TERMIUM Plus®

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MICROSCOPE STED [1 record]

Record 1 2015-09-09

English

Subject field(s)
  • Scientific Instruments
  • Optics
  • Biophysics
  • Molecular Biology
DEF

A scanning laser microscope that uses a short excitation pulse followed by a depletion pulse tuned to an emission line of a fluorescent dye.

CONT

Using custom-made STED microscopes with a lateral resolution of fewer than 50 nm [nanometers], mitochondrial proteins Tom20, VDAC1, and COX2 were found to distribute as nanoscale clusters.

French

Domaine(s)
  • Instruments scientifiques
  • Optique
  • Biophysique
  • Biologie moléculaire
CONT

Le microscope STED est basé sur un microscope à fluorescence confocal à balayage. Pour mieux comprendre le principe du STED, il est utile de réviser les principes de fluorescence, émission stimulée et microscopie confocale.

Spanish

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