TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

a fluid obtained by breaking open cells contains most of the soluble molecules of a cell.  2, record 1, English, - cell%2Dfree%20extract

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 DEF

Extrait ne contenant pas de cellule.  2, record 1, French, - extrait%20acellulaire

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

Frozen cells (about 20 g wet weight) harvested from about 150 liter culture with sodium thiosulfate were suspended in 270 ml of 10 mM Tris-HCl buffer, pH 8.0, and then disrupted in a French pressure cell (1,100 kg/cm2). All subsequent steps were performed under aerobic conditions at room temperature. Unbroken cells were removed by centrifugation at 10,000 x g for 15 min. The resulting supernatant was used as a cell-free extract.  3, record 2, English, - cell%20free%20extract

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 DEF

Broyat cellulaire plus ou moins purifié capable d'assurer certaines fonctions enzymatiques du vivant in vitro.  2, record 2, French, - extrait%20acellulaire

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 DEF

Solution obtained by rupturing cells and removing all particulate matter.  2, record 3, English, - cell%2Dfree%20extract

Record number: 3, Textual support number: 1 CONT

Intracellular enzymes are usually released from inside the cell by mechanical or chemical disruption, or by permeabilization of the cell membrane so as to allow leakage. Common methods of cell disruption include high pressure homogenizers, ball mills, freeze-thawing, pH, temperature, or osmotic shocking, or enzyme digestion such as with lysozyme or the mixture of enzymes derived from Micrococcus lysodeikticus. The resulting crude enzymically active preparations are then clarified free of remaining cells and cell debris by filtration or centrifugation... The resulting cell-free extracts can then be purified by a consecutive series of fractionation and concentration steps. These include ion-exchange chromatography and membrane techniques, such as ultrafiltration and reverse osmosis.  3, record 3, English, - cell%2Dfree%20extract

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 DEF

Extrait dépourvu des structures de la cellule intacte. [...] A partir de telles préparations, il devient possible d'accumuler des intermédiaires métaboliques en utilisant des inhibiteurs enzymatiques spécifiques par inactivation d'enzymes spécifiques ou par élimination ou inactivation de coenzymes indispensables.  2, record 3, French, - extrait%20acellulaire

Record number: 3, Textual support number: 1 CONT

En 1897, la découverte par Buchner du fait que la cellule vivante per se n'est pas nécessaire à la fermentation fit faire un grand progrès à l'étude des étapes chimiques de ce processus. Il observa qu'un extrait acellulaire de levure était capable, par lui-même, de transformer le glucose en éthanol.  3, record 3, French, - extrait%20acellulaire

Record 3,  Textual support,  Spanish