TERMIUM Plus®

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PRECIPITATION SELS [2 records]

Filter results by subject field Alphabetical list of terms

Filter results by subject field

Alphabetical list of terms

Record 1 2001-12-04

English

Subject field(s)
  • Biochemistry
DEF

Procedure in which salts are removed from a mixed solution of high molecular weight biological molecules.

French

Domaine(s)
  • Biochimie
DEF

Opération qui permet l'élimination des sels des solutions protéiques.

Spanish

Campo(s) temático(s)
  • Bioquímica
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Record 2 1989-12-19

English

Subject field(s)
  • Biological Sciences
DEF

The decrease in the solubility of a protein that is produced in solutions of high ionic strength by an increase of the concentrations of neutral salts.

CONT

The physicochemical basis of salting-out is rather complex; one factor is that the high concentration of salt may remove water of hydration from the protein molecules, thus reducing their solubility, but other factors are also involved. Whatever their physical basis, salting-in and salting-out are important procedures in the separation of protein mixtures, since different proteins vary in their response to the concentration of neutral salts. Proteins precipitated by salting-out retain their native conformation and can be dissolved again, usually without denaturation. Ammonium sulfate is preferred for salting out proteins because it is so soluble in water that very high ionic strengths can be attained.

French

Domaine(s)
  • Sciences biologiques
DEF

Précipitation réversible des protéines par de fortes concentrations de sels minéraux.

CONT

Le relarguage (...) des protéines à force ionique élevée varie suivant les protéines. Il est donc possible, par un accroissement progressif de la concentration saline (sulfate d'ammonium ou de sodium), d'obtenir une précipitation fractionnée des diverses protéines. En fait, par ce procédé, on obtient presque toujours des mélanges de protéines.

OBS

Certains ouvrages français utilisent le terme "salting-out".

Spanish

Save record 2

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