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LYMPHOCYTOTOXICITE [4 records]

Record 1 2002-07-30

English

Subject field(s)
  • Immunology
CONT

[In] the crossmatch assay ... patient sera are tested for their reactivity with donor lymphocytes. This is usually done by lymphocytotoxicity testing whereby donor lymphocytes are first incubated with patient serum, then with rabbit complement and lysis of lymphocytes is assessed by the uptake of an extravital dye like trypan blue or eosin red.

French

Domaine(s)
  • Immunologie
CONT

Les Ac anti-HLA [anticorps anti-Human Leukocyte Antigen] s'acquièrent lors de transfusions, de grossesses ou de transplantations, on les recherche par une réaction de lyse cellulaire (lymphocytotoxicité) ou par immunofluorescence.

Spanish

Save record 1

Record 2 1993-03-25

English

Subject field(s)
  • Immunology

French

Domaine(s)
  • Immunologie
DEF

Le test de lymphocytotoxicité consiste à incuber, dans un premier temps, les lymphocytes avec le sérum du malade inactivé, puis à ajouter du complément. La mort cellulaire est évaluée, en général, par la prise de bleu tryptan, colorant qui ne pénètre que dans les cellules mortes. Une autre technique consiste à mesurer la libération de chrome 51, préalablement fixé sur les lymphocytes.

Spanish

Save record 2

Record 3 1993-03-17

English

Subject field(s)
  • Lymphatic System
DEF

The quality or capability of lyzing lymphocytes, as in procedures in which lymphocytes having a specific cell surface antigen are lyzed when incubated with antiserum and complement.

French

Domaine(s)
  • Système lymphatique
DEF

Effet de cytotoxicité exercé directement par des lymphocytes sur une cellule cible, en l'absence de complément.

CONT

(Méthode de) séparation des sous-populations de cellules T utilisant les anticorps monoclonaux. (...) La lymphocytotoxicité consiste à incuber la suspension cellulaire à éliminer avec l'anticorps monoclonal en présence de complément. Les cellules non reconnues par l'anticorps sont sélectionnées négativement. (J.-F. Bach, P. Lesavre, Op. cit., p. 24).

Spanish

Save record 3

Record 4 1993-03-05

English

Subject field(s)
  • Immunology
DEF

A variation of the mixed lymphocyte culture (MLC) techniques that is a functional test of the ability of cytotoxic lymphocytes (CTL) to kill target cells. Lymphocytes from two individuals are cultured together for several days, one population having been prevented from proliferating by treatment with radiation or mitomycin (a "one-way" MLC); they are then cultured for several hours with 51CR-labeled target cells that are HLA-identical to the stimulator cells. Cytotoxicity is measured as percentage of 51CR released from specific target cells compared to percentage of 51CR released from control (nonspecific target) cells.

French

Domaine(s)
  • Immunologie
CONT

Test de cytotoxicité à médiation lymphocytaire. Lorsque des lymphocytes provenant de deux populations allogéniques différentes sont mis en présence dans un tube, on assiste à l'apparition de lymphoblastes (ce qui définit la réaction lymphocytaire mixte), mais aussi à celle d'une génération de cellules T cytotoxiques spécifiques. C'est la réaction de lymphocytotoxicité à médiation cellulaire appelée en anglais "cell mediated lympholysis".

Spanish

Save record 4

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