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NUCLEIC ACID SEQUENCE BASED AMPLIFICATION [1 record]

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Record 1 2011-10-28

English

Subject field(s)
  • Biotechnology
  • Molecular Biology
Key term(s)
  • nucleic acid sequence-based amplification

French

Domaine(s)
  • Biotechnologie
  • Biologie moléculaire
CONT

La PCR [réaction en chaîne de la polymérase], qui permet l'amplification d'une séquence d'ADN en utilisant une polymérase d'ADN résistante à haute température, a révolutionné la biologie moléculaire. Sa spécificité dépend d'oligonucléotides se liant à l'ADN et délimitant la région qui va être amplifiée. Elle apporte un énorme gain de sensibilité par rapport aux méthodes précédentes et permet d'analyser le contenu en ADN à partir de quelques cellules seulement.

Spanish

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