TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

A replicon used for the transformation of cells in gene manipulation.  4, record 1, English, - cloning%20vehicle

Record number: 1, Textual support number: 1 OBS

Small plasmids, viruses and bacteriophages are suitable, since they are replicons in their own right. They can be artificially constructed by cutting and joining DNA molecules from different sources using restriction enzymes and ligases.  4, record 1, English, - cloning%20vehicle

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 DEF

Molécule d'ADN capable d'autoréplication et sur laquelle il est possible de cloner des fragments d'ADN qui peuvent ensuite être propagés dans une cellule hôte.  4, record 1, French, - v%C3%A9hicule%20de%20clonage

Record number: 1, Textual support number: 1 OBS

Le bactériophage lambda et les plasmides sont des vecteurs utilisés pour cloner des fragments d'ADN dans Escherichia coli.  4, record 1, French, - v%C3%A9hicule%20de%20clonage

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 DEF

Molécula pequeña de ADN autorreplicativa, normalmente un plásmido o un fragmento de ADN vírico, en la que se inserta ADN exógeno en el proceso de clonar genes u otras secuencias de ADN de interés.  2, record 1, Spanish, - vector%20de%20clonaci%C3%B3n

Record number: 1, Textual support number: 1 OBS

Transporta el ADN insertado y puede perpetuarse en una célula hospedadora.  2, record 1, Spanish, - vector%20de%20clonaci%C3%B3n

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

The purification and isolation of individual fragments of DNA or a mixture of DNA fragments by their incorporation into a carrier DNA (vector) to form a recombinant DNA that can be multiplied up into a bacterial host.  1, record 2, English, - DNA%20cloning

Record number: 2, Textual support number: 1 OBS

Not to be confused with "gene cloning" which is defined as the isolation of DNA into a particular gene.  2, record 2, English, - DNA%20cloning

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 DEF

Technique qui permet d'obtenir de nombreuses copies d'un ADN donné.  2, record 2, French, - clonage%20de%20l%27ADN

Record 2,  Textual support,  Spanish

Record number: 2, Textual support number: 1 DEF

Síntesis de múltiples copias de una secuencia seleccionada de ADN utilizando una célula bacteriana u otro organismo como hospedador; el gen se inserta en un vector y la molécula resultante de ADN recombinante se amplifica en una célula hospedadora apropiada.  1, record 2, Spanish, - clonaci%C3%B3n%20de%20ADN

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 DEF

The addition of complementary bases to the ends of blunt-ended foreign and vector DNA sequences so that they may base-pair to form a recombinant DNA molecule.  1, record 3, English, - homopolymer%20tailing

Record number: 3, Textual support number: 1 OBS

For example, poly(dG) [polydeoxyguanylic acid] may be added to the ends of the foreign DNA while poly(dC) [polydeoxycytidylic acid] is added to ends of the vector DNA. The enzyme catalysing these transfer reactions is called terminal transferase.  1, record 3, English, - homopolymer%20tailing

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 CONT

L'ADNc double brin [...] est inséré dans un vecteur, la plupart du temps pBR322 [plasmide de Bolivar et Rodriguez]. Deux types de méthodes sont utilisées pour ce faire : celle des adaptateurs ajoutés à chacune des extrémités des fragments, ou celle des séquences homopolymériques complémentaires.  3, record 3, French, - extension%20homopolym%C3%A9rique

Record 3,  Textual support,  Spanish

Record number: 3, Textual support number: 1 DEF

Adición in vitro de múltiples copias de un único nucleótido a los extremos 3'-hidroxilo de una molécula de ADN bicatenario por acción de la enzima transferasa terminal.  1, record 3, Spanish, - prolongaci%C3%B3n%20con%20homopol%C3%ADmeros

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 CONT

The Bartels PRIMA SystemTM Cytomegalovirus IgM Enzyme Immunoassay is a rapid, qualitative method for the detection of IgM Antibody to cytomegalovirus in human serum. Patient serum is diluted with specimen diluent that contains an absorbent to remove IgG. The absorbent prevents IgG/rheumatoid factor complexes from forming and prevents interfering competition from IgG during the test procedure. The treated serum is incubated with purified CMV antigens bound to EIA plate wells. If antibodies to CMV are present, they bind to the antigen and do not rinse off. Subsequently, when enzyme labeled anti-human IgM is added to the reaction site, it binds to the immobilized antibodies. After washing and the addition of a chromogenic substrate and stop solution, specimens containing antibodies to CMV produce a color endpoint reaction which is measured and compared to negative and positive controls, and may be measured spectrophotometrically.  1, record 4, English, - chromogenic%20substrate

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Dans ce cas, les dNTP (2-désoxyribonucléotides triphosphates peuvent être marqués par la biotine [...] Les produits d'amplification sont ensuite détectés directement par l'avidine ou la streptavidine couplée à la phosphatase alcaline ou à la peroxydase et le substrat chromogène correspondant.  1, record 4, French, - substrat%20chromog%C3%A8ne

Record 4,  Textual support,  Spanish

Record number: 4, Textual support number: 1 DEF

Compuesto o sustancia que contiene un grupo que produce color.  1, record 4, Spanish, - sustrato%20cromog%C3%A9nico

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 DEF

A piece of DNA made to contain many restriction enzyme sites, so that the vector can be cut at that one place for splicing into other genes.  2, record 5, English, - polylinker

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 DEF

Lieur possédant plusieurs sites de restriction.  1, record 5, French, - lieur%20multisite

Record 5,  Textual support,  Spanish

Record number: 5, Textual support number: 1 DEF

Segmento sintético de ADN diseñado para incluir varios sitios de restricción diferentes.  1, record 5, Spanish, - poliligador

Record number: 5, Textual support number: 1 OBS

En los procesos de clonación, su unión con el fragmento de ADN que se desea clonar, permite elegir entre muchas endonucleasas de restricción.  1, record 5, Spanish, - poliligador