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R BAND [1 record]

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Record 1 2010-08-11

English

Subject field(s)
  • Biotechnology
CONT

The molar fractions of guanine plus cytosine (GC) in DNA were determined for 36 yeast artificial chromosomes (YACs) which almost completely cover human chromosome band Xq28, a terminal reverse band, corresponding to about 8 Mb of DNA. This allowed the construction of the most complete compositional map to date of a chromosomal band...

OBS

Proceedings of the National Academy of Sciences, Volume 93, Number 03; Pages: 1298-1302

French

Domaine(s)
  • Biotechnologie
CONT

Les chromosomes métaphasiques humains présentent divers types de bandes selon le traitement auquel ils sont soumis (colorants, température, enzymes protéolytiques ou DNases). Les bandes G(iemsa) positives représentent environ 50 % des bandes chromosomiques, l'autre moitié étant constituée de bandes G(iemsa) négatives. Ces dernières ne sont que les bandes R(everse) obtenues par dénaturation thermique.

OBS

Elles sont obtenues par dénaturation thermique en solution saline, spécifiques des régions riches en guanine et en cytosine ainsi qu'en gènes de synthèse précoces.

Spanish

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