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MICRODISSECTION REGION CHROMOSOMIQUE [1 record]
Record 1 - internal organization data 1996-09-30
Record 1, English
Record 1, Subject field(s)
- Biotechnology
- Genetics
Record 1, Main entry term, English
- single-chromosome microdissection
1, record 1, English, single%2Dchromosome%20microdissection
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
One approach is single-chromosome microdissection, in which a piece is physically cut from a chromosomal region of particular interest, broken up into smaller pieces, and amplified by PCR or cloning. These fragments can then be mapped and sequenced by the methods previously described. 1, record 1, English, - single%2Dchromosome%20microdissection
Record 1, French
Record 1, Domaine(s)
- Biotechnologie
- Génétique
Record 1, Main entry term, French
- microdissection d’une région chromosomique
1, record 1, French, microdissection%20d%26rsquo%3Bune%20r%C3%A9gion%20chromosomique
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- micro-dissection d’une région chromosomique 2, record 1, French, micro%2Ddissection%20d%26rsquo%3Bune%20r%C3%A9gion%20chromosomique
correct, feminine noun, obsolete
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La micro-dissection d'une région chromosomique [...] a été obtenue pour la première fois par Lüdecke et al (1989) [...] Le produit de la dissection de 20 à 40 chromosomes a été digéré par une enzyme de restriction coupant fréquemment, et les fragments obtenus ont été liés à des amorces universelles de clonage, permettant d'effectuer ensuite une micro-amplification par PCR en utilisant ces mêmes amorces. Après clonage du produit d'amplification dans un plasmide, 20 000 clones ont été obtenus. 2, record 1, French, - microdissection%20d%26rsquo%3Bune%20r%C3%A9gion%20chromosomique
Record 1, Spanish
Record 1, Textual support, Spanish
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