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HYBRIDATION SOUSTRACTIVE [1 record]
Record 1 - internal organization data 2011-11-02
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- substractive hybridization
1, record 1, English, substractive%20hybridization
correct
Record 1, Abbreviations, English
- SH 2, record 1, English, SH
correct
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Animal and human (normal and osteoarthritic) chondrocytes can now be cultivated under various physical and chemical conditions in the presence of active exogenous factors, such as growth factors, vitamins, and anti-osteoarthritis preparations, owing to the development of culture models including organotypic, high density and three-dimensional models. The molecular biology approach to this research ... has resulted in stable lines of chondrocytes (e.g. MC 615 from mice) and a comparison of the genetic expression of these cells at different stages of differentiation by substractive hybridization or differential PCR screening. 3, record 1, English, - substractive%20hybridization
Record number: 1, Textual support number: 2 CONT
Differential gene expression occurs in the process of development, maintenance, injury, and death of unicellular as well as complex organisms. Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Electronic substraction (ES), substractive hybridization (SH), and differential display (DD) are methods commonly used for this purpose. 4, record 1, English, - substractive%20hybridization
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- hybridation soustractive
1, record 1, French, hybridation%20soustractive
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- technique des banques soustractives 1, record 1, French, technique%20des%20banques%20soustractives
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
À ce jour, les études comparatives de l'expression génique reposent essentiellement sur l'hybridation soustractive (ou technique de banques soustractives). Cette technique permet de détecter les espèces d'ARNm présentes dans une cellule particulière par différence avec les ARNm d'une cellule prise comme référence. Le séquençage des ADNc complémentaires obtenus, puis leur comparaison avec les banques de gènes permet l'identification des ARNm surexprimés dans la cellule étudiée. 1, record 1, French, - hybridation%20soustractive
Record number: 1, Textual support number: 1 OBS
Outil d'expression génique. 2, record 1, French, - hybridation%20soustractive
Record 1, Spanish
Record 1, Textual support, Spanish
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