TERMIUM Plus®

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GELOSE ADN [1 record]

Record 1 2023-05-16

English

Subject field(s)
  • Scientific Research Equipment
  • Medical and Surgical Equipment
  • Microbiology and Parasitology
CONT

DNase Agar. DM132 A medium for the presumptive identification of pathogenic staphylococci by demonstration of DNase production.

CONT

Deoxyribonuclease (DNAse) agar actually has DNA in it. Deoxyribonuclease is an exoenzyme excreted from the cell which will break the DNA down into smaller molecules. There are 2 kinds of DNAse agar that can be used. 1) The first medium has no indicator, but is does require HCl (hydrochloric acid) reagent to be added after growth has occurred on the plate. Without DNAse enzyme, the HCl precipitates out the undestroyed DNA. The rest of the agar with the remaining DNA, which binds to HCl will have turned cloudy. Around the growth area, if the bacterium has made DNAse and destroyed the DNA, there will be clear zones seen. 2) DNAse agar with the indicator methyl green can be used. In this medium, when the DNA in the medium is hydrolyzed by the bacterial DNAse enzyme, the pieces of cut DNA release from binding to methyl green, and you will see a yellowish/orange zone around the growth area. OBJECTIVE: To identify the presence of the DNAse enzyme.

PHR

DNase agar with (w/) toluidine blue, methyl green.

Key term(s)
  • desoxyribonucleic acid agar
  • desoxyribonuclease agar

French

Domaine(s)
  • Matériel et équipement (Recherche scientifique)
  • Équipement médico-chirurgical
  • Microbiologie et parasitologie
OBS

La gélose de test DNase est un milieu de culture pour détecter la production de désoxyribonucléase par des bactéries, en particulier les staphylocoques.

OBS

La désoxyribonucléase (ADNase [ou DNase]) est une enzyme qui catalyse les acides désoxyribonucléiques (ADN).

PHR

Gélose à ADN au bleu de toluidine, au vert de méthyle.

PHR

Gélose DNase au bleu de toluidine, au vert de méthyle.

Key term(s)
  • gélose ADNase
  • gélose désoxyribonucléique

Spanish

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