TERMIUM Plus®

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CLONER [1 record]

Record 1 2009-06-19

English

Subject field(s)
  • Biochemistry
  • Genetics
DEF

To establish or produce ... a line of genetically identical molecules, cells or organisms asexually descended from a common ancestor.

CONT

To clone an animal, it is necessary to remove surgically, or totally inactivate with radiation, the nucleus in a fertilized egg, and replace it with a nucleus taken from another individual.

French

Domaine(s)
  • Biochimie
  • Génétique
DEF

Procéder au clonage de cellules.

CONT

Pour cloner dans une bactérie le DNA codant pour une protéine de mammifère, il convient d'abord d'identifier et de purifier le mieux possible le mRNA correspondant [...]; de synthétiser ensuite le cDNA [complémentaire ADN] complémentaire à ce mRNA [messager RNA]; d'insérer ce cDNA dans un plasmide [...] qui est ensuite introduit dans des bactéries; puis de sélectionner soigneusement une ou plusieurs souches bactériennes contenant le plasmide avec le bon DNA à cloner; enfin d'amplifier ce dernier plasmide et de le purifier.

Spanish

Campo(s) temático(s)
  • Bioquímica
  • Genética
DEF

Derivar una población celular a partir de una sola célula.

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