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COMPLEMENTATION CELL [1 record]
Record 1 - internal organization data 2004-06-30
Record 1, English
Record 1, Subject field(s)
- Genetics
- Biotechnology
Record 1, Main entry term, English
- packaging cell
1, record 1, English, packaging%20cell
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- complementation cell 2, record 1, English, complementation%20cell
correct
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Treated with these two genomes, the packaging cell produces a crop of retroviruses with: the envelope protein needed to infect the human target cells; an RNA copy of the human ADA [adenosine deaminase] gene, complete with R sequences at each end; reverse transcriptase, needed to make a DNA copy of the ADA gene that can be inserted into the DNA of the target cell and none of the genes (gag, pol, env) that would enable the virus to replicate in its new host. 3, record 1, English, - packaging%20cell
Record number: 1, Textual support number: 2 CONT
To create the retroviral vectors that are used in gene therapy, the life-cycles of their naturally occurring counterparts are exploited. The transgene (in this case, the gene for factor IX) in a vector backbone is put into a packaging cell, which expresses the genes that are required for viral integration. 4, record 1, English, - packaging%20cell
Record 1, French
Record 1, Domaine(s)
- Génétique
- Biotechnologie
Record 1, Main entry term, French
- cellule d'encapsidation
1, record 1, French, cellule%20d%27encapsidation
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- cellule transcomplémentante 2, record 1, French, cellule%20transcompl%C3%A9mentante
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Les différents modes de transfert sont : [...] Injection de cellules produisant le vecteur viral pour éviter son inactivation trop rapide par le complément. Ces cellules sont appelés cellules transcomplémentantes (ou cellules d'encapsidation). 3, record 1, French, - cellule%20d%27encapsidation
Record number: 1, Textual support number: 1 OBS
Il s'agit d'un type de cellules utilisées pour produire des virus non pathogènes en supprimant les gènes nécessaires à leur réplication et en les remplaçant par une séquence choisie et des éléments régulateurs. 4, record 1, French, - cellule%20d%27encapsidation
Record 1, Spanish
Record 1, Textual support, Spanish
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