, Domaine(s)
- Génétique
Record 3, Main entry term, French
- méga-amorce
1, record 3, French, m%C3%A9ga%2Damorce
correct, feminine noun
Record 3, Abbreviations, French
Record 3, Synonyms, French
Record 3, Textual support, French
Record number: 3, Textual support number: 1 CONT
C'est pour répondre à ce problème que la technique dite de la méga-amorce a été mise au point. Dans un premier temps, la matrice d’ADN parental est amplifiée par une PCR utilisant comme amorces un oligonucléotide qui porte la mutation désirée, et un deuxième oligonucléotide situé à proximité d’un site de restriction X. Le produit de cette PCR est appelé la méga-amorce : il correspond à une partie de la séquence du gène, joignant la mutation et le site X. 1, record 3, French, - m%C3%A9ga%2Damorce
Record number: 3, Textual support number: 1 OBS
Technique utilisée en mutagénèse dirigée. 2, record 3, French, - m%C3%A9ga%2Damorce
Record 3, Spanish
Record 3, Textual support, Spanish
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