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TECHNIQUE PIEGEAGE GENES [1 record]

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Record 1 2017-01-10

English

Subject field(s)
  • Biotechnology
CONT

Gene trapping in murine embryonic stem cells is a proven method for the simultaneous identification and mutation of genes in the mouse. Gene trap vectors are designed to detect insertions within genes through the production of a fusion mRNA transcript, making the identification of the endogenous gene possible by 5' rapid amplification of cDNA ends (RACE).

French

Domaine(s)
  • Biotechnologie
CONT

La recherche de gènes essentiels au développement embryonnaire de la souris a été abordée par la mise en œuvre de stratégies variées. Comparée à toutes ces approches, la stratégie de piégeage de gènes présente un important avantage : elle associe l'isolement et la caractérisation moléculaire d'un gène à l'étude de son profil d'expression ainsi qu'à la détermination de sa fonction in vivo.

Spanish

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